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EGF elisa kit :: Canine Epidermal Growth Factor ELISA Kit

Scan QR to view Datasheet Catalog #    MBS2605242
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $300 +1 FREE 8GB USB
96-Strip-Wells  /  $475 +1 FREE 8GB USB
5x96-Strip-Wells  /  $1,825 +2 FREE 8GB USB
10x96-Strip-Wells  /  $3,425 +4 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Epidermal Growth Factor (EGF), ELISA Kit

★Popular Item★
 Also Known As   

Canine Epidermal Growth Factor (EGF) ELISA Kit

 Product Gene Name   

EGF elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    131530
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 Species Reactivity    Canine
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 Specificity    No cross-reaction with other factors.
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 Samples    Serum, plasma or cell culture supernatant and organizations in the natural and recombinant EGF concentration.
 Assay Type    Sandwich
 Detection Range    1000 pg/ml-15.6 pg/ml
 Sensitivity    5 pg/ml.
 Intra-assay Precision    <= 8%
 Inter-assay Precision    <= 12%
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 Preparation and Storage    Store all reagents at 2-8 degree C.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of EGF elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for EGF purchase    MBS2605242 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Epidermal Growth Factor (EGF) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 1000 pg/ml-15.6 pg/ml in biological research samples containing EGF, with an estimated sensitivity of 5 pg/ml. The ELISA analytical biochemical technique of the MBS2605242 kit is based on EGF antibody-EGF antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect EGF antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, EGF. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma or cell culture supernatant and organizations in the natural and recombinant EGF concentration. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for EGF elisa kit

   Principle of the Assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Canine EGF monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.
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 Typical Testing Data/Standard Curve (for reference only) of EGF elisa kit    EGF elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS2605242. Click to request current manual
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NCBI/Uniprot data below describe general gene information for EGF. It may not necessarily be applicable to this product.
 NCBI GI #    374922206
 NCBI GeneID    1950
 NCBI Accession #    AFA26280.1 [Other Products]
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 UniProt Secondary Accession #    Q52LZ6; B4DRK7; E7EVD2; E9PBF0 [Other Products]
 UniProt Related Accession #    P01133 [Other Products]
 Molecular Weight    129,746 Da
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 NCBI Official Full Name    epidermal growth factor, partial
 NCBI Official Synonym Full Names    epidermal growth factor
 NCBI Official Symbol    EGF [Similar Products]
 NCBI Official Synonym Symbols   
URG; HOMG4
[Similar Products]
 NCBI Protein Information    pro-epidermal growth factor
 UniProt Protein Name    Pro-epidermal growth factor
 UniProt Synonym Protein Names   
Epidermal growth factorAlternative name(s):Urogastrone
 Protein Family    Epidermal growth factor
 UniProt Gene Name    EGF [Similar Products]
 UniProt Synonym Gene Names    EGF [Similar Products]
 UniProt Entry Name    EGF_HUMAN
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 NCBI Summary for EGF    This gene encodes a member of the epidermal growth factor superfamily. The encoded preproprotein is proteolytically processed to generate the 53-amino acid epidermal growth factor peptide. This protein acts a potent mitogenic factor that plays an important role in the growth, proliferation and differentiation of numerous cell types. This protein acts by binding with high affinity to the cell surface receptor, epidermal growth factor receptor. Defects in this gene are the cause of hypomagnesemia type 4. Dysregulation of this gene has been associated with the growth and progression of certain cancers. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed. [provided by RefSeq, Jan 2016]
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 UniProt Comments for EGF    EGF: EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. Magnesiotropic hormone that stimulates magnesium reabsorption in the renal distal convoluted tubule via engagement of EGFR and activation of the magnesium channel TRPM6. Defects in EGF are the cause of hypomagnesemia type 4 (HOMG4); also known as renal hypomagnesemia normocalciuric. HOMG4 is a disorder characterized by massive renal hypomagnesemia and normal levels of serum calcium and calcium excretion. Clinical features include seizures, mild-to mederate psychomotor retardation, and brisk tendon reflexes. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Hormone; Ligand, receptor tyrosine kinase; Membrane protein, integral

Chromosomal Location of Human Ortholog: 4q25

Cellular Component: extracellular region; extracellular space; lysosomal membrane; receptor complex

Molecular Function: epidermal growth factor receptor binding; growth factor activity; phosphatidylinositol-4,5-bisphosphate 3-kinase activity; protein binding; protein-tyrosine kinase activity; Ras guanyl-nucleotide exchange factor activity; transmembrane receptor protein tyrosine kinase activator activity; Wnt receptor activity; Wnt-protein binding

Biological Process: angiogenesis; DNA replication; epidermal growth factor receptor signaling pathway; MAPKKK cascade; negative regulation of epidermal growth factor receptor signaling pathway; negative regulation of secretion; phosphoinositide-mediated signaling; platelet degranulation; positive regulation of cell proliferation; positive regulation of DNA binding; positive regulation of epidermal growth factor receptor activity; positive regulation of MAP kinase activity; positive regulation of mitosis; positive regulation of phosphorylation; positive regulation of receptor internalization; positive regulation of transcription, DNA-dependent; regulation of phosphoinositide 3-kinase cascade; signal transduction; STAT protein nuclear translocation; Wnt receptor signaling pathway through beta-catenin

Disease: Hypomagnesemia 4, Renal
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 Research Articles on EGF    1. The findings of this study provide evidence that multiple sclerosis spinal cord simultaneously lack Cbl, EGF, and PrPCs.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with EGF elisa kitPathways associated with EGF elisa kit
 Reference Product  PubMed Publications
 EGFR elisa kit  >6067 publications with EGF and EGFR
 ERBB2 elisa kit  >1392 publications with EGF and ERBB2
 HBEGF elisa kit  >1110 publications with EGF and HBEGF
 GRB2 elisa kit  >375 publications with EGF and GRB2
 ERBB4 elisa kit  >329 publications with EGF and ERBB4
 ERBB3 elisa kit  >298 publications with EGF and ERBB3
 NRG1 elisa kit  >96 publications with EGF and NRG1
 ADAM12 elisa kit  >28 publications with EGF and ADAM12
 SERPINE1 elisa kit  >10 publications with EGF and SERPINE1
 ZFYVE28 elisa kit  >1 publications with EGF and ZFYVE28
 Products by Pathway  Pathway Diagram
 ARMS-mediated Activation Pathway antibodies  ARMS-mediated Activation Pathway Diagram
 Adaptive Immune System Pathway antibodies  Adaptive Immune System Pathway Diagram
 Arf6 Signaling Events Pathway antibodies  Arf6 Signaling Events Pathway Diagram
 Axon Guidance Pathway antibodies  Axon Guidance Pathway Diagram
 Bladder Cancer Pathway antibodies  Bladder Cancer Pathway Diagram
 Bladder Cancer Pathway antibodies  Bladder Cancer Pathway Diagram
 Ceramide Signaling Pathway antibodies  Ceramide Signaling Pathway Diagram
 Choline Metabolism In Cancer Pathway antibodies  Choline Metabolism In Cancer Pathway Diagram
 Choline Metabolism In Cancer Pathway antibodies  Choline Metabolism In Cancer Pathway Diagram
 Constitutive Signaling By Aberrant PI3K In Cancer Pathway antibodies  Constitutive Signaling By Aberrant PI3K In Cancer Pathway Diagram
Diseases associated with EGF elisa kitOrgans/Tissues associated with EGF elisa kit
 Disease Name  Pubmed Publications
 Breast Neoplasms Antibodies  >1776 publications with EGF and Breast Neoplasms
 Adenocarcinoma Antibodies  >1395 publications with EGF and Adenocarcinoma
 Necrosis Antibodies  >1046 publications with EGF and Necrosis
 Neoplasms, Experimental Antibodies  >929 publications with EGF and Neoplasms, Experimental
 Lung Neoplasms Antibodies  >925 publications with EGF and Lung Neoplasms
 Cell Transformation, Neoplastic Antibodies  >907 publications with EGF and Cell Transformation, Neoplastic
 Inflammation Antibodies  >822 publications with EGF and Inflammation
 Neoplasm Metastasis Antibodies  >706 publications with EGF and Neoplasm Metastasis
 Liver Diseases Antibodies  >621 publications with EGF and Liver Diseases
 Neoplasm Invasiveness Antibodies  >614 publications with EGF and Neoplasm Invasiveness
 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >1971 publications with EGF and Brain
 Lung Antibodies  >1943 publications with EGF and Lung
 Kidney Antibodies  >1658 publications with EGF and Kidney
 Skin Antibodies  >1486 publications with EGF and Skin
 Muscle Antibodies  >1398 publications with EGF and Muscle
 Embryonic Tissue Antibodies  >1166 publications with EGF and Embryonic Tissue
 Eye Antibodies  >975 publications with EGF and Eye
 Intestine Antibodies  >939 publications with EGF and Intestine
 Mouth Antibodies  >896 publications with EGF and Mouth
 Connective Tissue Antibodies  >485 publications with EGF and Connective Tissue
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