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CFD elisa kit :: Canine Complement Factor D ELISA Kit

Scan QR to view Datasheet Catalog #    MBS018970 CFD elisa kit
Unit / Price
48-Strip-Wells  /  $470 +1 FREE 8GB USB
96-Strip-Wells  /  $680 +1 FREE 8GB USB
5x96-Strip-Wells  /  $3,100 +3 FREE 8GB USB
10x96-Strip-Wells  /  $6,095 +7 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Complement Factor D (CFD), ELISA Kit

★Popular Item★
 Also Known As   

Canine Complement Factor D ELISA Kit

 Product Gene Name   

CFD elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 Chromosome Location    Chromosome: 19; NC_000019.9 (859665..863624). Location: 19p13.3
 OMIM    134350
 3D Structure    ModBase 3D Structure for P00746
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 Species Reactivity    Canine
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 Specificity    No significant cross-reactivity or interference between Canine CFD and analogues was observed.
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 Samples    Serum, Plasma, Tissue Homogenate, Feces, Urine and Body Fluids
 Assay Type    Sandwich
 Detection Range    0.25 ug/ml - 8 ug/ml.
 Sensitivity    0.1 ug/ml.
 Intended Uses    This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated CFD concentrations in Canine serum, plasma and other body fluids. Using Purified Canine CFD antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add CFD and CFD antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of CFD in the samples is then determined by comparing the O.D. of the samples to the standard curve.
 Intra-assay Precision    Intra-assay CV (%) is less than 15%.
 Inter-assay Precision    Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean 100]
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 Preparation and Storage    Store all reagents at 2-8 degree C
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of CFD elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for CFD purchase    MBS018970 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Complement Factor D (CFD) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 0.25 ug/ml - 8 ug/ml in biological research samples containing CFD, with an estimated sensitivity of 0.1 ug/ml. The ELISA analytical biochemical technique of the MBS018970 kit is based on CFD antibody-CFD antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect CFD antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, CFD. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, Plasma, Tissue Homogenate, Feces, Urine and Body Fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for CFD elisa kit

   Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated CFD concentrations in Canine serum, plasma and other body fluids. Using Purified Canine CFD antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add CFD and CFD antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of CFD in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Sample Manual Insert of MBS018970. Click to request current manual
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NCBI/Uniprot data below describe general gene information for CFD. It may not necessarily be applicable to this product.
 NCBI GI #    42544239
 NCBI GeneID    1675
 NCBI Accession #    NP_001919.2 [Other Products]
 NCBI GenBank Nucleotide #    NM_001928.2 [Other Products]
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 UniProt Primary Accession #    P00746 [Other Products]
 UniProt Secondary Accession #    Q5U5S1; Q86VJ5; Q8N4E0; Q8WZB4; B4DV76 [Other Products]
 UniProt Related Accession #    P00746 [Other Products]
 Molecular Weight    27,033 Da
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 NCBI Official Full Name    complement factor D preproprotein
 NCBI Official Synonym Full Names    complement factor D (adipsin)
 NCBI Official Symbol    CFD [Similar Products]
 NCBI Official Synonym Symbols   
DF; ADN; PFD; ADIPSIN
[Similar Products]
 NCBI Protein Information    complement factor D; properdin factor D; C3 convertase activator; complement factor D preproprotein; D component of complement (adipsin)
 UniProt Protein Name    Complement factor D
 UniProt Synonym Protein Names   
Adipsin; C3 convertase activator; Properdin factor D
 Protein Family    Complement factor
 UniProt Gene Name    CFD [Similar Products]
 UniProt Synonym Gene Names    DF; PFD [Similar Products]
 UniProt Entry Name    CFAD_HUMAN
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 NCBI Summary for CFD    The protein encoded by this gene is a member of the trypsin family of peptidases. The encoded protein is a component of the alternative complement pathway best known for its role in humoral suppression of infectious agents. This protein is also a serine protease that is secreted by adipocytes into the bloodstream. Finally, the encoded protein has a high level of expression in fat, suggesting a role for adipose tissue in immune system biology. [provided by RefSeq, Jul 2008]
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 UniProt Comments for CFD    CFD: Factor D cleaves factor B when the latter is complexed with factor C3b, activating the C3bbb complex, which then becomes the C3 convertase of the alternate pathway. Its function is homologous to that of C1s in the classical pathway. Defects in CFD are the cause of complement factor D deficiency (CFDD). CFDD is an immunologic disorder characterized by increased susceptibility to bacterial infections, particularly Neisseria infections, due to a defect in the alternative complement pathway. Belongs to the peptidase S1 family.

Protein type: Secreted, signal peptide; Protease; EC 3.4.21.46; Secreted

Chromosomal Location of Human Ortholog: 19p13.3

Cellular Component: extracellular space; extracellular region

Molecular Function: serine-type peptidase activity; serine-type endopeptidase activity

Biological Process: platelet activation; platelet degranulation; complement activation, alternative pathway; innate immune response; blood coagulation; proteolysis; complement activation

Disease: Complement Factor D Deficiency
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 Research Articles on CFD    1. Increased activation of the alternative complement pathway in vitreous was controlled by disease stage and genetic variation in the complement pathway, supporting a role for complement activation in macular degeneration disease pathogenesis.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Pathways associated with CFD elisa kitDiseases associated with CFD elisa kit
 Products by Pathway  Pathway Diagram
 Adipogenesis Pathway antibodies  Adipogenesis Pathway Diagram
 Alternative Complement Activation Pathway antibodies  Alternative Complement Activation Pathway Diagram
 Complement And Coagulation Cascades Pathway antibodies  Complement And Coagulation Cascades Pathway Diagram
 Complement And Coagulation Cascades Pathway antibodies  Complement And Coagulation Cascades Pathway Diagram
 Complement Cascade Pathway antibodies  Complement Cascade Pathway Diagram
 Hemostasis Pathway antibodies  Hemostasis Pathway Diagram
 Immune System Pathway antibodies  Immune System Pathway Diagram
 Initial Triggering Of Complement Pathway antibodies  Initial Triggering Of Complement Pathway Diagram
 Innate Immune System Pathway antibodies  Innate Immune System Pathway Diagram
 Platelet Activation, Signaling And Aggregation Pathway antibodies  Platelet Activation, Signaling And Aggregation Pathway Diagram
 Disease Name  Pubmed Publications
 Cardiovascular Diseases Antibodies  >285 publications with CFD and Cardiovascular Diseases
 Nervous System Diseases Antibodies  >149 publications with CFD and Nervous System Diseases
 Brain Diseases Antibodies  >128 publications with CFD and Brain Diseases
 Heart Diseases Antibodies  >78 publications with CFD and Heart Diseases
 Hyperplasia Antibodies  >29 publications with CFD and Hyperplasia
 Drug Toxicity Antibodies  >19 publications with CFD and Drug Toxicity
 Disease Models, Animal Antibodies  >15 publications with CFD and Disease Models, Animal
 Inflammation Antibodies  >12 publications with CFD and Inflammation
 Hypertension Antibodies  >12 publications with CFD and Hypertension
 Liver Diseases Antibodies  >9 publications with CFD and Liver Diseases
Organs/Tissues associated with CFD elisa kit
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >589 publications with CFD and Blood
 Vascular Antibodies  >351 publications with CFD and Vascular
 Heart Antibodies  >225 publications with CFD and Heart
 Lung Antibodies  >88 publications with CFD and Lung
 Brain Antibodies  >66 publications with CFD and Brain
 Bone Antibodies  >53 publications with CFD and Bone
 Mouth Antibodies  >42 publications with CFD and Mouth
 Liver Antibodies  >23 publications with CFD and Liver
 Muscle Antibodies  >23 publications with CFD and Muscle
 Pharynx Antibodies  >20 publications with CFD and Pharynx
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