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ADRA2A elisa kit :: Canine Alpha-2A adrenergic receptor (ADRA2A) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS7215126
Typical Standard Curve/Testing Data
Unit / Price
48-Strip-Wells  /  $440 +1 FREE 8GB USB
96-Strip-Wells  /  $640 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,895 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,415 +6 FREE 8GB USB
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 Product Name   

Alpha-2A adrenergic receptor (ADRA2A), ELISA Kit

★Popular Item★
 Also Known As   

Canine Alpha-2A adrenergic receptor (ADRA2A) ELISA Kit

 Product Gene Name   

ADRA2A elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    104210
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 Species Reactivity    Canine
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 Specificity    This assay has high sensitivity and excellent specificity for detection of ADRA2A. No significant cross-reactivity or interference between ADRA2A and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between ADRA2A and all the analogues, therefore, cross reaction may still exist in some cases.
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 Samples    Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
 Assay Type    Competitive
 Detection Range    1.0-25ng/mL
 Sensitivity    0.1ng/mL
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 Preparation and Storage    Store all reagents at 2-8 degree C.
 Sample Preparation    We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of ADRA2A elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for ADRA2A purchase    MBS7215126 is a ready-to-use microwell, strip plate Competitive ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Alpha-2A adrenergic receptor (ADRA2A) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 1.0-25ng/mL in biological research samples containing ADRA2A, with an estimated sensitivity of 0.1ng/mL. The ELISA analytical biochemical technique of the MBS7215126 kit is based on ADRA2A antibody-ADRA2A antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect ADRA2A antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, ADRA2A. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for ADRA2A elisa kit

   Intended Uses: This ADRA2A ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Canine ADRA2A. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Principle of the Assay: ADRA2A ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-ADRA2A antibody and an ADRA2A-HRP conjugate. The assay sample and buffer are incubated together with ADRA2A-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ADRA2A concentration since ADRA2A from samples and ADRA2A-HRP conjugate compete for the anti-ADRA2A antibody binding site. Since the number of sites is limited, as more sites are occupied by ADRA2A from the sample, fewer sites are left to bind ADRA2A-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ADRA2A concentration in each sample is interpolated from this standard curve.
 Product Categories/Family for ADRA2A elisa kit    Neuroscience
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 Typical Standard Curve/Testing Data of ADRA2A elisa kit    ADRA2A elisa kit Typical Standard Curve/Testing Data image
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Sample Manual Insert of MBS7215126. Click to request current manual
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NCBI/Uniprot data below describe general gene information for ADRA2A. It may not necessarily be applicable to this product.
 NCBI GI #    194353970
 NCBI GeneID    150
 NCBI Accession #    NP_000672.3 [Other Products]
 NCBI GenBank Nucleotide #    NM_000681.3 [Other Products]
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 UniProt Secondary Accession #    Q2I8G2; Q2XN99; Q86TH8; Q9BZK1; B0LPF6 [Other Products]
 UniProt Related Accession #    P08913 [Other Products]
 Molecular Weight    48,957 Da
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 NCBI Official Full Name    alpha-2A adrenergic receptor
 NCBI Official Synonym Full Names    adrenoceptor alpha 2A
 NCBI Official Symbol    ADRA2A [Similar Products]
 NCBI Official Synonym Symbols   
[Similar Products]
 NCBI Protein Information    alpha-2A adrenergic receptor; alpha-2A adrenoceptor; alpha-2AAR subtype C10; alpha-2A adrenoreceptor; adrenergic, alpha-2A-, receptor; alpha-2 adrenergic receptor subtype C10; alpha-2-adrenergic receptor, platelet type
 UniProt Protein Name    Alpha-2A adrenergic receptor
 UniProt Synonym Protein Names   
Alpha-2 adrenergic receptor subtype C10; Alpha-2A adrenoreceptor; Alpha-2A adrenoceptor; Alpha-2AAR
 Protein Family    Alpha-2A adrenergic receptor
 UniProt Gene Name    ADRA2A [Similar Products]
 UniProt Synonym Gene Names    ADRA2R; ADRAR; Alpha-2A adrenoceptor; Alpha-2AAR [Similar Products]
 UniProt Entry Name    ADA2A_HUMAN
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 NCBI Summary for ADRA2A    Alpha-2-adrenergic receptors are members of the G protein-coupled receptor superfamily. They include 3 highly homologous subtypes: alpha2A, alpha2B, and alpha2C. These receptors have a critical role in regulating neurotransmitter release from sympathetic nerves and from adrenergic neurons in the central nervous system. Studies in mouse revealed that both the alpha2A and alpha2C subtypes were required for normal presynaptic control of transmitter release from sympathetic nerves in the heart and from central noradrenergic neurons; the alpha2A subtype inhibited transmitter release at high stimulation frequencies, whereas the alpha2C subtype modulated neurotransmission at lower levels of nerve activity. This gene encodes alpha2A subtype and it contains no introns in either its coding or untranslated sequences. [provided by RefSeq, Jul 2008]
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 UniProt Comments for ADRA2A    ADRA2A: Alpha-2 adrenergic receptors mediate the catecholamine- induced inhibition of adenylate cyclase through the action of G proteins. The rank order of potency for agonists of this receptor is oxymetazoline > clonidine > epinephrine > norepinephrine > phenylephrine > dopamine > p-synephrine > p-tyramine > serotonin = p-octopamine. For antagonists, the rank order is yohimbine > phentolamine = mianserine > chlorpromazine = spiperone = prazosin > propanolol > alprenolol = pindolol. Belongs to the G-protein coupled receptor 1 family. Adrenergic receptor subfamily. ADRA2A sub-subfamily.

Protein type: Membrane protein, integral; Membrane protein, multi-pass; Receptor, GPCR; GPCR, family 1

Chromosomal Location of Human Ortholog: 10q25.2

Cellular Component: integral to plasma membrane; basolateral plasma membrane; cytoplasm; plasma membrane; synapse; receptor complex

Molecular Function: protein binding; protein homodimerization activity; alpha-2C adrenergic receptor binding; protein heterodimerization activity; thioesterase binding; alpha2-adrenergic receptor activity; norepinephrine binding; alpha-1B adrenergic receptor binding; epinephrine binding; protein kinase binding; heterotrimeric G-protein binding

Biological Process: fear response; negative regulation of calcium ion-dependent exocytosis; positive regulation of potassium ion transport; female pregnancy; thermoception; signal transduction; glucose homeostasis; positive regulation of vasodilation; positive regulation of membrane protein ectodomain proteolysis; Rho protein signal transduction; negative regulation of insulin secretion; intestinal absorption; positive regulation of MAP kinase activity; negative regulation of cAMP biosynthetic process; positive regulation of epidermal growth factor receptor activity; negative regulation of lipid catabolic process; positive regulation of cell proliferation; DNA replication; positive regulation of cytokine production; platelet activation; negative regulation of calcium ion transport; activation of protein kinase B; negative regulation of adenylate cyclase activity; cellular response to hormone stimulus; G-protein coupled receptor protein signaling pathway; regulation of vasoconstriction; Ras protein signal transduction; energy reserve metabolic process; activation of protein kinase activity; negative regulation of epinephrine secretion; cell motility; actin cytoskeleton organization and biogenesis; blood coagulation; regulation of insulin secretion; acute inflammatory response; positive regulation of cell migration
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 Research Articles on ADRA2A    1. The ADRA2A gene is associated with withdrawn behavior and reinforces the role of catecholaminergic genes in the heritability of withdrawn behavior.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with ADRA2A elisa kitPathways associated with ADRA2A elisa kit
 Reference Product  PubMed Publications
 POMC elisa kit  >93 publications with ADRA2A and POMC
 NPY elisa kit  >93 publications with ADRA2A and NPY
 RGS4 elisa kit  >7 publications with ADRA2A and RGS4
 ADCY5 elisa kit  >6 publications with ADRA2A and ADCY5
 MTNR1B elisa kit  >3 publications with ADRA2A and MTNR1B
 GNAI2 elisa kit  >2 publications with ADRA2A and GNAI2
 Products by Pathway  Pathway Diagram
 Adrenaline Signalling Through Alpha-2 Adrenergic Receptor Pathway antibodies  Adrenaline Signalling Through Alpha-2 Adrenergic Receptor Pathway Diagram
 Adrenaline,noradrenaline Inhibits Insulin Secretion Pathway antibodies  Adrenaline,noradrenaline Inhibits Insulin Secretion Pathway Diagram
 Adrenoceptors Pathway antibodies  Adrenoceptors Pathway Diagram
 Amine Ligand-binding Receptors Pathway antibodies  Amine Ligand-binding Receptors Pathway Diagram
 Class A/1 (Rhodopsin-like Receptors) Pathway antibodies  Class A/1 (Rhodopsin-like Receptors) Pathway Diagram
 G Alpha (i) Signalling Events Pathway antibodies  G Alpha (i) Signalling Events Pathway Diagram
 G Alpha (z) Signalling Events Pathway antibodies  G Alpha (z) Signalling Events Pathway Diagram
 GPCR Downstream Signaling Pathway antibodies  GPCR Downstream Signaling Pathway Diagram
 GPCR Ligand Binding Pathway antibodies  GPCR Ligand Binding Pathway Diagram
 GPCRs, Class A Rhodopsin-like Pathway antibodies  GPCRs, Class A Rhodopsin-like Pathway Diagram
Diseases associated with ADRA2A elisa kitOrgans/Tissues associated with ADRA2A elisa kit
 Disease Name  Pubmed Publications
 Nervous System Diseases Antibodies  >913 publications with ADRA2A and Nervous System Diseases
 Hypertension Antibodies  >656 publications with ADRA2A and Hypertension
 Pain Antibodies  >524 publications with ADRA2A and Pain
 Disease Models, Animal Antibodies  >290 publications with ADRA2A and Disease Models, Animal
 Heart Diseases Antibodies  >275 publications with ADRA2A and Heart Diseases
 Hypotension Antibodies  >225 publications with ADRA2A and Hypotension
 Depressive Disorder Antibodies  >166 publications with ADRA2A and Depressive Disorder
 Bradycardia Antibodies  >142 publications with ADRA2A and Bradycardia
 Tachycardia Antibodies  >139 publications with ADRA2A and Tachycardia
 Inflammation Antibodies  >107 publications with ADRA2A and Inflammation
 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >3263 publications with ADRA2A and Brain
 Heart Antibodies  >1221 publications with ADRA2A and Heart
 Nerve Antibodies  >1112 publications with ADRA2A and Nerve
 Kidney Antibodies  >578 publications with ADRA2A and Kidney
 Intestine Antibodies  >275 publications with ADRA2A and Intestine
 Eye Antibodies  >250 publications with ADRA2A and Eye
 Connective Tissue Antibodies  >225 publications with ADRA2A and Connective Tissue
 Pancreas Antibodies  >132 publications with ADRA2A and Pancreas
 Adrenal Gland Antibodies  >122 publications with ADRA2A and Adrenal Gland
 Stomach Antibodies  >116 publications with ADRA2A and Stomach
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