Intended Uses
This Human CRP ELISA kit is to be used for the in vitro quantitative determination of human c reactive protein (CRP) concentrations in serum, plasma and cell culture supernatant. This kit is intended FOR LABORATORY RESEARCH USE only
Product Note
Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our
technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
Other Notes
Small volumes of CRP elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for CRP purchase
MBS590047 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the C Reactive Protein (CRP) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing CRP. The ELISA analytical biochemical technique of the MBS590047 kit is based on CRP antibody-CRP antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect CRP antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, CRP. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
CRP elisa kit
Principle of the assay: This CRP enzyme-linked immunosorbent assay (ELISA) applies a technique called a quantitative sandwich immunoassay. The microtiter plate provided in this kit has been pre-coated with a monoclonal antibody specific for CRP. Standards or samples are then added to the appropriate microtiter plate wells and incubated. CRP, if present, will bind and become immobilized by the antibody pre-coated on the wells. The microtiter plate wells are thoroughly washed to remove unbound CRP and other components of sample. In order to quantitate the amount of CRP present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated antibody specific for CRP is added to each well to "sandwich" the CRP immobilized during the second incubation. The wells are thoroughly washed to remove all unbound HRP-conjugated antibodies and a TMB (3,3'5,5' tetramethyl-benzidine) substrate solution is added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain CRP and enzyme-conjugated antibody will exhibit a change in colour. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the colour change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. In order to measure the concentration of CRP in the samples, this kit standard (ready-to use) is assayed at the same time as the samples (diluted if necessary with Sample Diluent). This allows the operator to produce a standard curve of Optical Density (O.D.) versus CRP concentration (?g/mL). The concentration of CRP in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Background: Human CRP is a kind of nonimmunoglobulin serum substance, a heat labile b-globulin. It is classified in a superfamily of proteins termed pentaxins or pentraxins: cyclic, non-glycosylated structures composed of five apparently identical globular non-covalently linked subunits aggregated symmetrically. Each subunit is 23.05 kD (206 amino acids), with a total molecular weight of 117.5 kDa, and consists of 14 anti-parallel b-strands arranged in two b-sheets. CRP is an acute phase protein, originally identified and named for its ability to precipitate the C-polysaccharide of pneumococcus in the presence of calcium. It is the prototypic acute phase reactant whose presence in plasma or serum serves as a useful laboratory indicator of systemic inflammatory disease. Normally, CRP in human biological fluids is present in trace amounts (0.07-8.00 mg/L, median 0.6 mg/L). Stimulated by certain cytokines (IL-1a, IL-1b, TNF-a and b, and indirectly by IL-6), its synthesis by hepatocytes enhanced dramatically. During the acute phase response, CRP concentration can increase up to 1000-fold within a few hours. Among acute phase proteins, CRP is a fast-reacting, sensitive and the most easily measured one. It has a rapid response time, short half-life and large incremental change and its catabolism is not affected by the type of inflammation. Following acute tissue damage or during the course of infectious and non-infectious conditions, hepatic synthesis of CRP dramatically increases. Typically, mild elevations of CRP are seen in a variety of inflammatory conditions. Serum amyloid A (SAA) is another major acute phase protein whose response is highly correlated with that of CRP. Both CRP and SAA respond sensitively to several stimuli, but they differ in certain responses.