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IFNG elisa kit :: Bovine interferon, gamma ELISA Kit

Scan QR to view Datasheet Catalog #    MBS704330
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells (Limit 1)  /  $250 +1 FREE 8GB USB
48-Strip-Wells  /  $450 +1 FREE 8GB USB
96-Strip-Wells  /  $640 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,615 +3 FREE 8GB USB
10x96-Strip-Wells  /  $4,940 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

interferon, gamma (IFNG), ELISA Kit

 Also Known As   

Bovine Interferon gamma, IFN-gamma ELISA Kit

 Product Synonym Names    Bovine Interferon gamma; IFN-gamma ELISA Kit; IFG; IFI; ; interferon; gamma
 Product Gene Name   

IFNG elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Manual Insert    Download PDF Manual View PDF Manual
 MBS704330 Testing Data    Testing Data PDF
 3D Structure    ModBase 3D Structure for P07353
 Species Reactivity    Bovine
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 Specificity    This assay has high sensitivity and excellent specificity for detection of bovine IFN -gamma. No significant cross-reactivity or interference between bovine IFN -gamma and analogues was observed.
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 Samples    Serum, plasma, tissue homogenates
 Assay Type    Sandwich
 Detection Range    31.25 pg/ml -2000 pg/ml.
 Sensitivity    The minimum detectable dose of bovine IFN -gamma is typically less than 7.81 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
 Inter-assay Precision    Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
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 Preparation and Storage    Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay.
 Other Notes    Small volumes of IFNG elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for IFNG purchase    MBS704330 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the interferon, gamma (IFNG) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 31.25 pg/ml -2000 pg/ml in biological research samples containing IFNG, with an estimated sensitivity of The minimum detectable dose of bovine IFN -gamma is typically less than 7.81 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations. The ELISA analytical biochemical technique of the MBS704330 kit is based on IFNG antibody-IFNG antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect IFNG antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, IFNG. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, tissue homogenates. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Product Description specifically for IFNG elisa kit

   Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for IFN-gamma has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IFN-gamma present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for IFN-gamma is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IFN-gamma bound in the initial step. The color development is stopped and the intensity of the color is measured.
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 Typical Testing Data/Standard Curve (for reference only) of IFNG elisa kit    IFNG elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS704330
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NCBI/Uniprot data below describe general gene information for IFNG. It may not necessarily be applicable to this product.
 NCBI GI #    31982949
 NCBI GeneID    281237
 NCBI Accession #    NP_776511.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_174086.1 [Other Products]
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 UniProt Primary Accession #    P07353 [Other Products]
 UniProt Secondary Accession #    A7L687; A9QWQ4 [Other Products]
 UniProt Related Accession #    P07353 [Other Products]
 Molecular Weight    19,393 Da
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 NCBI Official Full Name    interferon gamma
 NCBI Official Synonym Full Names    interferon, gamma
 NCBI Official Symbol    IFNG [Similar Products]
 NCBI Protein Information    interferon gamma; boIFNG; IFN-gamma; interferon-gamma; interferon gamma type 2
 UniProt Protein Name    Interferon gamma
 Protein Family    Interferon
 UniProt Gene Name    IFNG [Similar Products]
 UniProt Synonym Gene Names    BoIFNG; IFN-gamma [Similar Products]
 UniProt Entry Name    IFNG_BOVIN
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 UniProt Comments for IFNG    Function: Produced by lymphocytes activated by specific antigens or mitogens. IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions. It is a potent activator of macrophages, it has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons.
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 Research Articles on IFNG    1. These results support pregnancy as type 2 immune response biased, with increases of IFN-gamma occurring after parturition and an increase in IL-4 production before calving.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with IFNG elisa kitPathways associated with IFNG elisa kit
 Reference Product  PubMed Publications
 TNF elisa kit  >92 publications with IFNG and TNF
 IFNGR1 elisa kit  >21 publications with IFNG and IFNGR1
 IFNGR2 elisa kit  >11 publications with IFNG and IFNGR2
 Products by Pathway  Pathway Diagram
 African Trypanosomiasis Pathway antibodies  African Trypanosomiasis Pathway Diagram
 African Trypanosomiasis Pathway antibodies  African Trypanosomiasis Pathway Diagram
 Allograft Rejection Pathway antibodies  Allograft Rejection Pathway Diagram
 Allograft Rejection Pathway antibodies  Allograft Rejection Pathway Diagram
 Amoebiasis Pathway antibodies  Amoebiasis Pathway Diagram
 Amoebiasis Pathway antibodies  Amoebiasis Pathway Diagram
 Antigen Processing And Presentation Pathway antibodies  Antigen Processing And Presentation Pathway Diagram
 Antigen Processing And Presentation Pathway antibodies  Antigen Processing And Presentation Pathway Diagram
 Chagas Disease (American Trypanosomiasis) Pathway antibodies  Chagas Disease (American Trypanosomiasis) Pathway Diagram
 Chagas Disease (American Trypanosomiasis) Pathway antibodies  Chagas Disease (American Trypanosomiasis) Pathway Diagram
Diseases associated with IFNG elisa kitOrgans/Tissues associated with IFNG elisa kit
 Disease Name  Pubmed Publications
 Necrosis Antibodies  >135 publications with IFNG and Necrosis
 Autoimmune Diseases Antibodies  >84 publications with IFNG and Autoimmune Diseases
 Respiratory Tract Diseases Antibodies  >63 publications with IFNG and Respiratory Tract Diseases
 Hepatitis Antibodies  >31 publications with IFNG and Hepatitis
 Lymphoproliferative Disorders Antibodies  >20 publications with IFNG and Lymphoproliferative Disorders
 Diabetes Mellitus, Type 1 Antibodies  >17 publications with IFNG and Diabetes Mellitus, Type 1
 HIV Infections Antibodies  >16 publications with IFNG and HIV Infections
 Arthritis, Rheumatoid Antibodies  >16 publications with IFNG and Arthritis, Rheumatoid
 Hepatitis C Antibodies  >13 publications with IFNG and Hepatitis C
 Leishmaniasis Antibodies  >9 publications with IFNG and Leishmaniasis
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >259 publications with IFNG and Blood
 Kidney Antibodies  >23 publications with IFNG and Kidney
 Lymph Node Antibodies  >16 publications with IFNG and Lymph Node
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