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SPB elisa kit :: Bovine Pulmonary Surfactant Associated Protein B ELISA Kit

Scan QR to view Datasheet Catalog #    MBS735223
Typical Testing Data/Standard Curve
Unit / Price
48-Strip-Wells  /  $440 +1 FREE 8GB USB
96-Strip-Wells  /  $640 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,895 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,415 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Pulmonary Surfactant Associated Protein B (SPB), ELISA Kit

★Popular Item★
 Also Known As   

Bovine Pulmonary Surfactant Associated Protein B ELISA Kit

 Product Gene Name   

SPB elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 Species Reactivity    Bovine
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 Specificity    This assay has high sensitivity and excellent specificity for detection of SPB. No significant cross-reactivity or interference between SPB and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between SPB and all the analogues, therefore, cross reaction may still exist in some cases.
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 Samples    Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
 Assay Type    Competitive
 Detection Range    2.5-50ng/mL
 Sensitivity    1.0ng/mL
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 Preparation and Storage    Store all reagents at 2-8 degree C.
 Sample Preparation    We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of SPB elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for SPB purchase    MBS735223 is a ready-to-use microwell, strip plate Competitive ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Pulmonary Surfactant Associated Protein B (SPB) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 2.5-50ng/mL in biological research samples containing SPB, with an estimated sensitivity of 1.0ng/mL. The ELISA analytical biochemical technique of the MBS735223 kit is based on SPB antibody-SPB antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect SPB antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, SPB. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for SPB elisa kit

   Intended Uses: This SPB ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Bovine SPB. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Principle of the Assay||SPB ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for SPB. Standards or samples are then added to the microtiter plate wells and SPB if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of SPB present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for SPB are added to each well to "sandwich" the SPB immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain SPB and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The SPB concentration in each sample is interpolated from this standard curve.
 Product Categories/Family for SPB elisa kit    Immunology
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 Typical Testing Data/Standard Curve of SPB elisa kit    SPB elisa kit Typical Testing Data/Standard Curve image
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Sample Manual Insert of MBS735223. Click to request current manual
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NCBI/Uniprot data below describe general gene information for SPB. It may not necessarily be applicable to this product.
 NCBI GI #    126723505
 NCBI GeneID    100009194
 NCBI Accession #    NP_001075812.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001082343.1 [Other Products]
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 UniProt Secondary Accession #    P79333 [Other Products]
 UniProt Related Accession #    P15285 [Other Products]
 Molecular Weight    40,610 Da
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 NCBI Official Full Name    pulmonary surfactant-associated protein B
 NCBI Official Symbol    SFTPB [Similar Products]
 NCBI Official Synonym Symbols   
SP-B; SFTP3
[Similar Products]
 NCBI Protein Information    pulmonary surfactant-associated protein B; 6 kDa protein; pulmonary surfactant-associated proteolipid SPL(Phe)
 UniProt Protein Name    Pulmonary surfactant-associated protein B
 UniProt Synonym Protein Names   
6 kDa protein; Pulmonary surfactant-associated proteolipid SPL(Phe)
 UniProt Gene Name    SFTPB [Similar Products]
 UniProt Synonym Gene Names    SFTP3; SP-B [Similar Products]
 UniProt Entry Name    PSPB_RABIT
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 UniProt Comments for SPB    Pulmonary surfactant-associated proteins promote alveolar stability by lowering the surface tension at the air-liquid interface in the peripheral air spaces. SP-B increases the collapse pressure of palmitic acid to nearly 70 millinewtons per meter.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with SPB elisa kitDiseases associated with SPB elisa kit
 Reference Product  PubMed Publications
 SFTPC elisa kit  >29 publications with SPB and SFTPC
 SFTPA1 elisa kit  >7 publications with SPB and SFTPA1
 SFTPD elisa kit  >7 publications with SPB and SFTPD
 CLDN18 elisa kit  >1 publications with SPB and CLDN18
 SCGB1A1 elisa kit  >1 publications with SPB and SCGB1A1
 SLC34A2 elisa kit  >1 publications with SPB and SLC34A2
 Disease Name  Pubmed Publications
 Lung Diseases Antibodies  >49 publications with SPB and Lung Diseases
 Lung Neoplasms Antibodies  >9 publications with SPB and Lung Neoplasms
 Inflammation Antibodies  >7 publications with SPB and Inflammation
 Lung Injury Antibodies  >6 publications with SPB and Lung Injury
 Necrosis Antibodies  >3 publications with SPB and Necrosis
 Pneumonia Antibodies  >3 publications with SPB and Pneumonia
 Fibrosis Antibodies  >2 publications with SPB and Fibrosis
 Hypertension Antibodies  >2 publications with SPB and Hypertension
 Pulmonary Fibrosis Antibodies  >2 publications with SPB and Pulmonary Fibrosis
 Brain Diseases Antibodies  >1 publications with SPB and Brain Diseases
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