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SDH elisa kit :: Bovine Sorbitol Dehydrogenase (SDH) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS2020264 (SPECIAL PROMOTION: Get FREE Starbucks gift card ($5 for 48-Wells-Kit or $10 for 96-Wells-Kit. Maximum 5 gift cards/product). Gift card(s) will be included with shipment)
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells  /  $420 +1 FREE 8GB USB
48-Strip-Wells  /  $630 +1 FREE 8GB USB
96-Strip-Wells  /  $860 +1 FREE 8GB USB
5x96-Strip-Wells  /  $3,535 +4 FREE 8GB USB
10x96-Strip-Wells  /  $6,410 +8 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Sorbitol Dehydrogenase (SDH), ELISA Kit

 Also Known As   

Sorbitol Dehydrogenase (SDH) ELISA Kit

 Product Synonym Names    SORD; L-iditol 2-dehydrogenase
 Product Gene Name   

SDH elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    182500
 3D Structure    ModBase 3D Structure for Q00796
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 Species Reactivity    Bovine
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 Specificity    This assay has high sensitivity and excellent specificity for detection of Sorbitol Dehydrogenase (SDH).
No significant cross-reactivity or interference between Sorbitol Dehydrogenase (SDH) and analogues was observed.
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 Samples    Serum, plasma, tissue homogenates and other biological fluids
 Assay Type    Sandwich
 Detection Range    3.13-200ng/mL
 Sensitivity    Typically less than 1.12ng/mL.
 Precision    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Sorbitol Dehydrogenase (SDH) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Sorbitol Dehydrogenase (SDH) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
 Assay Procedure Summary    1. Prepare all reagents, samples and standards;
2. Add 100uL standard or sample to each well. Incubate 2 hours at 37 degree C;
3. Aspirate and add 100uL prepared Detection Reagent A. Incubate 1 hour at 37 degree C;
4. Aspirate and wash 3 times;
5. Add 100uL prepared Detection Reagent B. Incubate 30 minutes at 37 degree C;
6. Aspirate and wash 5 times;
7. Add 90uL Substrate Solution. Incubate 15-25 minutes at 37 degree C;
8. Add 50uL Stop Solution. Read at 450nm immediately.
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 Preparation and Storage    For unopened kit: All the reagents should be kept according to the labels on vials. The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 degree C upon receipt while the others should be at 4 degree C.

For opened kit: When the kit is opened, the remaining reagents still need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and reseal along entire edge of zip-seal.

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
 Product Note    Our ELISA assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of SDH elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for SDH purchase    MBS2020264 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Sorbitol Dehydrogenase (SDH) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 3.13-200ng/mL in biological research samples containing SDH, with an estimated sensitivity of Typically less than 1.12ng/mL. The ELISA analytical biochemical technique of the MBS2020264 kit is based on SDH antibody-SDH antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect SDH antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, SDH. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, tissue homogenates and other biological fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Product Description specifically for SDH elisa kit

   Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Sorbitol Dehydrogenase (SDH). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Sorbitol Dehydrogenase (SDH). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Sorbitol Dehydrogenase (SDH), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Sorbitol Dehydrogenase (SDH) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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 Typical Testing Data/Standard Curve (for reference only) of SDH elisa kit    SDH elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS2020264. Click to request current manual
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NCBI/Uniprot data below describe general gene information for SDH. It may not necessarily be applicable to this product.
 NCBI GI #    156627571
 NCBI GeneID    6652
 NCBI Accession #    NP_003095.2 [Other Products]
 NCBI GenBank Nucleotide #    NM_003104.5 [Other Products]
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 UniProt Primary Accession #    Q00796 [Other Products]
 UniProt Secondary Accession #    Q16682; Q9UMD6; B2R655; B7Z3A6; J3JZZ5 [Other Products]
 UniProt Related Accession #    Q00796 [Other Products]
 Molecular Weight    10,812 Da
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 NCBI Official Full Name    sorbitol dehydrogenase
 NCBI Official Synonym Full Names    sorbitol dehydrogenase
 NCBI Official Symbol    SORD [Similar Products]
 NCBI Official Synonym Symbols   
SORD1; HEL-S-95n
[Similar Products]
 NCBI Protein Information    sorbitol dehydrogenase
 UniProt Protein Name    Sorbitol dehydrogenase
 UniProt Synonym Protein Names   
L-iditol 2-dehydrogenase
 Protein Family    Serine 3-dehydrogenase
 UniProt Gene Name    SORD [Similar Products]
 UniProt Entry Name    DHSO_HUMAN
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 NCBI Summary for SDH    Sorbitol dehydrogenase (SORD; EC 1.1.1.14) catalyzes the interconversion of polyols and their corresponding ketoses, and together with aldose reductase (ALDR1; MIM 103880), makes up the sorbitol pathway that is believed to play an important role in the development of diabetic complications (summarized by Carr and Markham, 1995 [PubMed 8535074]). The first reaction of the pathway (also called the polyol pathway) is the reduction of glucose to sorbitol by ALDR1 with NADPH as the cofactor. SORD then oxidizes the sorbitol to fructose using NAD(+) cofactor.[supplied by OMIM, Jul 2010]
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 UniProt Comments for SDH    SORD: Converts sorbitol to fructose. Part of the polyol pathway that plays an important role in sperm physiology. May play a role in the sperm motility by providing an energetic source for sperm. Belongs to the zinc-containing alcohol dehydrogenase family.

Protein type: EC 1.1.1.14; Carbohydrate Metabolism - fructose and mannose; Oxidoreductase

Chromosomal Location of Human Ortholog: 15q15.3

Cellular Component: extracellular space; membrane; mitochondrial membrane

Molecular Function: zinc ion binding; L-iditol 2-dehydrogenase activity; carbohydrate binding; NAD binding

Biological Process: sperm motility; L-xylitol metabolic process; L-xylitol catabolic process; glucose metabolic process; fructose biosynthetic process; sorbitol catabolic process
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 Research Articles on SDH    1. One of the most striking changes involved sorbitol dehydrogenase, a key enzyme in the polyol pathway. Validation studies revealed dramatically increased sorbitol dehydrogenase concentrations and activity in adenomas and cancer cell lines, along with important changes in the expression of other enzymes in the same (AKR1B1) and related (KHK) pathways.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with SDH elisa kitPathways associated with SDH elisa kit
 Reference Product  PubMed Publications
 HK1 elisa kit  >1 publications with SDH and HK1
 Products by Pathway  Pathway Diagram
 Fructose And Mannose Metabolism Pathway antibodies  Fructose And Mannose Metabolism Pathway Diagram
 Fructose And Mannose Metabolism Pathway antibodies  Fructose And Mannose Metabolism Pathway Diagram
 Glucuronate Pathway (uronate Pathway) antibodies  Glucuronate Pathway (uronate Pathway) Diagram
 Glucuronate Pathway (uronate Pathway) antibodies  Glucuronate Pathway (uronate Pathway) Diagram
 Metabolic Pathways antibodies  Metabolic Pathways Diagram
 Pentose And Glucuronate Interconversions Pathway antibodies  Pentose And Glucuronate Interconversions Pathway Diagram
 Pentose And Glucuronate Interconversions Pathway antibodies  Pentose And Glucuronate Interconversions Pathway Diagram
 Polyol Pathway antibodies  Polyol Pathway Diagram
 Sorbitol Degradation I Pathway antibodies  Sorbitol Degradation I Pathway Diagram
Diseases associated with SDH elisa kitOrgans/Tissues associated with SDH elisa kit
 Disease Name  Pubmed Publications
 Brain Diseases Antibodies  >3 publications with SDH and Brain Diseases
 Adenocarcinoma Antibodies  >2 publications with SDH and Adenocarcinoma
 Mental Disorders Antibodies  >2 publications with SDH and Mental Disorders
 Liver Diseases Antibodies  >2 publications with SDH and Liver Diseases
 Liver Cirrhosis Antibodies  >1 publications with SDH and Liver Cirrhosis
 Lung Neoplasms Antibodies  >1 publications with SDH and Lung Neoplasms
 Carcinoma, Hepatocellular Antibodies  >1 publications with SDH and Carcinoma, Hepatocellular
 Hyperglycemia Antibodies  >1 publications with SDH and Hyperglycemia
 Liver Neoplasms Antibodies  >1 publications with SDH and Liver Neoplasms
 Kidney Diseases Antibodies  >1 publications with SDH and Kidney Diseases
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >11 publications with SDH and Blood
 Brain Antibodies  >7 publications with SDH and Brain
 Eye Antibodies  >3 publications with SDH and Eye
 Kidney Antibodies  >3 publications with SDH and Kidney
 Liver Antibodies  >3 publications with SDH and Liver
 Vascular Antibodies  >2 publications with SDH and Vascular
 Muscle Antibodies  >2 publications with SDH and Muscle
 Larynx Antibodies  >1 publications with SDH and Larynx
 Testis Antibodies  >1 publications with SDH and Testis
 Thyroid Antibodies  >1 publications with SDH and Thyroid
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