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CDH1 elisa kit :: Bovine cadherin 1, type 1, E-cadherin (epithelial) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS938537
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells (Limit 1)  /  $250 +1 FREE 8GB USB
48-Strip-Wells  /  $550 +1 FREE 8GB USB
96-Strip-Wells  /  $795 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,890 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,465 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

cadherin 1, type 1, E-cadherin (epithelial) (CDH1), ELISA Kit

★Popular Item★
 Also Known As   

Bovine Cadherin-1, CDH1 ELISA Kit

 Product Synonym Names    Bovine Cadherin-1 (CDH1) ELISA kit; Arc-1; CD324; CDHE; ECAD; LCAM; UVO; E-Cadherin; cadherin 1; E-cadherin (epithelial) ; cadherin 1; type 1; calcium-dependent adhesion protein; epithelial; cell-CAM 120/80; uvomorulin; cadherin 1; type 1; E-cadherin (epithelial)
 Product Gene Name   

CDH1 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS938537 Testing Data    Testing Data PDF
 3D Structure    ModBase 3D Structure for Q6R8F2
 Species Reactivity    Bovine
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 Specificity    This assay has high sensitivity and excellent specificity for detection of bovine CDH1. No significant cross-reactivity or interference between bovine CDH1 and analogues was observed.
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 Samples    Serum, plasma, tissue homogenates
 Assay Type    Sandwich
 Detection Range    7.8 ng/ml -500 ng/ml.
 Sensitivity    The minimum detectable dose of bovine CDH1 is typically less than 1.95 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
 Inter-assay Precision    Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
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 Preparation and Storage    Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of CDH1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for CDH1 purchase    MBS938537 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the cadherin 1, type 1, E-cadherin (epithelial) (CDH1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 7.8 ng/ml -500 ng/ml in biological research samples containing CDH1, with an estimated sensitivity of The minimum detectable dose of bovine CDH1 is typically less than 1.95 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations. The ELISA analytical biochemical technique of the MBS938537 kit is based on CDH1 antibody-CDH1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect CDH1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, CDH1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, tissue homogenates. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for CDH1 elisa kit

   Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for CDH1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CDH1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CDH1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CDH1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
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 Typical Testing Data/Standard Curve (for reference only) of CDH1 elisa kit    CDH1 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS938537. Click to request current manual
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NCBI/Uniprot data below describe general gene information for CDH1. It may not necessarily be applicable to this product.
 NCBI GI #    50582555
 NCBI GeneID    282637
 NCBI Accession #    NP_001002763.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001002763.1 [Other Products]
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 UniProt Primary Accession #    Q6R8F2 [Other Products]
 UniProt Related Accession #    Q6R8F2 [Other Products]
 Molecular Weight    97,938 Da
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 NCBI Official Full Name    cadherin-1
 NCBI Official Synonym Full Names    cadherin 1, type 1, E-cadherin (epithelial)
 NCBI Official Symbol    CDH1 [Similar Products]
 NCBI Protein Information    cadherin-1; E-cadherin; epithelial cadherin
 UniProt Protein Name    Cadherin-1
 UniProt Synonym Protein Names   
Epithelial cadherin; E-cadherin; CD_antigen: CD324Cleaved into the following 3 chains:E-Cad/CTF1; E-Cad/CTF2; E-Cad/CTF3
 Protein Family    Cadherin
 UniProt Gene Name    CDH1 [Similar Products]
 UniProt Synonym Gene Names    E-cadherin [Similar Products]
 UniProt Entry Name    CADH1_BOVIN
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 UniProt Comments for CDH1    Function: Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. CDH1 is involved in mechanisms regulating cell-cell adhesions, mobility and proliferation of epithelial cells. Has a potent invasive suppressor role. It is a ligand for integrin alpha-E/beta-7
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 Research Articles on CDH1    1. Transfection of zygotes with 100 and 200 nM E-cadherin siRNA led to a 72 and 38% reduction, respectively, in E-cadherin mRNA relative abundance in Day 7 blastocysts compared with controls.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Pathways associated with CDH1 elisa kitDiseases associated with CDH1 elisa kit
 Products by Pathway  Pathway Diagram
 Adaptive Immune System Pathway antibodies  Adaptive Immune System Pathway Diagram
 Adherens Junction Pathway antibodies  Adherens Junction Pathway Diagram
 Adherens Junction Pathway antibodies  Adherens Junction Pathway Diagram
 Adherens Junctions Interactions Pathway antibodies  Adherens Junctions Interactions Pathway Diagram
 Apoptosis Pathway antibodies  Apoptosis Pathway Diagram
 Apoptotic Cleavage Of Cell Adhesion Proteins Pathway antibodies  Apoptotic Cleavage Of Cell Adhesion Proteins Pathway Diagram
 Apoptotic Cleavage Of Cellular Proteins Pathway antibodies  Apoptotic Cleavage Of Cellular Proteins Pathway Diagram
 Apoptotic Execution Phase Pathway antibodies  Apoptotic Execution Phase Pathway Diagram
 Bacterial Invasion Of Epithelial Cells Pathway antibodies  Bacterial Invasion Of Epithelial Cells Pathway Diagram
 Bacterial Invasion Of Epithelial Cells Pathway antibodies  Bacterial Invasion Of Epithelial Cells Pathway Diagram
 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >673 publications with CDH1 and Neoplasms
 Carcinoma Antibodies  >323 publications with CDH1 and Carcinoma
 Gastrointestinal Diseases Antibodies  >312 publications with CDH1 and Gastrointestinal Diseases
 Stomach Neoplasms Antibodies  >225 publications with CDH1 and Stomach Neoplasms
 Adenocarcinoma Antibodies  >191 publications with CDH1 and Adenocarcinoma
 Breast Neoplasms Antibodies  >164 publications with CDH1 and Breast Neoplasms
 Colorectal Neoplasms Antibodies  >74 publications with CDH1 and Colorectal Neoplasms
 Neoplasm Metastasis Antibodies  >71 publications with CDH1 and Neoplasm Metastasis
 Neoplasm Invasiveness Antibodies  >69 publications with CDH1 and Neoplasm Invasiveness
 Carcinoma, Lobular Antibodies  >42 publications with CDH1 and Carcinoma, Lobular
Organs/Tissues associated with CDH1 elisa kit
 Organ/Tissue Name  Pubmed Publications
 Liver Antibodies  >48 publications with CDH1 and Liver
 Brain Antibodies  >42 publications with CDH1 and Brain
 Lung Antibodies  >40 publications with CDH1 and Lung
 Ovary Antibodies  >14 publications with CDH1 and Ovary
 Skin Antibodies  >13 publications with CDH1 and Skin
 Muscle Antibodies  >12 publications with CDH1 and Muscle
 Intestine Antibodies  >10 publications with CDH1 and Intestine
 Uterus Antibodies  >9 publications with CDH1 and Uterus
 Pancreas Antibodies  >8 publications with CDH1 and Pancreas
 Reticulum Antibodies  >5 publications with CDH1 and Reticulum
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