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VEGFA elisa kit :: Bovine Vascular endothelial growth factor A ELISA Kit

Scan QR to view Datasheet Catalog #    MBS2887434
Typical Standard Curve/Testing Data
Unit / Price
48-Strip-Wells  /  $325 +1 FREE 8GB USB
96-Strip-Wells  /  $435 +1 FREE 8GB USB
5x96-Strip-Wells  /  $1,825 +2 FREE 8GB USB
10x96-Strip-Wells  /  $3,290 +4 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Vascular endothelial growth factor A (VEGFA), ELISA Kit

★Popular Item★
 Also Known As   

Bovine Vascular endothelial growth factor A ELISA Kit

 Product Synonym Names    Vascular endothelial growth factor A; VEGF-A; Vascular permeability factor; VPF; VEGFA; VEGF
 Product Gene Name   

VEGFA elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS2887434 Testing Data    Testing Data PDF
 3D Structure    ModBase 3D Structure for P15691
 Species Reactivity    Bovine
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 Samples    Serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
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 Preparation and Storage    For long term storage, please store the entire kit at -20 degree C.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of VEGFA elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for VEGFA purchase    MBS2887434 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Vascular endothelial growth factor A (VEGFA) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing VEGFA. The ELISA analytical biochemical technique of the MBS2887434 kit is based on VEGFA antibody-VEGFA antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect VEGFA antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, VEGFA. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, tissue homogenates, cell culture supernates or other biological fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for VEGFA elisa kit

   Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of target antigen concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to target antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for target antigen and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain target antigen, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of target antigen in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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 Typical Standard Curve/Testing Data of VEGFA elisa kit    VEGFA elisa kit Typical Standard Curve/Testing Data image
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Sample Manual Insert of MBS2887434. Click to request current manual
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NCBI/Uniprot data below describe general gene information for VEGFA. It may not necessarily be applicable to this product.
 NCBI GI #    27806357
 NCBI GeneID    281572
 NCBI Accession #    NP_776641.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_174216.2 [Other Products]
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 UniProt Primary Accession #    P15691 [Other Products]
 UniProt Related Accession #    P15691 [Other Products]
 Molecular Weight    17,086 Da
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 NCBI Official Full Name    vascular endothelial growth factor A isoform VEGF-A
 NCBI Official Symbol    VEGFA [Similar Products]
 NCBI Official Synonym Symbols   
VPF; VEGF; VEGF-A; eVEGF120; eVEGF164
[Similar Products]
 NCBI Protein Information    vascular endothelial growth factor A
 UniProt Protein Name    Vascular endothelial growth factor A
 UniProt Synonym Protein Names   
Vascular permeability factor; VPF
 Protein Family    Vascular endothelial growth factor
 UniProt Gene Name    VEGFA [Similar Products]
 UniProt Synonym Gene Names    VEGF; VEGF-A; VPF [Similar Products]
 UniProt Entry Name    VEGFA_BOVIN
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 NCBI Summary for VEGFA    This gene is a member of the PDGF/VEGF growth factor family. It encodes a heparin-binding protein, which exists as a disulfide-linked homodimer. This growth factor induces proliferation and migration of vascular endothelial cells, and is essential for both physiological and pathological angiogenesis. It is upregulated in many known tumors and its expression is correlated with tumor stage and progression. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. A recent study showed that a C-terminally extended isoform is produced by use of an alternative in-frame translation termination codon via a stop codon readthrough mechanism, and that this isoform is antiangiogenic. [provided by RefSeq, Nov 2015]
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 UniProt Comments for VEGFA    Growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. Binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin ().
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 Research Articles on VEGFA    1. Studied the effect of cyclic uniaxial stretch (20%, 1 Hz), with and without the stimulation of vascular endothelial growth factor (VEGF), on sprouting angiogenesis by employing a stretchable three-dimensional cell culture model.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with VEGFA elisa kitPathways associated with VEGFA elisa kit
 Reference Product  PubMed Publications
 HIF1A elisa kit  >610 publications with VEGFA and HIF1A
 KDR elisa kit  >291 publications with VEGFA and KDR
 FLT1 elisa kit  >197 publications with VEGFA and FLT1
 KIT elisa kit  >88 publications with VEGFA and KIT
 NRP1 elisa kit  >59 publications with VEGFA and NRP1
 ARNT elisa kit  >17 publications with VEGFA and ARNT
 EPAS1 elisa kit  >12 publications with VEGFA and EPAS1
 HIF3A elisa kit  >9 publications with VEGFA and HIF3A
 SHC1 elisa kit  >3 publications with VEGFA and SHC1
 Products by Pathway  Pathway Diagram
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 AGE-RAGE Signaling Pathway In Diabetic Complications antibodies  AGE-RAGE Signaling Pathway In Diabetic Complications Diagram
 Bladder Cancer Pathway antibodies  Bladder Cancer Pathway Diagram
 Bladder Cancer Pathway antibodies  Bladder Cancer Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 Endochondral Ossification Pathway antibodies  Endochondral Ossification Pathway Diagram
 Focal Adhesion Pathway antibodies  Focal Adhesion Pathway Diagram
 Focal Adhesion Pathway antibodies  Focal Adhesion Pathway Diagram
 HIF-1 Signaling Pathway antibodies  HIF-1 Signaling Pathway Diagram
Diseases associated with VEGFA elisa kitOrgans/Tissues associated with VEGFA elisa kit
 Disease Name  Pubmed Publications
 Neovascularization, Pathologic Antibodies  >2145 publications with VEGFA and Neovascularization, Pathologic
 Retinal Diseases Antibodies  >1717 publications with VEGFA and Retinal Diseases
 Neoplasm Metastasis Antibodies  >549 publications with VEGFA and Neoplasm Metastasis
 Diabetic Retinopathy Antibodies  >534 publications with VEGFA and Diabetic Retinopathy
 Inflammation Antibodies  >531 publications with VEGFA and Inflammation
 Diabetes Complications Antibodies  >458 publications with VEGFA and Diabetes Complications
 Lung Diseases Antibodies  >450 publications with VEGFA and Lung Diseases
 Macular Edema Antibodies  >433 publications with VEGFA and Macular Edema
 Heart Diseases Antibodies  >307 publications with VEGFA and Heart Diseases
 Head and Neck Neoplasms Antibodies  >290 publications with VEGFA and Head and Neck Neoplasms
 Organ/Tissue Name  Pubmed Publications
 Muscle Antibodies  >490 publications with VEGFA and Muscle
 Liver Antibodies  >387 publications with VEGFA and Liver
 Kidney Antibodies  >373 publications with VEGFA and Kidney
 Brain Antibodies  >356 publications with VEGFA and Brain
 Skin Antibodies  >300 publications with VEGFA and Skin
 Uterus Antibodies  >127 publications with VEGFA and Uterus
 Intestine Antibodies  >58 publications with VEGFA and Intestine
 Pancreas Antibodies  >54 publications with VEGFA and Pancreas
 Extraembryonic Tissue Antibodies  >6 publications with VEGFA and Extraembryonic Tissue
 Uncharacterized Tissue Antibodies  >2 publications with VEGFA and Uncharacterized Tissue
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