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AT-III elisa kit :: Canine Anti-Thrombin III ELISA Kit

Scan QR to view Datasheet Catalog #    MBS743519
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $440 +1 FREE 8GB USB
96-Strip-Wells  /  $640 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,895 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,415 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Anti-Thrombin III (AT-III), ELISA Kit

★Popular Item★
 Also Known As   

Canine Anti-Thrombin III ELISA Kit

 Product Gene Name   

AT-III elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 Species Reactivity    Canine
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 Specificity    This assay has high sensitivity and excellent specificity for detection of AT3. No significant cross-reactivity or interference between AT3 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between AT3 and all the analogues, therefore, cross reaction may still exist in some cases.
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 Samples    Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
 Assay Type    Sandwich
 Detection Range    25-500ng/mL
 Sensitivity    1.0ng/mL
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 Preparation and Storage    Store all reagents at 2-8 degree C.
 Sample Preparation    We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of AT-III elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for AT-III purchase    MBS743519 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Anti-Thrombin III (AT-III) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 25-500ng/mL in biological research samples containing AT-III, with an estimated sensitivity of 1.0ng/mL. The ELISA analytical biochemical technique of the MBS743519 kit is based on AT-III antibody-AT-III antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect AT-III antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, AT-III. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for AT-III elisa kit

   Intended Uses||This AT3 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Canine AT3. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Principle of the Assay: AT3 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-AT3 antibody and an AT3-HRP conjugate. The assay sample and buffer are incubated together with AT3-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the AT3 concentration since AT3 from samples and AT3-HRP conjugate compete for the anti-AT3 antibody binding site. Since the number of sites is limited, as more sites are occupied by AT3 from the sample, fewer sites are left to bind AT3-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The AT3 concentration in each sample is interpolated from this standard curve.
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 Typical Testing Data/Standard Curve (for reference only) of AT-III elisa kit    AT-III elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS743519. Click to request current manual
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NCBI/Uniprot data below describe general gene information for AT-III. It may not necessarily be applicable to this product.
 NCBI GI #    258959
 NCBI GeneID    11905
 NCBI Accession #    AAB23965.1 [Other Products]
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 UniProt Related Accession #    P32261 [Other Products]
 Molecular Weight    52,004 Da
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 NCBI Official Full Name    antithrombin III
 NCBI Official Synonym Full Names    serine (or cysteine) peptidase inhibitor, clade C (antithrombin), member 1
 NCBI Official Symbol    Serpinc1 [Similar Products]
 NCBI Official Synonym Symbols   
At3; At-3; ATIII; AI114908
[Similar Products]
 NCBI Protein Information    antithrombin-III; serpin C1; anti-thrombin 3; serpin peptidase inhibitor, clade C, member 1; serine (or cysteine) proteinase inhibitor, clade C (antithrombin), member 1
 UniProt Protein Name    Antithrombin-III
 UniProt Synonym Protein Names   
Serpin C1
 UniProt Gene Name    Serpinc1 [Similar Products]
 UniProt Synonym Gene Names    At3; ATIII [Similar Products]
 UniProt Entry Name    ANT3_MOUSE
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 UniProt Comments for AT-III    SERPINC1: Most important serine protease inhibitor in plasma that regulates the blood coagulation cascade. AT-III inhibits thrombin, matriptase-3/TMPRSS7, as well as factors IXa, Xa and XIa. Its inhibitory activity is greatly enhanced in the presence of heparin. Defects in SERPINC1 are the cause of antithrombin III deficiency (AT3D). AT3D is an important risk factor for hereditary thrombophilia, a hemostatic disorder characterized by a tendency to recurrent thrombosis. AT3D is classified into 4 types. Type I: characterized by a 50% decrease in antigenic and functional levels. Type II: has defects affecting the thrombin- binding domain. Type III: alteration of the heparin-binding domain. Plasma AT-III antigen levels are normal in type II and III. Type IV: consists of miscellaneous group of unclassifiable mutations. Belongs to the serpin family.

Protein type: Inhibitor; Secreted; Secreted, signal peptide

Cellular Component: extracellular space; extracellular region

Molecular Function: serine-type endopeptidase inhibitor activity; heparin binding; protease binding; protease inhibitor activity

Biological Process: hemostasis; negative regulation of inflammatory response; negative regulation of peptidase activity; blood coagulation; response to nutrient
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 Research Articles on AT-III    1. RNA interference of Serpinc1 and/or Proc allows for evaluation of the function of these genes in vivo and provides a novel, controlled mouse model for spontaneous venous thrombosis.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with AT-III elisa kitPathways associated with AT-III elisa kit
 Reference Product  PubMed Publications
 Plg elisa kit  >56 publications with AT-III and Plg
 F11 elisa kit  >4 publications with AT-III and F11
 Ambp elisa kit  >2 publications with AT-III and Ambp
 F10 elisa kit  >2 publications with AT-III and F10
 Apoh elisa kit  >1 publications with AT-III and Apoh
 Hpx elisa kit  >1 publications with AT-III and Hpx
 Products by Pathway  Pathway Diagram
 Common Pathway antibodies  Common Pathway Diagram
 Complement And Coagulation Cascades Pathway antibodies  Complement And Coagulation Cascades Pathway Diagram
 Complement And Coagulation Cascades Pathway antibodies  Complement And Coagulation Cascades Pathway Diagram
 Complement And Coagulation Cascades Pathway antibodies  Complement And Coagulation Cascades Pathway Diagram
 Formation Of Fibrin Clot (Clotting Cascade) Pathway antibodies  Formation Of Fibrin Clot (Clotting Cascade) Pathway Diagram
 Hemostasis Pathway antibodies  Hemostasis Pathway Diagram
Diseases associated with AT-III elisa kitOrgans/Tissues associated with AT-III elisa kit
 Disease Name  Pubmed Publications
 Vascular Diseases Antibodies  >3814 publications with AT-III and Vascular Diseases
 Thrombosis Antibodies  >3234 publications with AT-III and Thrombosis
 Blood Coagulation Disorders Antibodies  >2693 publications with AT-III and Blood Coagulation Disorders
 Antithrombin III Deficiency Antibodies  >1853 publications with AT-III and Antithrombin III Deficiency
 Venous Thrombosis Antibodies  >1337 publications with AT-III and Venous Thrombosis
 Thromboembolism Antibodies  >1083 publications with AT-III and Thromboembolism
 Disseminated Intravascular Coagulation Antibodies  >890 publications with AT-III and Disseminated Intravascular Coagulation
 Inflammation Antibodies  >722 publications with AT-III and Inflammation
 Hemorrhage Antibodies  >617 publications with AT-III and Hemorrhage
 Liver Diseases Antibodies  >516 publications with AT-III and Liver Diseases
 Organ/Tissue Name  Pubmed Publications
 Liver Antibodies  >876 publications with AT-III and Liver
 Lung Antibodies  >348 publications with AT-III and Lung
 Muscle Antibodies  >139 publications with AT-III and Muscle
 Ovary Antibodies  >63 publications with AT-III and Ovary
 Embryonic Tissue Antibodies  >60 publications with AT-III and Embryonic Tissue
 Pancreas Antibodies  >42 publications with AT-III and Pancreas
 Stomach Antibodies  >35 publications with AT-III and Stomach
 Salivary Gland Antibodies  >6 publications with AT-III and Salivary Gland
 Thymus Antibodies  >4 publications with AT-III and Thymus
 Uncharacterized Tissue Antibodies  >1 publications with AT-III and Uncharacterized Tissue
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