Antibody Protein ELISA Kit from MyBioSource header Telephone 1.858.633.0165          
1.855.MyBioSource
Fax 1.858.633.0166    Email sales@mybiosource.com 
tel/fax
menu1 Home Products Ordering Company Help menu7
bottom
   MyBioSource rightarrow ELISA Kit rightarrow LEP rightarrow Bovine LEP  rightarrow LOG IN  rightarrow MY ACCOUNT  rightarrow CART CONTENTS  rightarrow CHECKOUT 
horiz bar
MyBioSource Menu separator
separator
ELISA Kit ELISA Kit
CLIA Kit CLIA Kit
PCR Kit PCR Kit
Monoclonal Antibody Monoclonal Antibody
Polyclonal Antibody Polyclonal Antibody
Secondary Antibody Secondary Antibody
Antigen Antigen
Biochemical Biochemical
cDNA Clone cDNA Clone
siRNA siRNA
Peptide Peptide
Recombinant/Purified Protein Rec./Purified Protein

Custom ELISA Kit Custom ELISA Kit
Custom Protein Custom Protein
Custom Antibody Custom Antibody
Antibody Matched Pairs Antibody Matched Pairs
Antibody & Corresponding Blocking Peptide Pairs Antibody Peptide Pairs
Phospho-Specific Antibodies Phospho Antibodies
Products by Disease Products by Disease
Products by Pathway Products by Pathway
Products by Tissue Products by Tissue

arrow Advanced Search
arrow Submit Technical Q&A
arrow International Distributors
arrow Contact Us
separator
Our Best Sellers moreseparator
separator
 • KA2 Antibody
 • ATG16 Antibody
 • BIRC7 - Livin Antibody
 • sphingosine-1-phosphate receptor 3 (S1PR3) ELISA Kit
 • UBE3A ELISA Kit
 • Tenascin X (TNX) ELISA Kit
 • Rituximab Antibody
 • P3H2 Antibody
 • F-actin ELISA Kit
 • CD163 Antibody
 • MYL12B Recombinant Protein
 • Human IgG Fac fragment Antibody
 • Cystatin-D (CST5) ELISA Kit
 • CD13 - Aminopeptidase-N (ANPEP) Antibody
 downarrow more ...
separator
separator
DatasheetFull DatasheetPrinter Friendly DatasheetPrint This DatasheetAdd to Compare ListHave Questions? Ask UsRequest for a Quotation today

LEP elisa kit :: Bovine leptin ELISA Kit

Scan QR to view Datasheet Catalog #    MBS703026
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells (LIMIT 1)  /  $250 +1 FREE 8GB USB
48-Strip-Wells  /  $320 +1 FREE 8GB USB
96-Strip-Wells  /  $445 +1 FREE 8GB USB
5x96-Strip-Wells  /  $1,880 +2 FREE 8GB USB
10x96-Strip-Wells  /  $3,540 +4 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

leptin, ELISA Kit

★Popular Item★
 Also Known As   

Bovine Leptin, LEP ELISA Kit

 Product Synonym Names    Bovine Leptin; LEP ELISA Kit; FLJ94114; OB; OBS; leptin (murine obesity homolog) ; leptin (obesity homolog; mouse) ; obese; mouse; homolog of; obesity factor; leptin
 Product Gene Name   

LEP elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
Table BarTOPTable Bar
  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS703026 COA    COA PDF
 MBS703026 Testing Data    Testing Data PDF
 3D Structure    ModBase 3D Structure for P50595
 Species Reactivity    Bovine
Section Bar
 Specificity    This assay has high sensitivity and excellent specificity for detection of bovine LEP. No significant cross-reactivity or interference between bovine LEP and analogues was observed.
Section Bar
 Samples    Serum, plasma, tissue homogenates
 Assay Type    Quantitative Competitive
 Detection Range    3.12 ng/ml-50 ng/ml
 Sensitivity    Typically less than 3.12 ng/ml.
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): CV%<15%. Three samples of known concentration were tested twenty times on one plate to assess
 Inter-assay Precision    Inter-assay Precision (Precision between assays): CV%<15%. Three samples of known concentration were tested in twenty assays to assess.
Section Bar
 Preparation and Storage    Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of LEP elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for LEP purchase    MBS703026 is a ready-to-use microwell, strip plate Quantitative Competitive ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the leptin, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 3.12 ng/ml-50 ng/ml in biological research samples containing LEP, with an estimated sensitivity of Typically less than 3.12 ng/ml. The ELISA analytical biochemical technique of the MBS703026 kit is based on LEP antibody-LEP antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect LEP antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, LEP. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, tissue homogenates. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Table BarTOPTable Bar
 

Related Product Information for LEP elisa kit

   Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to LEP. Standards or samples are added to the appropriate microtiter plate wells with biotin-conjugated LEP. A competitive inhibition reaction is launched between LEP (Standards or samples) and biotin-conjugated LEP with the pre-coated antibody specific for LEP. The more amount of LEP in samples, the less antibody bound by biotin-conjugated LEP. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Substrate solution is added to the wells and the color develops in opposite to the amount of LEP in the sample. The color development is stopped and the intensity of the color is measured.
Section Bar
 Typical Testing Data/Standard Curve (for reference only) of LEP elisa kit    LEP elisa kit Typical Testing Data/Standard Curve (for reference only) image
Table BarTOPTable Bar
Sample Manual Insert of MBS703026. Click to request current manual
Table BarTOPTable Bar
NCBI/Uniprot data below describe general gene information for LEP. It may not necessarily be applicable to this product.
 NCBI GI #    87196505
 NCBI GeneID    280836
 NCBI Accession #    NP_776353.2 [Other Products]
 NCBI GenBank Nucleotide #    NM_173928.2 [Other Products]
Section Bar
 UniProt Primary Accession #    P50595 [Other Products]
 UniProt Secondary Accession #    O97918; Q5EAE4; Q95133; Q9TSZ9 [Other Products]
 UniProt Related Accession #    P50595 [Other Products]
 Molecular Weight    18,716 Da
Table BarTOPTable Bar
 NCBI Official Full Name    leptin
 NCBI Official Synonym Full Names    leptin<
 NCBI Official Symbol    LEP [Similar Products]
 NCBI Official Synonym Symbols   
ob
[Similar Products]
 NCBI Protein Information    leptin; obese; obesity factor; leptin (obesity homolog, mouse)
 UniProt Protein Name    Leptin
 UniProt Synonym Protein Names   
Obesity factor
 Protein Family    Leptin
 UniProt Gene Name    LEP [Similar Products]
 UniProt Synonym Gene Names    OB; OBS [Similar Products]
 UniProt Entry Name    LEP_BOVIN
Section Bar
 UniProt Comments for LEP    Function: May function as part of a signaling pathway that acts to regulate the size of the body fat depot. An increase in the level of LEP may act directly or indirectly on the CNS to inhibit food intake and/or regulate energy expenditure as part of a homeostatic mechanism to maintain constancy of the adipose mass.
Table BarTOPTable Bar
 Research Articles on LEP    1. SNP LEP significantly affected milk, protein and fat yield (P<0.05), and age at first calving (P<0.01) in analyzed population of cows. SNP LEPR/T945M affected significantly calving interval (P<0.01) only
Table BarTOPTable Bar
 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Table BarTOPTable Bar
Pathways associated with LEP elisa kitDiseases associated with LEP elisa kit
 Products by Pathway  Pathway Diagram
 AMPK Signaling Pathway antibodies  AMPK Signaling Pathway Diagram
 AMPK Signaling Pathway antibodies  AMPK Signaling Pathway Diagram
 Adipocytokine Signaling Pathway antibodies  Adipocytokine Signaling Pathway Diagram
 Adipocytokine Signaling Pathway antibodies  Adipocytokine Signaling Pathway Diagram
 Adipogenesis Pathway antibodies  Adipogenesis Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 Developmental Biology Pathway antibodies  Developmental Biology Pathway Diagram
 Incretin Synthesis, Secretion, And Inactivation Pathway antibodies  Incretin Synthesis, Secretion, And Inactivation Pathway Diagram
 Jak-STAT Signaling Pathway antibodies  Jak-STAT Signaling Pathway Diagram
 Disease Name  Pubmed Publications
 Obesity Antibodies  >341 publications with LEP and Obesity
 Pain Antibodies  >170 publications with LEP and Pain
 Insulin Resistance Antibodies  >110 publications with LEP and Insulin Resistance
 Hyperinsulinism Antibodies  >73 publications with LEP and Hyperinsulinism
 Weight Gain Antibodies  >57 publications with LEP and Weight Gain
 Weight Loss Antibodies  >54 publications with LEP and Weight Loss
 Cardiovascular Diseases Antibodies  >51 publications with LEP and Cardiovascular Diseases
 Fatty Liver Antibodies  >43 publications with LEP and Fatty Liver
 Inflammation Antibodies  >41 publications with LEP and Inflammation
 Liver Diseases Antibodies  >37 publications with LEP and Liver Diseases
Organs/Tissues associated with LEP elisa kit
 Organ/Tissue Name  Pubmed Publications
 Bone Antibodies  >13 publications with LEP and Bone
 Connective Tissue Antibodies  >4 publications with LEP and Connective Tissue
 Heart Antibodies  >3 publications with LEP and Heart
 Muscle Antibodies  >3 publications with LEP and Muscle
 Brain Antibodies  >3 publications with LEP and Brain
 Vascular Antibodies  >2 publications with LEP and Vascular
 Embryonic Tissue Antibodies  >2 publications with LEP and Embryonic Tissue
 Ear Antibodies  >1 publications with LEP and Ear
 Kidney Antibodies  >1 publications with LEP and Kidney
 Umbilical Cord Antibodies  >1 publications with LEP and Umbilical Cord
Table BarTOPTable Bar
horiz bar
 SSL   Follow us on Facebook Follow us onTwitter Follow us on Google Plus Connect us on LinkedIn Subscribe to our RSS Feed for latest products and special promotions