View our list of available Coronavirus (COVID-19, SARS-CoV-2, 2019-nCOV) antibodies, recombinant proteins, ELISA Kits... Options Full Datasheet Printer Friendly Datasheet Prepare for Printing Full Datasheet Printer Friendly Datasheet Prepare for Printing Have Questions? Ask Us! Request A Quote Today! Add To Compare List PRL elisa kit :: Bovine Prolactin ELISA Kit Catalog # MBS721395 Unit / Price 48-Strip-Wells / $495 +1 FREE 8GB USB 96-Strip-Wells / $700 +1 FREE 8GB USB 5x96-Strip-Wells / $3,105 +3 FREE 8GB USB 10x96-Strip-Wells / $5,865 +7 FREE 8GB USB Product Name Product Info Accession #s Product Desc Diseases/Tissues/Pathways Applications References Product Name Prolactin (PRL), ELISA Kit Popular Item Full Product Name Bovine Prolactin ELISA Kit Product Gene Name PRL elisa kit[Similar Products] Research Use Only For Research Use Only. Not for use in diagnostic procedures. TOP Sample Manual Insert Download Sample PDF Manual View Sample PDF Manual OMIM 176760 Species Reactivity Bovine Specificity This assay has high sensitivity and excellent specificity for detection of PRL. No significant cross-reactivity or interference between PRL and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between PRL and all the analogues, therefore, cross reaction may still exist in some cases. Samples Serum, plasma, cell culture supernatants, body fluid and tissue homogenate Assay Type Quantitative Competitive Sensitivity 1.0 ng/mL Preparation and Storage Store all reagents at 2-8 degree C. Sample Preparation We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team ISO Certification Manufactured in an ISO 13485:2016 Certified Laboratory. Product Note Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details. Other Notes Small volumes of PRL elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply. Searchable Terms for PRL purchase MBS721395 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Prolactin (PRL) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing PRL. The ELISA analytical biochemical technique of the MBS721395 kit is based on PRL antibody-PRL antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect PRL antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, PRL. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%). TOP Related Product Information for PRL elisa kit Intended Uses: This PRL ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Bovine PRL. This ELISA kit for research use only, not for therapeutic or test applications!Principle of the Assay: PRL ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-PRL antibody and an PRL-HRP conjugate. The assay sample and buffer are incubated together with PRL-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the PRL concentration since PRL from samples and PRL-HRP conjugate compete for the anti-PRL antibody binding site. Since the number of sites is limited, as more sites are occupied by PRL from the sample, fewer sites are left to bind PRL-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PRL concentration in each sample is interpolated from this standard curve. Typical Testing Data/Standard Curve (for reference only) of PRL elisa kit TOP Sample Manual Insert of MBS721395. NCBI/Uniprot data below describe general gene information for PRL. It may not necessarily be applicable to this product. NCBI GI # 130930 NCBI GeneID 5617 NCBI Accession # P01236.1 [Other Products] UniProt Secondary Accession # Q15199; Q92996[Other Products] UniProt Related Accession # P01236[Other Products] Molecular Weight 25,876 Da TOP NCBI Official Full Name Prolactin NCBI Official Synonym Full Names prolactin NCBI Official Symbol PRL [Similar Products] NCBI Protein Information prolactin; decidual prolactin UniProt Protein Name Prolactin Protein Family Prolactin UniProt Gene Name PRL [Similar Products] UniProt Synonym Gene Names PRL [Similar Products] UniProt Entry Name PRL_HUMAN TOP NCBI Summary for PRL This gene encodes the anterior pituitary hormone prolactin. This secreted hormone is a growth regulator for many tissues, including cells of the immune system. It may also play a role in cell survival by suppressing apoptosis, and it is essential for lactation. Alternative splicing results in multiple transcript variants that encode the same protein. [provided by RefSeq, Aug 2011] TOP UniProt Comments for PRL prolactin: Prolactin acts primarily on the mammary gland by promoting lactation. Belongs to the somatotropin/prolactin family.Protein type: Motility/polarity/chemotaxis; Secreted; Secreted, signal peptide; CytokineChromosomal Location of Human Ortholog: 6p22.3Cellular Component: extracellular regionMolecular Function: protein binding; prolactin receptor binding; hormone activityBiological Process: lactation; cell proliferation; cell surface receptor linked signal transduction; regulation of multicellular organism growth; positive regulation of JAK-STAT cascade; female pregnancy Product References and Citations for PRL elisa kit Age-related and seasonal variations in plasma uncarboxylated osteocalcin in male Murrah buffaloes. B. S. Bharath Kumar, Vyankat Gangadhar Jadhav & Sujata Pandita (2017) Research Articles on PRL 1. Prolactin-Stat5 signaling in breast cancer is potently disrupted by acidosis within the tumor microenvironment. TOP Precautions All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines. Disclaimer While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. 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TOP Products associated with PRL elisa kit Reference Product PubMed Publications FSH elisa kit >2321 publications with PRL and FSH PRLR elisa kit >339 publications with PRL and PRLR JAK2 elisa kit >183 publications with PRL and JAK2 POU1F1 elisa kit >139 publications with PRL and POU1F1 STAT3 elisa kit >60 publications with PRL and STAT3 GHR elisa kit >52 publications with PRL and GHR ESR1 elisa kit >11 publications with PRL and ESR1 PTPN11 elisa kit >6 publications with PRL and PTPN11 Pathways associated with PRL elisa kit Products by Pathway Pathway Diagram Amyloids Pathway antibodies Amyloids Pathway Diagram Cytokine Signaling In Immune System Pathway antibodies Cytokine Signaling In Immune System Pathway Diagram Cytokine-cytokine Receptor Interaction Pathway antibodies Cytokine-cytokine Receptor Interaction Pathway Diagram Cytokine-cytokine Receptor Interaction Pathway antibodies Cytokine-cytokine Receptor Interaction Pathway Diagram Disease Pathway antibodies Disease Pathway Diagram ErbB4 Signaling Events Pathway antibodies ErbB4 Signaling Events Pathway Diagram Glucocorticoid Receptor Regulatory Network Pathway antibodies Glucocorticoid Receptor Regulatory Network Pathway Diagram Growth Hormone Receptor Signaling Pathway antibodies Growth Hormone Receptor Signaling Pathway Diagram Immune System Pathway antibodies Immune System Pathway Diagram Jak-STAT Signaling Pathway antibodies Jak-STAT Signaling Pathway Diagram Diseases associated with PRL elisa kit Disease Name Pubmed Publications Pituitary Neoplasms Antibodies >2009 publications with PRL and Pituitary Neoplasms Hyperprolactinemia Antibodies >1506 publications with PRL and Hyperprolactinemia Adenoma Antibodies >1473 publications with PRL and Adenoma Prolactinoma Antibodies >667 publications with PRL and Prolactinoma Mental Disorders Antibodies >599 publications with PRL and Mental Disorders Amenorrhea Antibodies >427 publications with PRL and Amenorrhea Galactorrhea Antibodies >316 publications with PRL and Galactorrhea Growth Hormone-Secreting Pituitary Adenoma Antibodies >260 publications with PRL and Growth Hormone-Secreting Pituitary Adenoma Infertility, Female Antibodies >232 publications with PRL and Infertility, Female Hypogonadism Antibodies >204 publications with PRL and Hypogonadism Organs/Tissues associated with PRL elisa kit Organ/Tissue Name Pubmed Publications Brain Antibodies >5093 publications with PRL and Brain Pituitary Gland Antibodies >3970 publications with PRL and Pituitary Gland Uterus Antibodies >515 publications with PRL and Uterus Testis Antibodies >491 publications with PRL and Testis Placenta Antibodies >387 publications with PRL and Placenta Heart Antibodies >206 publications with PRL and Heart Muscle Antibodies >185 publications with PRL and Muscle Eye Antibodies >185 publications with PRL and Eye Connective Tissue Antibodies >118 publications with PRL and Connective Tissue Uncharacterized Tissue Antibodies >1 publications with PRL and Uncharacterized Tissue TOP © 2006-24 MyBioSource.com. 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