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TGFbeta3 elisa kit :: Bovine Transforming Growth Factor beta3 ELISA Kit

Scan QR to view Datasheet Catalog #    MBS746373
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $455 +1 FREE 8GB USB
96-Strip-Wells  /  $660 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,925 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,490 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Transforming Growth Factor beta3 (TGFbeta3), ELISA Kit

★Popular Item★
 Also Known As   

Bovine Transforming Growth Factor beta3 ELISA Kit

 Product Synonym Names    Bovine Transforming Growth Factor b3 ELISA Kit
 Product Gene Name   

TGFbeta3 elisa kit

[Similar Products]
 Product Synonym Gene Name    TGFb3 [Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    gene 615582
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 Species Reactivity    Bovine
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 Specificity    This assay has high sensitivity and excellent specificity for detection of TGF-beta3. No significant cross-reactivity or interference between TGF-beta3 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between TGF-beta3 and all the analogues, therefore, cross reaction may still exist in some cases.
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 Samples    Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
 Assay Type    Competitive
 Sensitivity    1.0 pg/mL.
 Intended Uses    This TGF-beta3 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Bovine TGF-beta3. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
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 Preparation and Storage    Store all reagents at 2-8 degree C.
 Sample Preparation    We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of TGFbeta3 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for TGFbeta3 purchase    MBS746373 is a ready-to-use microwell, strip plate Competitive ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Transforming Growth Factor beta3 (TGFbeta3) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing TGFbeta3, with an estimated sensitivity of 1.0 pg/mL. The ELISA analytical biochemical technique of the MBS746373 kit is based on TGFbeta3 antibody-TGFbeta3 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect TGFbeta3 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, TGFbeta3. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for TGFbeta3 elisa kit

  
Principle of the assay: TGF-beta3 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-TGF-beta3 antibody and an TGF-beta3-HRP conjugate. The assay sample and buffer are incubated together with TGF-beta3-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the TGF-beta3 concentration since TGF-beta3 from samples and TGF-beta3-HRP conjugate compete for the anti-TGF-beta3 antibody binding site. Since the number of sites is limited, as more sites are occupied by TGF-beta3 from the sample, fewer sites are left to bind TGF-beta3-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The TGF-beta3 concentration in each sample is interpolated from this standard curve.
 Product Categories/Family for TGFbeta3 elisa kit    Cancer
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 Typical Testing Data/Standard Curve (for reference only) of TGFbeta3 elisa kit    TGFbeta3 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS746373. Click to request current manual
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NCBI/Uniprot data below describe general gene information for TGFbeta3. It may not necessarily be applicable to this product.
 NCBI GI #    4507465
 NCBI GeneID    7043
 NCBI Accession #    NP_003230.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_003239.3 [Other Products]
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 UniProt Related Accession #    P10600 [Other Products]
 Molecular Weight    47,328 Da
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 NCBI Official Full Name    transforming growth factor beta-3 preproprotein
 NCBI Official Synonym Full Names    transforming growth factor, beta 3
 NCBI Official Symbol    TGFB3 [Similar Products]
 NCBI Official Synonym Symbols   
ARVD; RNHF; ARVD1; TGF-beta3
[Similar Products]
 NCBI Protein Information    transforming growth factor beta-3; TGF-beta-3; prepro-transforming growth factor beta-3
 UniProt Protein Name    Transforming growth factor beta-3
 UniProt Gene Name    TGFB3 [Similar Products]
 UniProt Synonym Gene Names    TGF-beta-3; LAP [Similar Products]
 UniProt Entry Name    TGFB3_HUMAN
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 NCBI Summary for TGFbeta3    This gene encodes a member of the TGF-beta family of proteins. The encoded protein is secreted and is involved in embryogenesis and cell differentiation. Defects in this gene are a cause of familial arrhythmogenic right ventricular dysplasia 1. [provided by RefSeq, Mar 2009]
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 UniProt Comments for TGFbeta3    TGFB3: Involved in embryogenesis and cell differentiation. Homodimer; disulfide-linked. Interacts with ASPN. Belongs to the TGF-beta family.

Protein type: Motility/polarity/chemotaxis; Ligand, receptor tyrosine kinase; Secreted; Cell development/differentiation; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 14q24

Cellular Component: extracellular matrix; extracellular space; cell surface; cell soma; T-tubule; plasma membrane; extracellular region; nucleus

Molecular Function: identical protein binding; protein binding; growth factor activity; transforming growth factor beta binding; protein heterodimerization activity; punt binding; cytokine activity

Biological Process: extracellular matrix organization and biogenesis; activation of MAPK activity; positive regulation of apoptosis; positive regulation of transcription, DNA-dependent; positive regulation of collagen biosynthetic process; palate development; female pregnancy; SMAD protein nuclear translocation; regulation of apoptosis; odontogenesis; negative regulation of cell proliferation; platelet degranulation; mammary gland development; transforming growth factor beta receptor signaling pathway; salivary gland morphogenesis; embryonic neurocranium morphogenesis; negative regulation of neuron apoptosis; cell growth; inner ear development; aging; uterine wall breakdown; positive regulation of filopodium formation; platelet activation; intercellular junction assembly and maintenance; in utero embryonic development; positive regulation of bone mineralization; regulation of cell proliferation; positive regulation of protein secretion; gut development; response to estrogen stimulus; negative regulation of DNA replication; positive regulation of cell division; regulation of MAPKKK cascade; response to hypoxia; positive regulation of transcription from RNA polymerase II promoter; blood coagulation; response to progesterone stimulus; negative regulation of transforming growth factor beta receptor signaling pathway; alveolus development; positive regulation of DNA replication

Disease: Arrhythmogenic Right Ventricular Dysplasia, Familial, 1; Rienhoff Syndrome
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 Research Articles on TGFbeta3    1. TGF-beta3 could be stably expressed in pcDNA3.1(+)-hTGF-beta3-transfected PSCs.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with TGFbeta3 elisa kitPathways associated with TGFbeta3 elisa kit
 Reference Product  PubMed Publications
 TGFB2 elisa kit  >567 publications with TGFbeta3 and TGFB2
 EGF elisa kit  >21 publications with TGFbeta3 and EGF
 SERPINE1 elisa kit  >16 publications with TGFbeta3 and SERPINE1
 TGFBR1 elisa kit  >9 publications with TGFbeta3 and TGFBR1
 TGFBR2 elisa kit  >9 publications with TGFbeta3 and TGFBR2
 SMAD5 elisa kit  >2 publications with TGFbeta3 and SMAD5
 TIMP1 elisa kit  >1 publications with TGFbeta3 and TIMP1
 ACVRL1 elisa kit  >1 publications with TGFbeta3 and ACVRL1
 FN1 elisa kit  >1 publications with TGFbeta3 and FN1
 Products by Pathway  Pathway Diagram
 ALK1 Signaling Events Pathway antibodies  ALK1 Signaling Events Pathway Diagram
 Amoebiasis Pathway antibodies  Amoebiasis Pathway Diagram
 Amoebiasis Pathway antibodies  Amoebiasis Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
 Chagas Disease (American Trypanosomiasis) Pathway antibodies  Chagas Disease (American Trypanosomiasis) Pathway Diagram
 Chagas Disease (American Trypanosomiasis) Pathway antibodies  Chagas Disease (American Trypanosomiasis) Pathway Diagram
 Chronic Myeloid Leukemia Pathway antibodies  Chronic Myeloid Leukemia Pathway Diagram
 Chronic Myeloid Leukemia Pathway antibodies  Chronic Myeloid Leukemia Pathway Diagram
 Colorectal Cancer Pathway antibodies  Colorectal Cancer Pathway Diagram
Diseases associated with TGFbeta3 elisa kitOrgans/Tissues associated with TGFbeta3 elisa kit
 Disease Name  Pubmed Publications
 Fibrosis Antibodies  >188 publications with TGFbeta3 and Fibrosis
 Disease Models, Animal Antibodies  >103 publications with TGFbeta3 and Disease Models, Animal
 Cleft Palate Antibodies  >103 publications with TGFbeta3 and Cleft Palate
 Cardiovascular Diseases Antibodies  >87 publications with TGFbeta3 and Cardiovascular Diseases
 Inflammation Antibodies  >81 publications with TGFbeta3 and Inflammation
 Necrosis Antibodies  >64 publications with TGFbeta3 and Necrosis
 Heart Diseases Antibodies  >53 publications with TGFbeta3 and Heart Diseases
 Hypertrophy Antibodies  >38 publications with TGFbeta3 and Hypertrophy
 Breast Neoplasms Antibodies  >38 publications with TGFbeta3 and Breast Neoplasms
 Lung Diseases Antibodies  >36 publications with TGFbeta3 and Lung Diseases
 Organ/Tissue Name  Pubmed Publications
 Bone Antibodies  >351 publications with TGFbeta3 and Bone
 Connective Tissue Antibodies  >242 publications with TGFbeta3 and Connective Tissue
 Embryonic Tissue Antibodies  >143 publications with TGFbeta3 and Embryonic Tissue
 Bone Marrow Antibodies  >122 publications with TGFbeta3 and Bone Marrow
 Mouth Antibodies  >119 publications with TGFbeta3 and Mouth
 Heart Antibodies  >79 publications with TGFbeta3 and Heart
 Lung Antibodies  >76 publications with TGFbeta3 and Lung
 Eye Antibodies  >66 publications with TGFbeta3 and Eye
 Brain Antibodies  >64 publications with TGFbeta3 and Brain
 Liver Antibodies  >51 publications with TGFbeta3 and Liver
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