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BATF3 elisa kit :: Canine Basic leucine zipper transcriptional factor ATF-like 3 (BATF3) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS7210858
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $470 +1 FREE 8GB USB
96-Strip-Wells  /  $675 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,925 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,490 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Basic leucine zipper transcriptional factor ATF-like 3 (BATF3), ELISA Kit

 Also Known As   

Canine Basic leucine zipper transcriptional factor ATF-like 3 (BATF3) ELISA Kit

 Product Gene Name   

BATF3 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Manual Insert    Download PDF Manual View PDF Manual
 OMIM    612470
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 Species Reactivity    Canine
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 Specificity    This assay has high sensitivity and excellent specificity for detection of BATF3. No significant cross-reactivity or interference between BATF3 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between BATF3 and all the analogues, therefore, cross reaction may still exist in some cases.
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 Samples    Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
 Assay Type    Competitive
 Sensitivity    0.1 ng/mL.
 Intended Uses    This BATF3 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Canine BATF3. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
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 Preparation and Storage    Store all reagents at 2-8 degree C.
 Sample Preparation    We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
 Product Note    Our ELISA assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay.
 Other Notes    Small volumes of BATF3 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for BATF3 purchase    MBS7210858 is a ready-to-use microwell, strip plate Competitive ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Basic leucine zipper transcriptional factor ATF-like 3 (BATF3) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing BATF3, with an estimated sensitivity of 0.1 ng/mL. The ELISA analytical biochemical technique of the MBS7210858 kit is based on BATF3 antibody-BATF3 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect BATF3 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, BATF3. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Product Description specifically for BATF3 elisa kit

   Principle of the assay: BATF3 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-BATF3 antibody and an BATF3-HRP conjugate. The assay sample and buffer are incubated together with BATF3-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the BATF3 concentration since BATF3 from samples and BATF3-HRP conjugate compete for the anti-BATF3 antibody binding site. Since the number of sites is limited, as more sites are occupied by BATF3 from the sample, fewer sites are left to bind BATF3-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The BATF3 concentration in each sample is interpolated from this standard curve.
 Product Categories/Family for BATF3 elisa kit    Epigenetics and Nuclear Signaling
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 Typical Testing Data/Standard Curve (for reference only) of BATF3 elisa kit    BATF3 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS7210858
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NCBI/Uniprot data below describe general gene information for BATF3. It may not necessarily be applicable to this product.
 NCBI GI #    8924246
 NCBI GeneID    55509
 NCBI Accession #    NP_061134.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_018664.2 [Other Products]
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 UniProt Related Accession #    Q9NR55 [Other Products]
 Molecular Weight    14,468 Da
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 NCBI Official Full Name    basic leucine zipper transcriptional factor ATF-like 3
 NCBI Official Synonym Full Names    basic leucine zipper transcription factor, ATF-like 3
 NCBI Official Symbol    BATF3 [Similar Products]
 NCBI Official Synonym Symbols   
JDP1; SNFT; JUNDM1
[Similar Products]
 NCBI Protein Information    basic leucine zipper transcriptional factor ATF-like 3; B-ATF-3; Jun dimerization protein 1; Jun dimerization protein p21SNFT; 21-kD small nuclear factor isolated from T cells; 21 kDa small nuclear factor isolated from T-cells
 UniProt Protein Name    Basic leucine zipper transcriptional factor ATF-like 3
 UniProt Synonym Protein Names   
21 kDa small nuclear factor isolated from T-cells; Jun dimerization protein p21SNFT
 Protein Family    Basic leucine zipper transcriptional factor
 UniProt Gene Name    BATF3 [Similar Products]
 UniProt Synonym Gene Names    SNFT; B-ATF-3 [Similar Products]
 UniProt Entry Name    BATF3_HUMAN
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 NCBI Summary for BATF3    This gene encodes a member of the basic leucine zipper protein family. The encoded protein functions as a transcriptional repressor when heterodimerizing with JUN. The protein may play a role in repression of interleukin-2 and matrix metalloproteinase-1 transcription.[provided by RefSeq, Feb 2009]
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 UniProt Comments for BATF3    BATF3: Functions as a negative regulator of AP-1-mediated transcription by heterodimerizing with JUN and binding DNA at 12- O-tetradecanoylphorbol-13-acetate response elements (TRE) (consensus: 5'-TGA[CG]TCA-3'). Represses IL2 and MMP1 promoter activities. Belongs to the bZIP family.

Protein type: DNA-binding

Chromosomal Location of Human Ortholog: 1q32.3

Cellular Component: nucleus

Molecular Function: transcription corepressor activity; transcription factor activity

Biological Process: transcription from RNA polymerase II promoter; response to virus; myeloid dendritic cell differentiation; negative regulation of transcription from RNA polymerase II promoter
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 Research Articles on BATF3    1. overexpression of p21SNFT in HepG2 cells leads to repression of matrix metalloproteinase-1 by 70-80%
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with BATF3 elisa kitPathways associated with BATF3 elisa kit
 Reference Product  PubMed Publications
 JUN elisa kit  >9 publications with BATF3 and JUN
 FOS elisa kit  >2 publications with BATF3 and FOS
 Products by Pathway  Pathway Diagram
 Calcineurin-regulated NFAT-dependent Transcription In Lymphocytes Pathway antibodies  Calcineurin-regulated NFAT-dependent Transcription In Lymphocytes Pathway Diagram
 Calcium Signaling In The CD4+ TCR Pathway antibodies  Calcium Signaling In The CD4+ TCR Pathway Diagram
Diseases associated with BATF3 elisa kitOrgans/Tissues associated with BATF3 elisa kit
 Disease Name  Pubmed Publications
 Nervous System Diseases Antibodies  >4 publications with BATF3 and Nervous System Diseases
 Disease Models, Animal Antibodies  >4 publications with BATF3 and Disease Models, Animal
 Inflammation Antibodies  >4 publications with BATF3 and Inflammation
 Necrosis Antibodies  >2 publications with BATF3 and Necrosis
 Lung Diseases Antibodies  >1 publications with BATF3 and Lung Diseases
 Pregnancy Complications Antibodies  >1 publications with BATF3 and Pregnancy Complications
 Liver Diseases Antibodies  >1 publications with BATF3 and Liver Diseases
 Organ/Tissue Name  Pubmed Publications
 Lung Antibodies  >5 publications with BATF3 and Lung
 Thymus Antibodies  >2 publications with BATF3 and Thymus
 Brain Antibodies  >1 publications with BATF3 and Brain
 Connective Tissue Antibodies  >1 publications with BATF3 and Connective Tissue
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