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PAM elisa kit :: Bovine peptidylglycine alpha-amidating monooxygenase ELISA Kit

Scan QR to view Datasheet Catalog #    MBS9333038 PAM elisa kit
Unit / Price
48-Strip-Wells  /  $470 +1 FREE 8GB USB
96-Strip-Wells  /  $680 +1 FREE 8GB USB
5x96-Strip-Wells  /  $3,100 +3 FREE 8GB USB
10x96-Strip-Wells  /  $6,095 +7 FREE 8GB USB
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

peptidylglycine alpha-amidating monooxygenase (PAM), ELISA Kit

 Also Known As   

Bovine Peptidyl-glycine alpha-amidating monooxygenase, PAM ELISA Kit

 Product Synonym Names    Bovine Peptidyl-glycine alpha-amidating monooxygenase (PAM) ELISA kit; PAL; PHM; pancreatic peptidylglycine alpha-amidating monooxygenase; peptidyl alpha-amidating enzyme; peptidyl-alpha-hydroxyglycine alpha-amidating lyase; peptidylglycine 2-hydroxylase; peptidylglycine a; peptidylglycine alpha-amidating monooxygenase
 Product Gene Name   

PAM elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Request for Current Manual Insert    Request Current Manual
 3D Structure    ModBase 3D Structure for P10731
 Species Reactivity    Bovine
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 Preparation and Storage    Store all reagents at 2-8 degree C
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of PAM elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for PAM purchase    MBS9333038 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the peptidylglycine alpha-amidating monooxygenase (PAM) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing PAM. The ELISA analytical biochemical technique of the MBS9333038 kit is based on PAM antibody-PAM antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect PAM antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, PAM. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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NCBI/Uniprot data below describe general gene information for PAM. It may not necessarily be applicable to this product.
 NCBI GI #    27806825
 NCBI GeneID    280890
 NCBI Accession #    NP_776373.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_173948.2 [Other Products]
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 UniProt Primary Accession #    P10731 [Other Products]
 UniProt Related Accession #    P10731 [Other Products]
 Molecular Weight    108,177 Da
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 NCBI Official Full Name    peptidyl-glycine alpha-amidating monooxygenase
 NCBI Official Synonym Full Names    peptidylglycine alpha-amidating monooxygenase<
 NCBI Official Symbol    PAM [Similar Products]
 NCBI Protein Information    peptidyl-glycine alpha-amidating monooxygenase
 UniProt Protein Name    Peptidyl-glycine alpha-amidating monooxygenase
 UniProt Synonym Protein Names   
Peptidylamidoglycolate lyase; PAL
 Protein Family    Phenylalanine aminomutase
 UniProt Gene Name    PAM [Similar Products]
 UniProt Synonym Gene Names    PAM; PHM; PAL [Similar Products]
 UniProt Entry Name    AMD_BOVIN
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 UniProt Comments for PAM    Function: Bifunctional enzyme that catalyzes 2 sequential steps in C-terminal alpha-amidation of peptides. The monooxygenase part produces an unstable peptidyl(2-hydroxyglycine) intermediate that is dismutated to glyoxylate and the corresponding desglycine peptide amide by the lyase part. C-terminal amidation of peptides such as neuropeptides is essential for full biological activity.1 PublicationManual assertion based on experiment in:Ref.2

Catalytic activity: Peptidylglycine + ascorbate + O2 = peptidyl(2-hydroxyglycine) + dehydroascorbate + H2O.

Cofactor: Zinc; for the lyase reaction
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 Research Articles on PAM    1. These data suggest that cortisol affects oxytocin synthesis and secretion in bovine ovarian cells, by acting on the expression of key genes, that may impair ovary
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Diseases associated with PAM elisa kitOrgans/Tissues associated with PAM elisa kit
 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >792 publications with PAM and Neoplasms
 Nervous System Diseases Antibodies  >410 publications with PAM and Nervous System Diseases
 Drug Toxicity Antibodies  >327 publications with PAM and Drug Toxicity
 Immune System Diseases Antibodies  >292 publications with PAM and Immune System Diseases
 Skin Diseases Antibodies  >267 publications with PAM and Skin Diseases
 Cardiovascular Diseases Antibodies  >243 publications with PAM and Cardiovascular Diseases
 Brain Diseases Antibodies  >218 publications with PAM and Brain Diseases
 Lung Diseases Antibodies  >191 publications with PAM and Lung Diseases
 Inflammation Antibodies  >186 publications with PAM and Inflammation
 Necrosis Antibodies  >175 publications with PAM and Necrosis
 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >380 publications with PAM and Brain
 Lung Antibodies  >367 publications with PAM and Lung
 Skin Antibodies  >199 publications with PAM and Skin
 Muscle Antibodies  >173 publications with PAM and Muscle
 Kidney Antibodies  >155 publications with PAM and Kidney
 Liver Antibodies  >115 publications with PAM and Liver
 Intestine Antibodies  >60 publications with PAM and Intestine
 Ovary Antibodies  >35 publications with PAM and Ovary
 Lens Antibodies  >35 publications with PAM and Lens
 Uterus Antibodies  >31 publications with PAM and Uterus
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