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BGLAP elisa kit :: Bovine bone gamma-carboxyglutamate (gla) protein ELISA Kit

Scan QR to view Datasheet Catalog #    MBS703264
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells (LIMIT 1)  /  $250 +1 FREE 8GB USB
48-Strip-Wells  /  $550 +1 FREE 8GB USB
96-Strip-Wells  /  $795 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,890 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,465 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

bone gamma-carboxyglutamate (gla) protein (BGLAP), ELISA Kit

 Also Known As   

Bovine Osteocalcin/bone gla protein, OT/BGP ELISA Kit

 Product Synonym Names    Bovine Osteocalcin/bone gla protein; OT/BGP ELISA Kit; RP11-54H19.5; BGP; OC; PMF1; bone Gla protein; bone gamma-carboxyglutamate (gla) protein (osteocalcin) ; gamma-carboxyglutamic acid-containing protein; osteocalcin; bone gamma-carboxyglutamate (gla) protein
 Product Gene Name   

BGLAP elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS703264 Testing Data    Testing Data PDF
 3D Structure    ModBase 3D Structure for P02820
 Species Reactivity    Bovine
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 Specificity    This assay has high sensitivity and excellent specificity for detection of Bovine BGLAP. No significant cross-reactivity or interference between Bovine BGLAP and analogues was observed.
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 Samples    Serum, plasma, tissue homogenates
 Detection Range    2.5 ng/ml-160 ng/ml
 Sensitivity    0.625 ng/ml
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): CV% is less than 8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV% is less than 10%
Three samples of known concentration were tested in twenty assays to assess.
 Detection Wavelength    450 nm
 Sample Volume    50-100ul
 Protein Biological Process 1    Biosynthesis/Metabolism
 Protein Biological Process 3    Biomineralization
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 Preparation and Storage    Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of BGLAP elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for BGLAP purchase    MBS703264 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the bone gamma-carboxyglutamate (gla) protein (BGLAP) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 2.5 ng/ml-160 ng/ml in biological research samples containing BGLAP, with an estimated sensitivity of 0.625 ng/ml. The ELISA analytical biochemical technique of the MBS703264 kit is based on BGLAP antibody-BGLAP antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect BGLAP antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, BGLAP. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, tissue homogenates. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for BGLAP elisa kit

   Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to BGP. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for BGP and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain BGP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of BGP in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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 Typical Testing Data/Standard Curve (for reference only) of BGLAP elisa kit    BGLAP elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS703264. Click to request current manual
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NCBI/Uniprot data below describe general gene information for BGLAP. It may not necessarily be applicable to this product.
 NCBI GI #    27806301
 NCBI GeneID    281646
 NCBI Accession #    NP_776674.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_174249.2 [Other Products]
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 UniProt Primary Accession #    P02820 [Other Products]
 UniProt Secondary Accession #    A7YEW1 [Other Products]
 UniProt Related Accession #    P02820 [Other Products]
 Molecular Weight    11,042 Da
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 NCBI Official Full Name    osteocalcin preproprotein
 NCBI Official Synonym Full Names    bone gamma-carboxyglutamate (gla) protein
 NCBI Official Symbol    BGLAP [Similar Products]
 NCBI Official Synonym Symbols   
BGP
[Similar Products]
 NCBI Protein Information    osteocalcin; bone Gla protein; gamma-carboxyglutamic acid-containing protein
 UniProt Protein Name    Osteocalcin
 UniProt Synonym Protein Names   
Bone Gla protein; BGP; Gamma-carboxyglutamic acid-containing protein
 Protein Family    Osteocalcin
 UniProt Gene Name    BGLAP [Similar Products]
 UniProt Synonym Gene Names    BGP [Similar Products]
 UniProt Entry Name    OSTCN_BOVIN
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 UniProt Comments for BGLAP    Function: Constitutes 1-2% of the total bone protein. It binds strongly to apatite and calcium.
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 Research Articles on BGLAP    1. Data suggest that helical content of 3 Glu-OCN is 57% and is independent of calcium binding; 3 Glu-OCN contains 3 alpha-helices around a hydrophobic core; study includes first high-resolution structure of bovine 3 Glu-OCN using X-ray crystallography.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with BGLAP elisa kitPathways associated with BGLAP elisa kit
 Reference Product  PubMed Publications
 SPP1 elisa kit  >19 publications with BGLAP and SPP1
 BMP2 elisa kit  >12 publications with BGLAP and BMP2
 PTH elisa kit  >2 publications with BGLAP and PTH
 LEP elisa kit  >1 publications with BGLAP and LEP
 Products by Pathway  Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
 Cell Cycle, Mitotic Pathway antibodies  Cell Cycle, Mitotic Pathway Diagram
 DNA Replication Pathway antibodies  DNA Replication Pathway Diagram
 FGF Signaling Pathway antibodies  FGF Signaling Pathway Diagram
 Glucocorticoid Receptor Regulatory Network Pathway antibodies  Glucocorticoid Receptor Regulatory Network Pathway Diagram
 M Phase Pathway antibodies  M Phase Pathway Diagram
 Mitotic M-M/G1 Phases Pathway antibodies  Mitotic M-M/G1 Phases Pathway Diagram
 Mitotic Prometaphase Pathway antibodies  Mitotic Prometaphase Pathway Diagram
 Notch-mediated HES/HEY Network Pathway antibodies  Notch-mediated HES/HEY Network Pathway Diagram
 Regulation Of Retinoblastoma Protein Pathway antibodies  Regulation Of Retinoblastoma Protein Pathway Diagram
Diseases associated with BGLAP elisa kitOrgans/Tissues associated with BGLAP elisa kit
 Disease Name  Pubmed Publications
 Bone Diseases Antibodies  >9 publications with BGLAP and Bone Diseases
 Osteoporosis Antibodies  >8 publications with BGLAP and Osteoporosis
 Nervous System Diseases Antibodies  >4 publications with BGLAP and Nervous System Diseases
 Inflammation Antibodies  >2 publications with BGLAP and Inflammation
 Pain Antibodies  >1 publications with BGLAP and Pain
 Weight Loss Antibodies  >1 publications with BGLAP and Weight Loss
 Kidney Diseases Antibodies  >1 publications with BGLAP and Kidney Diseases
 Hypertension Antibodies  >1 publications with BGLAP and Hypertension
 Cardiovascular Diseases Antibodies  >1 publications with BGLAP and Cardiovascular Diseases
 Necrosis Antibodies  >1 publications with BGLAP and Necrosis
 Organ/Tissue Name  Pubmed Publications
 Bone Antibodies  >31 publications with BGLAP and Bone
 Brain Antibodies  >2 publications with BGLAP and Brain
 Parathyroid Antibodies  >1 publications with BGLAP and Parathyroid
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