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| Product Name
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A Disintegrin And Metalloprotease 19 (ADAM19), ELISA Kit
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| Also Known As
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Canine A Disintegrin And Metalloprotease 19 ELISA Kit
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| Product Gene Name
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[Similar Products]
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| Research Use Only
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For Research Use Only. Not for use in diagnostic procedures.
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| Sample Manual Insert
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| Request for Current Manual Insert
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| OMIM
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603640
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| Species Reactivity
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Canine
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| Specificity
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This assay has high sensitivity and excellent specificity for detection of ADAM19. No significant cross-reactivity or interference between ADAM19 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between ADAM19 and all the analogues, therefore, cross reaction may still exist in some cases.
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| Samples
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Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
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| Assay Type
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Competitive
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| Detection Range
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0.5-10ng/mL
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| Sensitivity
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0.1ng/mL
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| Intended Uses
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This ADAM19 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Canine ADAM19. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
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| Preparation and Storage
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Store all reagents at 2-8 degree C.
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| Sample Preparation
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We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
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| Product Note
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Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
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| Other Notes
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Small volumes of ADAM19 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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| Searchable Terms for ADAM19 purchase
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MBS753120 is a ready-to-use microwell, strip plate Competitive ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the A Disintegrin And Metalloprotease 19 (ADAM19) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 0.5-10ng/mL in biological research samples containing ADAM19, with an estimated sensitivity of 0.1ng/mL. The ELISA analytical biochemical technique of the MBS753120 kit is based on ADAM19 antibody-ADAM19 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect ADAM19 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, ADAM19. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Principle of the assay: ADAM19 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-ADAM19 antibody and an ADAM19-HRP conjugate. The assay sample and buffer are incubated together with ADAM19-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ADAM19 concentration since ADAM19 from samples and ADAM19-HRP conjugate compete for the anti-ADAM19 antibody binding site. Since the number of sites is limited, as more sites are occupied by ADAM19 from the sample, fewer sites are left to bind ADAM19-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ADAM19 concentration in each sample is interpolated from this standard curve.
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| Product Categories/Family for ADAM19 elisa kit
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Cell Biology
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| Typical Testing Data/Standard Curve (for reference only) of ADAM19 elisa kit
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| Sample Manual Insert of MBS753120. Click to request current manual
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| NCBI/Uniprot data below describe general gene information for ADAM19. It may not necessarily be applicable to this product. |
| NCBI GI #
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38197238
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| NCBI GeneID
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8728
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| NCBI Accession #
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AAH24214.2
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[Other Products]
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| UniProt Secondary Accession #
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Q9BZL5; Q9UHP2
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[Other Products]
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| UniProt Related Accession #
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Q9H013
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[Other Products]
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| Molecular Weight
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100,848 Da
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| NCBI Official Full Name
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ADAM19 protein, partial
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| NCBI Official Synonym Full Names
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ADAM metallopeptidase domain 19
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| NCBI Official Symbol
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ADAM19
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[Similar Products]
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| NCBI Official Synonym Symbols
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MLTNB; FKSG34; MADDAM
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[Similar Products]
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| NCBI Protein Information
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disintegrin and metalloproteinase domain-containing protein 19; ADAM 19; meltrin-beta; metalloprotease-disintegrin meltrin beta; metalloprotease and disintegrin dendritic antigen marker; a disintegrin and metalloproteinase domain 19 (meltrin beta)
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| UniProt Protein Name
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Disintegrin and metalloproteinase domain-containing protein 19
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| UniProt Synonym Protein Names
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Meltrin-beta; Metalloprotease and disintegrin dendritic antigen marker; MADDAM
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| Protein Family
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Disintegrin and metalloproteinase domain-containing protein
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| UniProt Gene Name
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ADAM19
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[Similar Products]
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| UniProt Synonym Gene Names
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MLTNB; FKSG34; ADAM 19; MADDAM
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[Similar Products]
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| UniProt Entry Name
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ADA19_HUMAN
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| NCBI Summary for ADAM19
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This gene encodes a member of the ADAM (a disintegrin and metalloprotease domain) family. Members of this family are membrane-anchored proteins structurally related to snake venom disintegrins and have been implicated in a variety of biological processes involving cell-cell and cell-matrix interactions, including fertilization, muscle development, and neurogenesis. This member is a type I transmembrane protein and serves as a marker for dendritic cell differentiation. It has been demonstrated to be an active metalloproteinase, which may be involved in normal physiological processes such as cell migration, cell adhesion, cell-cell and cell-matrix interactions, and signal transduction. It is proposed to play a role in pathological processes, such as cancer, inflammatory diseases, renal diseases, and Alzheimer's disease. [provided by RefSeq, May 2013]
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| UniProt Comments for ADAM19
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ADAM19: Participates in the proteolytic processing of beta-type neuregulin isoforms which are involved in neurogenesis and synaptogenesis, suggesting a regulatory role in glial cell. Also cleaves alpha-2 macroglobulin. May be involved in osteoblast differentiation and/or osteoblast activity in bone. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Motility/polarity/chemotaxis; Membrane protein, integral; Protease; EC 3.4.24.-
Chromosomal Location of Human Ortholog: 5q33.3
Cellular Component: integral to membrane
Molecular Function: zinc ion binding; metalloendopeptidase activity; SH3 domain binding
Biological Process: heart development; membrane protein ectodomain proteolysis
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| Research Articles on ADAM19
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1. ADAM19 was upregulated in patients with ulcerative colitis and, to a lesser extent, in patients with Crohn's disease compared with normal controls. In contrast, ADAM9 and ADAM10 expression did not differ between patients with IBD and controls.
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| Precautions
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All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
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| Disclaimer
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While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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| Products associated with ADAM19 elisa kit | Diseases associated with ADAM19 elisa kit |
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| Organs/Tissues associated with ADAM19 elisa kit |
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ELISA Kit 
