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DPP4 elisa kit :: Bovine dipeptidyl-peptidase 4 ELISA Kit

Scan QR to view Datasheet Catalog #    MBS932554
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells (Limit 1)  /  $250 +1 FREE 8GB USB
48-Strip-Wells  /  $550 +1 FREE 8GB USB
96-Strip-Wells  /  $795 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,890 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,465 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

dipeptidyl-peptidase 4 (DPP4), ELISA Kit

 Also Known As   

Bovine Dipeptidyl peptidase 4, DPP4 ELISA Kit

 Product Synonym Names    Bovine Dipeptidyl peptidase 4 (DPP4) ELISA kit; ADABP; ADCP2; CD26; DPPIV; TP103; T-cell activation antigen CD26; adenosine deaminase complexing protein 2; dipeptidylpeptidase 4; dipeptidylpeptidase IV; dipeptidylpeptidase IV (CD26; adenosine deamina; dipeptidyl-peptidase 4
 Product Gene Name   

DPP4 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS932554 Testing Data    Testing Data PDF
 3D Structure    ModBase 3D Structure for P81425
 Species Reactivity    Bovine
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 Specificity    This assay has high sensitivity and excellent specificity for detection of bovine DPP4. No significant cross-reactivity or interference between bovine DPP4 and analogues was observed.
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 Samples    Serum, plasma, tissue homogenates
 Assay Type    Competitive
 Detection Range    0.312 ng/ml -20 ng/ml.
 Sensitivity    The minimum detectable dose of bovine DPP4 is typically less than 0.156 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest bovine DPP4 concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
 Inter-assay Precision    Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
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 Preparation and Storage    Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of DPP4 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for DPP4 purchase    MBS932554 is a ready-to-use microwell, strip plate Competitive ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the dipeptidyl-peptidase 4 (DPP4) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 0.312 ng/ml -20 ng/ml in biological research samples containing DPP4, with an estimated sensitivity of The minimum detectable dose of bovine DPP4 is typically less than 0.156 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest bovine DPP4 concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations. The ELISA analytical biochemical technique of the MBS932554 kit is based on DPP4 antibody-DPP4 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect DPP4 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, DPP4. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, tissue homogenates. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for DPP4 elisa kit

   Principle of the assay: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with DPP4. Standards or samples are added to the appropriate microtiter plate wells with Horseradish Peroxidase (HRP) conjugated antibody preparation specific for DPP4. The competitive inhibition reaction is launched between with pre-coated DPP4 and DPP4 in samples. A substrate solution is added to the wells and the color develops in opposite to the amount of DPP4 in the sample. The color development is stopped and the intensity of the color is measured.
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 Typical Testing Data/Standard Curve (for reference only) of DPP4 elisa kit    DPP4 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS932554. Click to request current manual
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NCBI/Uniprot data below describe general gene information for DPP4. It may not necessarily be applicable to this product.
 NCBI GI #    27806655
 NCBI GeneID    281122
 NCBI Accession #    NP_776464.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_174039.2 [Other Products]
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 UniProt Primary Accession #    P81425 [Other Products]
 UniProt Secondary Accession #    Q3ZCC2; Q8WMG8 [Other Products]
 UniProt Related Accession #    P81425 [Other Products]
 Molecular Weight    88,369 Da
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 NCBI Official Full Name    dipeptidyl peptidase 4
 NCBI Official Symbol    DPP4 [Similar Products]
 NCBI Official Synonym Symbols   
CD26; DPPIV
[Similar Products]
 NCBI Protein Information    dipeptidyl peptidase 4; ACT3; WC10; ADCP-I; DPP IV; activation molecule 3; dipeptidyl peptidase IV; T-cell activation antigen CD26; adenosine deaminase complexing protein; dipeptidyl-peptidase 4 (CD26, adenosine deaminase complexing protein 2); dipeptidylpeptidase IV (CD26, adenosine deaminase complexing protein 2)
 UniProt Protein Name    Dipeptidyl peptidase 4
 UniProt Synonym Protein Names   
Activation molecule 3; ACT3; Adenosine deaminase complexing protein; ADCP-I; Dipeptidyl peptidase IV; DPP IV; T-cell activation antigen CD26; WC10; CD_antigen: CD26Cleaved into the following 2 chains:Dipeptidyl peptidase 4 membrane formAlternative name(s):Dipeptidyl peptidase IV membrane form
 Protein Family    Dipeptidyl peptidase
 UniProt Gene Name    DPP4 [Similar Products]
 UniProt Synonym Gene Names    CD26; ACT3; ADCP-I; DPP IV [Similar Products]
 UniProt Entry Name    DPP4_BOVIN
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 UniProt Comments for DPP4    Function: Cell surface glycoprotein receptor involved in the costimulatory signal essential for T-cell receptor (TCR)-mediated T-cell activation. Acts as a positive regulator of T-cell coactivation, by binding at least ADA, CAV1, IGF2R, and PTPRC. Its binding to CAV1 and CARD11 induces T-cell proliferation and NF-kappa-B activation in a T-cell receptor/CD3-dependent manner. Its interaction with ADA also regulates lymphocyte-epithelial cell adhesion. In association with FAP is involved in the pericellular proteolysis of the extracellular matrix (ECM), the migration and invasion of endothelial cells into the ECM. May be involved in the promotion of lymphatic endothelial cells adhesion, migration and tube formation. When overexpressed, enhanced cell proliferation, a process inhibited by GPC3. Acts also as a serine exopeptidase with a dipeptidyl peptidase activity that regulates various physiological processes by cleaving peptides in the circulation, including many chemokines, mitogenic growth factors, neuropeptides and peptide hormones. Removes N-terminal dipeptides sequentially from polypeptides having unsubstituted N-termini provided that the penultimate residue is proline

Catalytic activity: Release of an N-terminal dipeptide, Xaa-Yaa-|-Zaa-, from a polypeptide, preferentially when Yaa is Pro, provided Zaa is neither Pro nor hydroxyproline.

Enzyme regulation: Inhibited by GPC3 and diprotin A
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 Research Articles on DPP4    1. Results describe a proline-rich cytokine from neurosecretory granules that represents a new natural substrate for Dipeptidyl Peptidase IV (DPPIV).
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Pathways associated with DPP4 elisa kitDiseases associated with DPP4 elisa kit
 Products by Pathway  Pathway Diagram
 Incretin Synthesis, Secretion, And Inactivation Pathway antibodies  Incretin Synthesis, Secretion, And Inactivation Pathway Diagram
 Metabolism Of Proteins Pathway antibodies  Metabolism Of Proteins Pathway Diagram
 Peptide Hormone Metabolism Pathway antibodies  Peptide Hormone Metabolism Pathway Diagram
 Protein Digestion And Absorption Pathway antibodies  Protein Digestion And Absorption Pathway Diagram
 Protein Digestion And Absorption Pathway antibodies  Protein Digestion And Absorption Pathway Diagram
 Synthesis, Secretion, And Inactivation Of Glucagon-like Peptide-1 (GLP-1) Pathway antibodies  Synthesis, Secretion, And Inactivation Of Glucagon-like Peptide-1 (GLP-1) Pathway Diagram
 Synthesis, Secretion, And Inactivation Of Glucose-dependent Insulinotropic Polypeptide (GIP) Pathway antibodies  Synthesis, Secretion, And Inactivation Of Glucose-dependent Insulinotropic Polypeptide (GIP) Pathway Diagram
 Disease Name  Pubmed Publications
 Cardiovascular Diseases Antibodies  >63 publications with DPP4 and Cardiovascular Diseases
 Inflammation Antibodies  >49 publications with DPP4 and Inflammation
 Disease Models, Animal Antibodies  >49 publications with DPP4 and Disease Models, Animal
 Weight Loss Antibodies  >35 publications with DPP4 and Weight Loss
 Heart Diseases Antibodies  >24 publications with DPP4 and Heart Diseases
 Liver Diseases Antibodies  >24 publications with DPP4 and Liver Diseases
 Kidney Diseases Antibodies  >19 publications with DPP4 and Kidney Diseases
 Adenocarcinoma Antibodies  >17 publications with DPP4 and Adenocarcinoma
 Necrosis Antibodies  >16 publications with DPP4 and Necrosis
 Hypertension Antibodies  >16 publications with DPP4 and Hypertension
Organs/Tissues associated with DPP4 elisa kit
 Organ/Tissue Name  Pubmed Publications
 Pancreas Antibodies  >57 publications with DPP4 and Pancreas
 Liver Antibodies  >30 publications with DPP4 and Liver
 Kidney Antibodies  >23 publications with DPP4 and Kidney
 Intestine Antibodies  >19 publications with DPP4 and Intestine
 Skin Antibodies  >14 publications with DPP4 and Skin
 Brain Antibodies  >11 publications with DPP4 and Brain
 Adipose Tissue Antibodies  >8 publications with DPP4 and Adipose Tissue
 Ovary Antibodies  >2 publications with DPP4 and Ovary
 Abomasum Antibodies  >1 publications with DPP4 and Abomasum
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