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bFGF elisa kit :: Canine Basic Fibroblast growth factor ELISA Kit

Scan QR to view Datasheet Catalog #    MBS741542
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $455 +1 FREE 8GB USB
96-Strip-Wells  /  $660 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,925 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,490 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Basic Fibroblast growth factor (bFGF), ELISA Kit

★Popular Item★
 Also Known As   

Canine Basic Fibroblast growth factor ELISA Kit

 Product Gene Name   

bFGF elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    134920
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 Species Reactivity    Canine
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 Specificity    This assay has high sensitivity and excellent specificity for detection of BFGF. No significant cross-reactivity or interference between BFGF and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between BFGF and all the analogues, therefore, cross reaction may still exist in some cases.
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 Samples    Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
 Assay Type    Sandwich
 Detection Range    50-1000pg/mL
 Sensitivity    1.0pg/mL
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 Preparation and Storage    Store all reagents at 2-8 degree C.
 Sample Preparation    We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of bFGF elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for bFGF purchase    MBS741542 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Basic Fibroblast growth factor (bFGF) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 50-1000pg/mL in biological research samples containing bFGF, with an estimated sensitivity of 1.0pg/mL. The ELISA analytical biochemical technique of the MBS741542 kit is based on bFGF antibody-bFGF antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect bFGF antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, bFGF. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for bFGF elisa kit

   Intended Uses: This BFGF ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Canine BFGF.

Principle of the Assay: BFGF ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for BFGF. Standards or samples are then added to the microtiter plate wells and BFGF if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of BFGF present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for BFGF are added to each well to "sandwich" the BFGF immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain BFGF and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The BFGF concentration in each sample is interpolated from this standard curve.
 Product Categories/Family for bFGF elisa kit    Signal Transduction
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 Typical Testing Data/Standard Curve (for reference only) of bFGF elisa kit    bFGF elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS741542. Click to request current manual
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NCBI/Uniprot data below describe general gene information for bFGF. It may not necessarily be applicable to this product.
 NCBI GI #    8250666
 NCBI GeneID    2247
 NCBI Accession #    AAB21432.2 [Other Products]
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 UniProt Secondary Accession #    O00527; P78443; Q16443; Q5PY50; Q7KZ11; Q7KZ72; Q9UC54; Q9UCS5; Q9UCS6; A4LBB8 [Other Products]
 UniProt Related Accession #    P09038 [Other Products]
 Molecular Weight    21,203 Da
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 NCBI Official Full Name    basic fibroblast growth factor, partial
 NCBI Official Synonym Full Names    fibroblast growth factor 2 (basic)
 NCBI Official Symbol    FGF2 [Similar Products]
 NCBI Official Synonym Symbols   
BFGF; FGFB; FGF-2; HBGF-2
[Similar Products]
 NCBI Protein Information    fibroblast growth factor 2; prostatropin; heparin-binding growth factor 2; basic fibroblast growth factor bFGF
 UniProt Protein Name    Fibroblast growth factor 2
 UniProt Synonym Protein Names   
Basic fibroblast growth factor; bFGF; Heparin-binding growth factor 2; HBGF-2
 UniProt Gene Name    FGF2 [Similar Products]
 UniProt Synonym Gene Names    FGFB; FGF-2; bFGF; HBGF-2 [Similar Products]
 UniProt Entry Name    FGF2_HUMAN
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 NCBI Summary for bFGF    The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members bind heparin and possess broad mitogenic and angiogenic activities. This protein has been implicated in diverse biological processes, such as limb and nervous system development, wound healing, and tumor growth. The mRNA for this gene contains multiple polyadenylation sites, and is alternatively translated from non-AUG (CUG) and AUG initiation codons, resulting in five different isoforms with distinct properties. The CUG-initiated isoforms are localized in the nucleus and are responsible for the intracrine effect, whereas, the AUG-initiated form is mostly cytosolic and is responsible for the paracrine and autocrine effects of this FGF. [provided by RefSeq, Jul 2008]
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 UniProt Comments for bFGF    FGF2: Plays an important role in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. Functions as potent mitogen in vitro. Monomer. Homodimer. Interacts with FGFR1, FGFR2, FGFR3 and FGFR4. Affinity between fibroblast growth factors (FGFs) and their receptors is increased by heparan sulfate glycosaminoglycans that function as coreceptors. Interacts with CSPG4, FGFBP1 and TEC. Found in a complex with FGFBP1, FGF1 and FGF2. Expressed in granulosa and cumulus cells. Expressed in hepatocellular carcinoma cells, but not in non- cancerous liver tissue. Belongs to the heparin-binding growth factors family. 4 isoforms of the human protein are produced by alternative initiation.

Protein type: Motility/polarity/chemotaxis; Activator

Chromosomal Location of Human Ortholog: 4q26

Cellular Component: extracellular space; extracellular region; nucleus

Molecular Function: heparin binding; protein binding; growth factor activity; ligand-dependent nuclear receptor transcription coactivator activity; cytokine activity; chemoattractant activity; fibroblast growth factor receptor binding

Biological Process: extracellular matrix organization and biogenesis; wound healing; nerve growth factor receptor signaling pathway; activation of MAPK activity; somatic stem cell maintenance; positive regulation of transcription, DNA-dependent; chemotaxis; signal transduction; hyaluronan catabolic process; growth factor dependent regulation of satellite cell proliferation; positive regulation of MAP kinase activity; positive chemotaxis; positive regulation of cell proliferation; embryonic morphogenesis; positive regulation of cardiac muscle cell proliferation; epidermal growth factor receptor signaling pathway; nervous system development; phosphoinositide-mediated signaling; fibroblast growth factor receptor signaling pathway; positive regulation of cell fate specification; positive regulation of blood vessel endothelial cell migration; positive regulation of phosphoinositide 3-kinase activity; negative regulation of blood vessel endothelial cell migration; regulation of angiogenesis; organ morphogenesis; positive regulation of angiogenesis; cell migration during sprouting angiogenesis; ureteric bud branching; positive regulation of cell division; release of sequestered calcium ion into cytosol; Ras protein signal transduction; phosphatidylinositol biosynthetic process; insulin receptor signaling pathway; innate immune response; positive regulation of transcription from RNA polymerase II promoter; positive regulation of endothelial cell proliferation; inositol phosphate biosynthetic process
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 Research Articles on bFGF    1. Identification of critical factors needed for reprogramming may allow for nonviral, chemically defined derivation of human-induced multipotent cells that can be maintained by exogenous FGF2.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Pathways associated with bFGF elisa kitDiseases associated with bFGF elisa kit
 Products by Pathway  Pathway Diagram
 Activated Point Mutants Of FGFR2 Pathway antibodies  Activated Point Mutants Of FGFR2 Pathway Diagram
 Adaptive Immune System Pathway antibodies  Adaptive Immune System Pathway Diagram
 Angiogenesis Pathway antibodies  Angiogenesis Pathway Diagram
 Angiopoietin Receptor Tie2-mediated Signaling Pathway antibodies  Angiopoietin Receptor Tie2-mediated Signaling Pathway Diagram
 Cardiac Progenitor Differentiation Pathway antibodies  Cardiac Progenitor Differentiation Pathway Diagram
 Constitutive PI3K/AKT Signaling In Cancer Pathway antibodies  Constitutive PI3K/AKT Signaling In Cancer Pathway Diagram
 DAP12 Interactions Pathway antibodies  DAP12 Interactions Pathway Diagram
 DAP12 Signaling Pathway antibodies  DAP12 Signaling Pathway Diagram
 Developmental Biology Pathway antibodies  Developmental Biology Pathway Diagram
 Disease Pathway antibodies  Disease Pathway Diagram
 Disease Name  Pubmed Publications
 Nervous System Diseases Antibodies  >974 publications with bFGF and Nervous System Diseases
 Necrosis Antibodies  >603 publications with bFGF and Necrosis
 Wounds and Injuries Antibodies  >552 publications with bFGF and Wounds and Injuries
 Inflammation Antibodies  >426 publications with bFGF and Inflammation
 Heart Diseases Antibodies  >413 publications with bFGF and Heart Diseases
 Myocardial Ischemia Antibodies  >343 publications with bFGF and Myocardial Ischemia
 Disease Progression Antibodies  >331 publications with bFGF and Disease Progression
 Lung Diseases Antibodies  >311 publications with bFGF and Lung Diseases
 Hyperplasia Antibodies  >254 publications with bFGF and Hyperplasia
 Glioma Antibodies  >223 publications with bFGF and Glioma
Organs/Tissues associated with bFGF elisa kit
 Organ/Tissue Name  Pubmed Publications
 Vascular Antibodies  >4634 publications with bFGF and Vascular
 Brain Antibodies  >2154 publications with bFGF and Brain
 Bone Antibodies  >1773 publications with bFGF and Bone
 Muscle Antibodies  >1771 publications with bFGF and Muscle
 Embryonic Tissue Antibodies  >1260 publications with bFGF and Embryonic Tissue
 Eye Antibodies  >1016 publications with bFGF and Eye
 Skin Antibodies  >813 publications with bFGF and Skin
 Connective Tissue Antibodies  >684 publications with bFGF and Connective Tissue
 Heart Antibodies  >644 publications with bFGF and Heart
 Bone Marrow Antibodies  >636 publications with bFGF and Bone Marrow
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