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FGF21 elisa kit :: Bovine fibroblast growth factor 21 ELISA Kit

Scan QR to view Datasheet Catalog #    MBS944301
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells (LIMIT 1)  /  $250 +1 FREE 8GB USB
48-Strip-Wells  /  $550 +1 FREE 8GB USB
96-Strip-Wells  /  $795 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,890 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,465 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

fibroblast growth factor 21 (FGF21), ELISA Kit

★Popular Item★
 Also Known As   

Bovine fibroblast growth factor 21, FGF21 ELISA Kit

 Product Synonym Names    Bovine fibroblast growth factor 21 (FGF21) ELISA kit; fibroblast growth factor 21
 Product Gene Name   

FGF21 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS944301 Testing Data    Testing Data PDF
 OMIM    609436
 3D Structure    ModBase 3D Structure for Q9NSA1
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 Species Reactivity    Bovine
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 Specificity    This assay has high sensitivity and excellent specificity for detection of Bovine FGF21. No significant cross-reactivity or interference between Bovine FGF21 and analogues was observed.
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 Samples    Serum, plasma, tissue homogenates
 Assay Type    Sandwich
 Detection Range    62.5 pg/ml-4000 pg/ml
 Sensitivity    15.6 pg/ml
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): CV% is less than 8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV% is less than 10%
Three samples of known concentration were tested in twenty assays to assess.
 Detection Wavelength    450nm
 Sample Volume    50-100ul
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 Preparation and Storage    Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of FGF21 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for FGF21 purchase    MBS944301 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the fibroblast growth factor 21 (FGF21) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 62.5 pg/ml-4000 pg/ml in biological research samples containing FGF21, with an estimated sensitivity of 15.6 pg/ml. The ELISA analytical biochemical technique of the MBS944301 kit is based on FGF21 antibody-FGF21 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect FGF21 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, FGF21. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, tissue homogenates. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for FGF21 elisa kit

   Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for FGF21 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any FGF21 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for FGF21 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of FGF21 bound in the initial step. The color development is stopped and the intensity of the color is measured.
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 Typical Testing Data/Standard Curve (for reference only) of FGF21 elisa kit    FGF21 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS944301. Click to request current manual
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NCBI/Uniprot data below describe general gene information for FGF21. It may not necessarily be applicable to this product.
 NCBI GI #    9506597
 NCBI GeneID    26291
 NCBI Accession #    NP_061986.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_019113.2 [Other Products]
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 UniProt Primary Accession #    Q9NSA1 [Other Products]
 UniProt Secondary Accession #    Q8N683 [Other Products]
 UniProt Related Accession #    Q9NSA1 [Other Products]
 Molecular Weight    22,300 Da
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 NCBI Official Full Name    fibroblast growth factor 21
 NCBI Official Synonym Full Names    fibroblast growth factor 21
 NCBI Official Symbol    FGF21 [Similar Products]
 NCBI Protein Information    fibroblast growth factor 21
 UniProt Protein Name    Fibroblast growth factor 21
 Protein Family    Fibroblast growth factor
 UniProt Gene Name    FGF21 [Similar Products]
 UniProt Synonym Gene Names    UNQ3115/PRO10196; FGF-21 [Similar Products]
 UniProt Entry Name    FGF21_HUMAN
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 NCBI Summary for FGF21    The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities and are involved in a variety of biological processes including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. The function of this growth factor has not yet been determined. [provided by RefSeq, Jul 2008]
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 UniProt Comments for FGF21    FGF21: Stimulates glucose uptake in differentiated adipocytes via the induction of glucose transporter SLC2A1/GLUT1 expression (but not SLC2A4/GLUT4 expression). Activity requires the presence of KLB. Belongs to the heparin-binding growth factors family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 19q13.33

Cellular Component: extracellular region

Molecular Function: protein binding; growth factor activity; fibroblast growth factor receptor binding

Biological Process: positive regulation of glucose import; cell-cell signaling; positive regulation of cell proliferation; positive regulation of low-density lipoprotein receptor biosynthetic process; signal transduction
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 Research Articles on FGF21    1. Repeated measurements following 25 subjects for 2 years revealed no significant correlation between FGF21 concentration and Mitochondrial Disease progression
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with FGF21 elisa kitPathways associated with FGF21 elisa kit
 Reference Product  PubMed Publications
 KLB elisa kit  >13 publications with FGF21 and KLB
 Products by Pathway  Pathway Diagram
 MAPK Signaling Pathway antibodies  MAPK Signaling Pathway Diagram
 MAPK Signaling Pathway antibodies  MAPK Signaling Pathway Diagram
 Melanoma Pathway antibodies  Melanoma Pathway Diagram
 Melanoma Pathway antibodies  Melanoma Pathway Diagram
 Pathways In Cancer antibodies  Pathways In Cancer Diagram
 Regulation Of Actin Cytoskeleton Pathway antibodies  Regulation Of Actin Cytoskeleton Pathway Diagram
 Regulation Of Actin Cytoskeleton Pathway antibodies  Regulation Of Actin Cytoskeleton Pathway Diagram
 Regulation Of Actin Cytoskeleton Pathway antibodies  Regulation Of Actin Cytoskeleton Pathway Diagram
Diseases associated with FGF21 elisa kit
 Disease Name  Pubmed Publications
 Obesity Antibodies  >69 publications with FGF21 and Obesity
 Insulin Resistance Antibodies  >60 publications with FGF21 and Insulin Resistance
 Fatty Liver Antibodies  >59 publications with FGF21 and Fatty Liver
 Diabetes Mellitus Antibodies  >50 publications with FGF21 and Diabetes Mellitus
 Hyperinsulinism Antibodies  >42 publications with FGF21 and Hyperinsulinism
 Liver Diseases Antibodies  >36 publications with FGF21 and Liver Diseases
 Diabetes Mellitus, Type 2 Antibodies  >35 publications with FGF21 and Diabetes Mellitus, Type 2
 Weight Loss Antibodies  >18 publications with FGF21 and Weight Loss
 Hyperglycemia Antibodies  >16 publications with FGF21 and Hyperglycemia
 Inflammation Antibodies  >14 publications with FGF21 and Inflammation
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