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ACHE elisa kit :: Bovine acetylcholinesterase (Yt blood group) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS908990
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells (LIMIT 1)  /  $250 +1 FREE 8GB USB
48-Strip-Wells  /  $550 +1 FREE 8GB USB
96-Strip-Wells  /  $795 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,890 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,465 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

acetylcholinesterase (Yt blood group) (ACHE), ELISA Kit

 Also Known As   

Bovine Acetylcholinesterase, ACHE ELISA Kit

 Product Synonym Names    Bovine Acetylcholinesterase (ACHE) ELISA kit; ARACHE; N-ACHE; YT; OTTHUMP00000211347; OTTHUMP00000211349; OTTHUMP00000211356; acetylcholinesterase; apoptosis-related acetylcholinesterase; acetylcholinesterase (Yt blood group)
 Product Gene Name   

ACHE elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS908990 Testing Data    Testing Data PDF
 3D Structure    ModBase 3D Structure for P23795
 Species Reactivity    Bovine
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 Specificity    This assay has high sensitivity and excellent specificity for detection of bovine ACHE. No significant cross-reactivity or interference between bovine ACHE and analogues was observed.
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 Samples    Serum, plasma
 Assay Type    Sandwich
 Detection Range    0.9 4 U/ml -60 U/ml.
 Sensitivity    The minimum detectable dose of bovine ACHE is typically less than 0.24 U/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
 Inter-assay Precision    Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
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 Preparation and Storage    Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of ACHE elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for ACHE purchase    MBS908990 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the acetylcholinesterase (Yt blood group) (ACHE) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 0.9 4 U/ml -60 U/ml in biological research samples containing ACHE, with an estimated sensitivity of The minimum detectable dose of bovine ACHE is typically less than 0.24 U/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations. The ELISA analytical biochemical technique of the MBS908990 kit is based on ACHE antibody-ACHE antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect ACHE antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, ACHE. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for ACHE elisa kit

   Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for ACHE has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ACHE present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ACHE is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ACHE bound in the initial step. The color development is stopped and the intensity of the color is measured.
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 Typical Testing Data/Standard Curve (for reference only) of ACHE elisa kit    ACHE elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS908990. Click to request current manual
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NCBI/Uniprot data below describe general gene information for ACHE. It may not necessarily be applicable to this product.
 NCBI GI #    115497516
 NCBI GeneID    540446
 NCBI Accession #    NP_001069688.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001076220.1 [Other Products]
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 UniProt Primary Accession #    P23795 [Other Products]
 UniProt Secondary Accession #    O97579; Q08D79 [Other Products]
 UniProt Related Accession #    P23795 [Other Products]
 Molecular Weight    67,664 Da
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 NCBI Official Full Name    acetylcholinesterase
 NCBI Official Synonym Full Names    acetylcholinesterase
 NCBI Official Symbol    ACHE [Similar Products]
 NCBI Protein Information    acetylcholinesterase; acetylcholinesterase (Yt blood group)
 UniProt Protein Name    Acetylcholinesterase
 UniProt Gene Name    ACHE [Similar Products]
 UniProt Synonym Gene Names    AChE [Similar Products]
 UniProt Entry Name    ACES_BOVIN
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 UniProt Comments for ACHE    Function: Terminates signal transduction at the neuromuscular junction by rapid hydrolysis of the acetylcholine released into the synaptic cleft.

Catalytic activity: Acetylcholine + H2O = choline + acetate.
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 Research Articles on ACHE    1. To date, AChE and BChE are the only proteins known that include polyproline tetramer organizing peptides in their tetrameric structure.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Pathways associated with ACHE elisa kitDiseases associated with ACHE elisa kit
 Products by Pathway  Pathway Diagram
 Acetylcholine Synthesis Pathway antibodies  Acetylcholine Synthesis Pathway Diagram
 Cholinergic Synapse Pathway antibodies  Cholinergic Synapse Pathway Diagram
 Glycerophospholipid Metabolism Pathway antibodies  Glycerophospholipid Metabolism Pathway Diagram
 Glycerophospholipid Metabolism Pathway antibodies  Glycerophospholipid Metabolism Pathway Diagram
 Neuronal System Pathway antibodies  Neuronal System Pathway Diagram
 Neurotransmitter Clearance In The Synaptic Cleft Pathway antibodies  Neurotransmitter Clearance In The Synaptic Cleft Pathway Diagram
 Transmission Across Chemical Synapses Pathway antibodies  Transmission Across Chemical Synapses Pathway Diagram
 Disease Name  Pubmed Publications
 Pain Antibodies  >499109 publications with ACHE and Pain
 Nervous System Diseases Antibodies  >205592 publications with ACHE and Nervous System Diseases
 Heart Diseases Antibodies  >59919 publications with ACHE and Heart Diseases
 Neoplasms Antibodies  >57669 publications with ACHE and Neoplasms
 Myocardial Ischemia Antibodies  >53555 publications with ACHE and Myocardial Ischemia
 Gastrointestinal Diseases Antibodies  >34394 publications with ACHE and Gastrointestinal Diseases
 Central Nervous System Diseases Antibodies  >33537 publications with ACHE and Central Nervous System Diseases
 Headache Antibodies  >29864 publications with ACHE and Headache
 Peripheral Nervous System Diseases Antibodies  >25869 publications with ACHE and Peripheral Nervous System Diseases
 Brain Diseases Antibodies  >25245 publications with ACHE and Brain Diseases
Organs/Tissues associated with ACHE elisa kit
 Organ/Tissue Name  Pubmed Publications
 Muscle Antibodies  >35015 publications with ACHE and Muscle
 Brain Antibodies  >28865 publications with ACHE and Brain
 Skin Antibodies  >18655 publications with ACHE and Skin
 Liver Antibodies  >11306 publications with ACHE and Liver
 Kidney Antibodies  >11038 publications with ACHE and Kidney
 Intestine Antibodies  >10439 publications with ACHE and Intestine
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