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gGT1 elisa kit :: Canine Gamma Glutamyltransferase 1 ELISA Kit

Scan QR to view Datasheet Catalog #    MBS739358
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $470 +1 FREE 8GB USB
96-Strip-Wells  /  $675 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,925 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,490 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Gamma Glutamyltransferase 1 (gGT1), ELISA Kit

 Also Known As   

Canine Gamma Glutamyltransferase 1 ELISA Kit

 Product Gene Name   

gGT1 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    612346
 3D Structure    ModBase 3D Structure for P19440
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 Species Reactivity    Canine
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 Samples    Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
 Assay Type    Competitive
 Intended Uses    This gammaGT-1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Canine gammaGT-1. This ELISA kit for research use only, not for therapeutic or diagnostic applications
 !Intended Uses    This gammaGT-1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Canine gammaGT-1. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
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 Preparation and Storage    Store all reagents at 2-8 degree C.
 Sample Preparation    We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
 Product Note    Our ELISA assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of gGT1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for gGT1 purchase    MBS739358 is a ready-to-use microwell, strip plate Competitive ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Gamma Glutamyltransferase 1 (gGT1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing gGT1. The ELISA analytical biochemical technique of the MBS739358 kit is based on gGT1 antibody-gGT1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect gGT1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, gGT1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Product Description specifically for gGT1 elisa kit

   Principle of the Assay: gammaGT-1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-gammaGT-1 antibody and an gammaGT-1-HRP conjugate. The assay sample and buffer are incubated together with gammaGT-1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the gammaGT-1 concentration since gammaGT-1 from samples and gammaGT-1-HRP conjugate compete for the anti-gammaGT-1 antibody binding site. Since the number of sites is limited, as more sites are occupied by gammaGT-1 from the sample, fewer sites are left to bind gammaGT-1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The gammaGT-1 concentration in each sample is interpolated from this standard curve.
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 Typical Testing Data/Standard Curve (for reference only) of gGT1 elisa kit    gGT1 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS739358. Click to request current manual
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NCBI/Uniprot data below describe general gene information for gGT1. It may not necessarily be applicable to this product.
 NCBI GI #    47678519
 NCBI GeneID    2678
 NCBI Accession #    CAG30380.1 [Other Products]
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 UniProt Primary Accession #    P19440 [Other Products]
 UniProt Secondary Accession #    Q08247; Q14404; Q8TBS1; Q9UMK1 [Other Products]
 UniProt Related Accession #    P19440 [Other Products]
 Molecular Weight    24,080 Da
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 NCBI Official Full Name    GGT1
 NCBI Official Synonym Full Names    gamma-glutamyltransferase 1
 NCBI Official Symbol    GGT1 [Similar Products]
 NCBI Official Synonym Symbols   
GGT; GTG; CD224; GGT 1; D22S672; D22S732
[Similar Products]
 NCBI Protein Information    gamma-glutamyltranspeptidase 1; glutathione hydrolase 1; leukotriene-C4 hydrolase
 UniProt Protein Name    Gamma-glutamyltranspeptidase 1
 UniProt Synonym Protein Names   
Gamma-glutamyltransferase 1; Glutathione hydrolase 1 (EC:3.4.19.13); Leukotriene-C4 hydrolase (EC:3.4.19.14); CD_antigen: CD224Cleaved into the following 2 chains:Gamma-glutamyltranspeptidase 1 heavy chain; Gamma-glutamyltranspeptidase 1 light chain
 Protein Family    Gamma-glutamyltranspeptidase
 UniProt Gene Name    GGT1 [Similar Products]
 UniProt Synonym Gene Names    GGT; GGT 1 [Similar Products]
 UniProt Entry Name    GGT1_HUMAN
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 NCBI Summary for gGT1    The enzyme encoded by this gene is a type I gamma-glutamyltransferase that catalyzes the transfer of the glutamyl moiety of glutathione to a variety of amino acids and dipeptide acceptors. The enzyme is composed of a heavy chain and a light chain, which are derived from a single precursor protein. It is expressed in tissues involved in absorption and secretion and may contribute to the etiology of diabetes and other metabolic disorders. Multiple alternatively spliced variants have been identified. There are a number of related genes present on chromosomes 20 and 22, and putative pseudogenes for this gene on chromosomes 2, 13, and 22. [provided by RefSeq, Jan 2014]
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 UniProt Comments for gGT1    GGT1: Initiates extracellular glutathione (GSH) breakdown, provides cells with a local cysteine supply and contributes to maintain intracellular GSH level. It is part of the cell antioxidant defense mechanism. Catalyzes the transfer of the glutamyl moiety of glutathione to amino acids and dipeptide acceptors. Alternatively, glutathione can be hydrolyzed to give Cys-Gly and gamma glutamate. Isoform 3 seems to be inactive. Defects in GGT1 are a cause of glutathionuria (GLUTH); also known as gamma-glutamyltranspeptidase deficiency. It is an autosomal recessive disease. Belongs to the gamma-glutamyltransferase family. 3 isoforms of the human protein are produced by alternative promoter.

Protein type: EC 3.4.19.13; Other Amino Acids Metabolism - taurine and hypotaurine; Other Amino Acids Metabolism - selenoamino acid; EC 2.3.2.2; Transferase; EC 3.4.19.14; Other Amino Acids Metabolism - cyanoamino acid; Lipid Metabolism - arachidonic acid; Other Amino Acids Metabolism - glutathione; Membrane protein, integral

Chromosomal Location of Human Ortholog: 22q11.23

Cellular Component: anchored to external side of plasma membrane; extracellular space; plasma membrane; integral to membrane

Molecular Function: protein binding; gamma-glutamyltransferase activity

Biological Process: amino acid metabolic process; glutamate metabolic process; regulation of immune system process; cysteine biosynthetic process; leukotriene metabolic process; proteolysis; leukotriene biosynthetic process; glutathione metabolic process; xenobiotic metabolic process; regulation of inflammatory response; glutathione catabolic process; glutathione biosynthetic process; arachidonic acid metabolic process; spermatogenesis; zymogen activation

Disease: Glutathionuria
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 Research Articles on gGT1    1. The epidemiological association of alcohol intake with GGT is partly explained by genetic pleiotropy in this healthy population sample.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with gGT1 elisa kitPathways associated with gGT1 elisa kit
 Reference Product  PubMed Publications
 GPX1 elisa kit  >1 publications with gGT1 and GPX1
 GSTA1 elisa kit  >1 publications with gGT1 and GSTA1
 Products by Pathway  Pathway Diagram
 Aflatoxin Activation And Detoxification Pathway antibodies  Aflatoxin Activation And Detoxification Pathway Diagram
 Arachidonic Acid Metabolism Pathway antibodies  Arachidonic Acid Metabolism Pathway Diagram
 Arachidonic Acid Metabolism Pathway antibodies  Arachidonic Acid Metabolism Pathway Diagram
 Arachidonic Acid Metabolism Pathway antibodies  Arachidonic Acid Metabolism Pathway Diagram
 Biological Oxidations Pathway antibodies  Biological Oxidations Pathway Diagram
 Cyanoamino Acid Metabolism Pathway antibodies  Cyanoamino Acid Metabolism Pathway Diagram
 Cyanoamino Acid Metabolism Pathway antibodies  Cyanoamino Acid Metabolism Pathway Diagram
 Eicosanoid Synthesis Pathway antibodies  Eicosanoid Synthesis Pathway Diagram
 Glutathione Conjugation Pathway antibodies  Glutathione Conjugation Pathway Diagram
 Glutathione Metabolism Pathway antibodies  Glutathione Metabolism Pathway Diagram
Diseases associated with gGT1 elisa kitOrgans/Tissues associated with gGT1 elisa kit
 Disease Name  Pubmed Publications
 Liver Diseases Antibodies  >8 publications with gGT1 and Liver Diseases
 Carcinoma Antibodies  >6 publications with gGT1 and Carcinoma
 Breast Neoplasms Antibodies  >4 publications with gGT1 and Breast Neoplasms
 Liver Neoplasms Antibodies  >3 publications with gGT1 and Liver Neoplasms
 Heart Diseases Antibodies  >2 publications with gGT1 and Heart Diseases
 Lung Neoplasms Antibodies  >1 publications with gGT1 and Lung Neoplasms
 Neoplasms, Experimental Antibodies  >1 publications with gGT1 and Neoplasms, Experimental
 Carcinoma, Hepatocellular Antibodies  >1 publications with gGT1 and Carcinoma, Hepatocellular
 Inflammation Antibodies  >1 publications with gGT1 and Inflammation
 Liver Neoplasms, Experimental Antibodies  >1 publications with gGT1 and Liver Neoplasms, Experimental
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >8 publications with gGT1 and Blood
 Lung Antibodies  >6 publications with gGT1 and Lung
 Kidney Antibodies  >2 publications with gGT1 and Kidney
 Connective Tissue Antibodies  >1 publications with gGT1 and Connective Tissue
 Eye Antibodies  >1 publications with gGT1 and Eye
 Skin Antibodies  >1 publications with gGT1 and Skin
 Uncharacterized Tissue Antibodies  >1 publications with gGT1 and Uncharacterized Tissue
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