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IL1B elisa kit :: Bovine interleukin 1, beta ELISA Kit

Scan QR to view Datasheet Catalog #    MBS703996
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells (LIMIT 1)  /  $250 +1 FREE 8GB USB
48-Strip-Wells  /  $450 +1 FREE 8GB USB
96-Strip-Wells  /  $640 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,615 +3 FREE 8GB USB
10x96-Strip-Wells  /  $4,940 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

interleukin 1, beta (IL1B), ELISA Kit

★Popular Item★
 Also Known As   

Bovine Interleukin 1beta, IL- 1beta ELISA Kit

 Product Synonym Names    Bovine Interleukin 1beta (IL- 1beta) ELISA kit; IL-1; IL1-BETA; IL1F2; catabolin; preinterleukin 1 beta; pro-interleukin-1-beta; interleukin 1; beta
 Product Gene Name   

IL1B elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS703996 Testing Data    Testing Data PDF
 3D Structure    ModBase 3D Structure for P09428
 Species Reactivity    Bovine
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 Specificity    This assay has high sensitivity and excellent specificity for detection of bovine IL-1?. No significant cross-reactivity or interference between bovine IL-1? and analogues was observed.
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 Samples    Serum, plasma, tissue homogenates
 Detection Range    62.5 pg/ml -4000 pg/mL
 Sensitivity    <15.6 pg/mL
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
 Inter-assay Precision    Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
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 Preparation and Storage    Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of IL1B elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for IL1B purchase    MBS703996 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the interleukin 1, beta (IL1B) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 62.5 pg/ml -4000 pg/mL in biological research samples containing IL1B, with an estimated sensitivity of <15.6 pg/mL. The ELISA analytical biochemical technique of the MBS703996 kit is based on IL1B antibody-IL1B antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect IL1B antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, IL1B. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, tissue homogenates. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for IL1B elisa kit

   Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for IL- 1? has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IL- 1? present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for IL- 1? is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IL- 1? bound in the initial step. The color development is stopped and the intensity of the color is measured.
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 Typical Testing Data/Standard Curve (for reference only) of IL1B elisa kit    IL1B elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS703996. Click to request current manual
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NCBI/Uniprot data below describe general gene information for IL1B. It may not necessarily be applicable to this product.
 NCBI GI #    27806571
 NCBI GeneID    281251
 NCBI Accession #    NP_776518.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_174093.1 [Other Products]
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 UniProt Primary Accession #    P09428 [Other Products]
 UniProt Secondary Accession #    A9QWQ5 [Other Products]
 UniProt Related Accession #    P09428 [Other Products]
 Molecular Weight    30,760 Da
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 NCBI Official Full Name    interleukin-1 beta
 NCBI Official Synonym Full Names    interleukin 1, beta
 NCBI Official Symbol    IL1B [Similar Products]
 NCBI Protein Information    interleukin-1 beta; IL-1 beta
 UniProt Protein Name    Interleukin-1 beta
 Protein Family    Interleukin
 UniProt Gene Name    IL1B [Similar Products]
 UniProt Synonym Gene Names    IL-1 beta [Similar Products]
 UniProt Entry Name    IL1B_BOVIN
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 UniProt Comments for IL1B    Function: Produced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells.
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 Research Articles on IL1B    1. For the inflammasome-dependent IL-1beta release, bovine monocytes require ATP in addition to a primary stimulus. This IL-1beta release depends on potassium efflux, but, in contrast to human and murine monocytes, does not require calcium influx or generation of reaction oxygen and is independent of the P2X7 receptor.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with IL1B elisa kitPathways associated with IL1B elisa kit
 Reference Product  PubMed Publications
 IL1A elisa kit  >183 publications with IL1B and IL1A
 PTGS2 elisa kit  >50 publications with IL1B and PTGS2
 IL1R1 elisa kit  >36 publications with IL1B and IL1R1
 NOS2 elisa kit  >25 publications with IL1B and NOS2
 IL18 elisa kit  >24 publications with IL1B and IL18
 NFKB1 elisa kit  >20 publications with IL1B and NFKB1
 MYD88 elisa kit  >19 publications with IL1B and MYD88
 IL1R2 elisa kit  >12 publications with IL1B and IL1R2
 TRAF6 elisa kit  >10 publications with IL1B and TRAF6
 IL1RAP elisa kit  >3 publications with IL1B and IL1RAP
 Products by Pathway  Pathway Diagram
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 Apoptosis Pathway antibodies  Apoptosis Pathway Diagram
 Apoptosis Pathway antibodies  Apoptosis Pathway Diagram
 Chagas Disease (American Trypanosomiasis) Pathway antibodies  Chagas Disease (American Trypanosomiasis) Pathway Diagram
 Chagas Disease (American Trypanosomiasis) Pathway antibodies  Chagas Disease (American Trypanosomiasis) Pathway Diagram
Diseases associated with IL1B elisa kitOrgans/Tissues associated with IL1B elisa kit
 Disease Name  Pubmed Publications
 Inflammation Antibodies  >345 publications with IL1B and Inflammation
 Necrosis Antibodies  >215 publications with IL1B and Necrosis
 Skin Diseases Antibodies  >46 publications with IL1B and Skin Diseases
 Stomach Neoplasms Antibodies  >41 publications with IL1B and Stomach Neoplasms
 Arthritis, Rheumatoid Antibodies  >26 publications with IL1B and Arthritis, Rheumatoid
 Adenocarcinoma Antibodies  >23 publications with IL1B and Adenocarcinoma
 Hypersensitivity Antibodies  >19 publications with IL1B and Hypersensitivity
 Myocardial Ischemia Antibodies  >19 publications with IL1B and Myocardial Ischemia
 Asthma Antibodies  >19 publications with IL1B and Asthma
 Colitis Antibodies  >16 publications with IL1B and Colitis
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >162 publications with IL1B and Blood
 Lung Antibodies  >36 publications with IL1B and Lung
 Stomach Antibodies  >33 publications with IL1B and Stomach
 Uterus Antibodies  >28 publications with IL1B and Uterus
 Vascular Antibodies  >23 publications with IL1B and Vascular
 Brain Antibodies  >18 publications with IL1B and Brain
 Liver Antibodies  >18 publications with IL1B and Liver
 Skin Antibodies  >15 publications with IL1B and Skin
 Mouth Antibodies  >13 publications with IL1B and Mouth
 Bone Marrow Antibodies  >10 publications with IL1B and Bone Marrow
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