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REG3G elisa kit :: Bovine Regenerating islet-derived protein 3-gamma ELISA Kit

Scan QR to view Datasheet Catalog #    MBS755934
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $440 +1 FREE 8GB USB
96-Strip-Wells  /  $640 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,895 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,415 +6 FREE 8GB USB
 
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 Product Name   

Regenerating islet-derived protein 3-gamma (REG3G), ELISA Kit

★Popular Item★
 Also Known As   

Bovine Regenerating islet-derived protein 3-gamma ELISA Kit

 Product Gene Name   

REG3G elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    609933
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 Species Reactivity    Bovine
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 Specificity    This assay has high sensitivity and excellent specificity for detection of REG-3?. No significant cross-reactivity or interference between REG-3? and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between REG-3? and all the analogues, therefore, cross reaction may still exist in some cases.
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 Samples    Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
 Assay Type    Competitive
 Detection Range    1.0-25ng/mL
 Sensitivity    0.1ng/mL
 Intended Uses    This REG-3? ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Bovine REG-3?. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
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 Preparation and Storage    Store all reagents at 2-8 degree C.
 Sample Preparation    We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of REG3G elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for REG3G purchase    MBS755934 is a ready-to-use microwell, strip plate Competitive ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Regenerating islet-derived protein 3-gamma (REG3G) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 1.0-25ng/mL in biological research samples containing REG3G, with an estimated sensitivity of 0.1ng/mL. The ELISA analytical biochemical technique of the MBS755934 kit is based on REG3G antibody-REG3G antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect REG3G antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, REG3G. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for REG3G elisa kit

   Principle of the assay: REG-3? ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-REG-3? antibody and an REG-3?-HRP conjugate. The assay sample and buffer are incubated together with REG-3?-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the REG-3? concentration since REG-3? from samples and REG-3?-HRP conjugate compete for the anti-REG-3? antibody binding site. Since the number of sites is limited, as more sites are occupied by REG-3? from the sample, fewer sites are left to bind REG-3?-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The REG-3? concentration in each sample is interpolated from this standard curve.
 Product Categories/Family for REG3G elisa kit    Cell Biology
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 Typical Testing Data/Standard Curve (for reference only) of REG3G elisa kit    REG3G elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS755934. Click to request current manual
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NCBI/Uniprot data below describe general gene information for REG3G. It may not necessarily be applicable to this product.
 NCBI GI #    56699413
 NCBI GeneID    130120
 NCBI Accession #    NP_001008388.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001008387.2 [Other Products]
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 UniProt Secondary Accession #    Q3SYE4; Q3SYE6; Q6FH18; A8K980; D6W5J6 [Other Products]
 UniProt Related Accession #    Q6UW15 [Other Products]
 Molecular Weight    14,409 Da
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 NCBI Official Full Name    regenerating islet-derived protein 3-gamma isoform 1
 NCBI Official Synonym Full Names    regenerating islet-derived 3 gamma
 NCBI Official Symbol    REG3G [Similar Products]
 NCBI Official Synonym Symbols   
PAP1B; PAPIB; PAP IB; PAP-1B; UNQ429; LPPM429; REG III; REG-III
[Similar Products]
 NCBI Protein Information    regenerating islet-derived protein 3-gamma; REG-3-gamma; reg III-gamma; regenerating gene III; pancreatitis-associated protein 1B; pancreatitis-associated protein IB; regenerating islet-derived protein III-gamma
 UniProt Protein Name    Regenerating islet-derived protein 3-gamma
 UniProt Synonym Protein Names   
Pancreatitis-associated protein 1B; PAP-1B; Pancreatitis-associated protein IB; PAP IB; Regenerating islet-derived protein III-gamma; REG III; Reg III-gamma
 Protein Family    Regenerating islet-derived protein
 UniProt Gene Name    REG3G [Similar Products]
 UniProt Synonym Gene Names    PAP1B; UNQ429/PRO162; REG-3-gamma; PAP-1B; PAP IB; REG III; Reg III-gamma [Similar Products]
 UniProt Entry Name    REG3G_HUMAN
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 NCBI Summary for REG3G    This gene encodes a member of the regenerating islet-derived genes (REG)3 protein family. These proteins are secreted, C-type lectins with a carbohydrate recognition domain and N-terminal signal peptide. The protein encoded by this gene is an antibacterial lectin with activity against Gram-positive bacteria. Alternative splicing results in multiple transcript variants encoding multiple isoforms. [provided by RefSeq, Jun 2012]
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 UniProt Comments for REG3G    REG3G: Might be a stress protein involved in the control of bacterial proliferation.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 2p12

Cellular Component: cytoplasm; extracellular region

Molecular Function: carbohydrate binding

Biological Process: MyD88-dependent toll-like receptor signaling pathway; defense response to Gram-positive bacterium; negative regulation of keratinocyte differentiation; acute-phase response
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 Research Articles on REG3G    1. a model of CagA-directed REG3gamma expression in gastric epithelial cells via activation of the IL-11/gp130/STAT3 pathway
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with REG3G elisa kitDiseases associated with REG3G elisa kit
 Reference Product  PubMed Publications
 MUC1 elisa kit  >1 publications with REG3G and MUC1
 IL22 elisa kit  >1 publications with REG3G and IL22
 Disease Name  Pubmed Publications
 Inflammation Antibodies  >16 publications with REG3G and Inflammation
 Disease Models, Animal Antibodies  >7 publications with REG3G and Disease Models, Animal
 Necrosis Antibodies  >4 publications with REG3G and Necrosis
 Lung Diseases Antibodies  >3 publications with REG3G and Lung Diseases
 Immune System Diseases Antibodies  >2 publications with REG3G and Immune System Diseases
 Pneumonia Antibodies  >2 publications with REG3G and Pneumonia
 Carcinoma Antibodies  >2 publications with REG3G and Carcinoma
 Carcinoma, Hepatocellular Antibodies  >1 publications with REG3G and Carcinoma, Hepatocellular
 Fatty Liver Antibodies  >1 publications with REG3G and Fatty Liver
 Weight Loss Antibodies  >1 publications with REG3G and Weight Loss
Organs/Tissues associated with REG3G elisa kit
 Organ/Tissue Name  Pubmed Publications
 Intestine Antibodies  >29 publications with REG3G and Intestine
 Pancreas Antibodies  >6 publications with REG3G and Pancreas
 Stomach Antibodies  >2 publications with REG3G and Stomach
 Testis Antibodies  >1 publications with REG3G and Testis
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