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CCL20 elisa kit :: Bovine chemokine (C-C motif) ligand 20 ELISA Kit

Scan QR to view Datasheet Catalog #    MBS934740
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells (LIMIT 1)  /  $250 +1 FREE 8GB USB
48-Strip-Wells  /  $550 +1 FREE 8GB USB
96-Strip-Wells  /  $795 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,890 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,465 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

chemokine (C-C motif) ligand 20 (CCL20), ELISA Kit

★Popular Item★
 Also Known As   

Bovine C-C motif chemokine 20, CCL20 ELISA Kit

 Product Synonym Names    Bovine C-C motif chemokine 20 (CCL20) ELISA kit; CKb4; LARC; MIP-3a; MIP3A; SCYA20; ST38; exodus-1; macrophage inflammatory protein 3 alpha; small inducible cytokine subfamily A (Cys-Cys); member 20; chemokine (C-C motif) ligand 20
 Product Gene Name   

CCL20 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS934740 Testing Data    Testing Data PDF
 3D Structure    ModBase 3D Structure for Q8SQB1
 Species Reactivity    Bovine
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 Specificity    This assay has high sensitivity and excellent specificity for detection of bovine CCL20. No significant cross-reactivity or interference between bovine CCL20 and analogues was observed.
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 Samples    Serum, plasma and tissue homogenates
 Assay Type    Sandwich
 Detection Range    15.6 pg/ml -1000 pg/ml.
 Sensitivity    The minimum detectable dose of bovine CCL20 is typically less than 3.9 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
 Inter-assay Precision    Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
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 Preparation and Storage    Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of CCL20 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for CCL20 purchase    MBS934740 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the chemokine (C-C motif) ligand 20 (CCL20) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 15.6 pg/ml -1000 pg/ml in biological research samples containing CCL20, with an estimated sensitivity of The minimum detectable dose of bovine CCL20 is typically less than 3.9 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations. The ELISA analytical biochemical technique of the MBS934740 kit is based on CCL20 antibody-CCL20 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect CCL20 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, CCL20. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma and tissue homogenates. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for CCL20 elisa kit

   Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for CCL20 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CCL20 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CCL20 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CCL20 bound in the initial step. The color development is stopped and the intensity of the color is measured.
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 Typical Testing Data/Standard Curve (for reference only) of CCL20 elisa kit    CCL20 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS934740. Click to request current manual
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NCBI/Uniprot data below describe general gene information for CCL20. It may not necessarily be applicable to this product.
 NCBI GI #    27806273
 NCBI GeneID    281666
 NCBI Accession #    NP_776688.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_174263.2 [Other Products]
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 UniProt Primary Accession #    Q8SQB1 [Other Products]
 UniProt Related Accession #    Q8SQB1 [Other Products]
 Molecular Weight    10,964 Da
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 NCBI Official Full Name    C-C motif chemokine 20
 NCBI Official Synonym Full Names    chemokine (C-C motif) ligand 20
 NCBI Official Symbol    CCL20 [Similar Products]
 NCBI Protein Information    C-C motif chemokine 20; MIP-3-alpha; small-inducible cytokine A20; macrophage inflammatory protein 3 alpha
 UniProt Protein Name    C-C motif chemokine 20
 UniProt Synonym Protein Names   
Macrophage inflammatory protein 3 alpha; MIP-3-alpha; Small-inducible cytokine A20
 Protein Family    C-C motif chemokine
 UniProt Gene Name    CCL20 [Similar Products]
 UniProt Synonym Gene Names    MIP3A; SCYA20; MIP-3-alpha [Similar Products]
 UniProt Entry Name    CCL20_BOVIN
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 UniProt Comments for CCL20    Function: Chemotactic factor that attracts lymphocytes and, slightly, neutrophils, but not monocytes. Inhibits proliferation of myeloid progenitors in colony formation assays. May be involved in formation and function of the mucosal lymphoid tissues by attracting lymphocytes and dendritic cells towards epithelial cells
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Pathways associated with CCL20 elisa kitDiseases associated with CCL20 elisa kit
 Products by Pathway  Pathway Diagram
 Chemokine Receptors Bind Chemokines Pathway antibodies  Chemokine Receptors Bind Chemokines Pathway Diagram
 Chemokine Signaling Pathway antibodies  Chemokine Signaling Pathway Diagram
 Chemokine Signaling Pathway antibodies  Chemokine Signaling Pathway Diagram
 Class A/1 (Rhodopsin-like Receptors) Pathway antibodies  Class A/1 (Rhodopsin-like Receptors) Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 G Alpha (i) Signalling Events Pathway antibodies  G Alpha (i) Signalling Events Pathway Diagram
 GPCR Downstream Signaling Pathway antibodies  GPCR Downstream Signaling Pathway Diagram
 GPCR Ligand Binding Pathway antibodies  GPCR Ligand Binding Pathway Diagram
 Peptide Ligand-binding Receptors Pathway antibodies  Peptide Ligand-binding Receptors Pathway Diagram
 Disease Name  Pubmed Publications
 Inflammation Antibodies  >246 publications with CCL20 and Inflammation
 Necrosis Antibodies  >164 publications with CCL20 and Necrosis
 Neoplasms Antibodies  >115 publications with CCL20 and Neoplasms
 Skin Diseases Antibodies  >79 publications with CCL20 and Skin Diseases
 Carcinoma Antibodies  >63 publications with CCL20 and Carcinoma
 Intestinal Diseases Antibodies  >60 publications with CCL20 and Intestinal Diseases
 Psoriasis Antibodies  >39 publications with CCL20 and Psoriasis
 Arthritis, Rheumatoid Antibodies  >39 publications with CCL20 and Arthritis, Rheumatoid
 Nervous System Diseases Antibodies  >36 publications with CCL20 and Nervous System Diseases
 Neoplasms, Experimental Antibodies  >27 publications with CCL20 and Neoplasms, Experimental
Organs/Tissues associated with CCL20 elisa kit
 Organ/Tissue Name  Pubmed Publications
 Intestine Antibodies  >89 publications with CCL20 and Intestine
 Mammary Gland Antibodies  >2 publications with CCL20 and Mammary Gland
 Lymphoreticular Antibodies  >1 publications with CCL20 and Lymphoreticular
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