|
| Product Name
|
|
Ghrelin, ELISA Kit
|
| Also Known As
|
|
Canine Ghrelin ELISA Kit
|
| Product Gene Name
|
|
|
[Similar Products]
|
| Research Use Only
|
|
For Research Use Only. Not for use in diagnostic procedures.
|
 TOP
|
| Manual Insert
|
|
 
|
|
| Species Reactivity
|
|
Canine
|
|
| Specificity
|
|
No significant cross-reactivity or interference between this analyte and analogues is observed.
|
|
|
| Samples
|
|
Undiluted original Canine body fluids, tissue homogenates, secretions or feces samples.
|
| Assay Type
|
|
Sandwich (Quantitative)
|
| Detection Range
|
|
0.5 ng/ml - 16 ng/ml.
|
| Sensitivity
|
|
0.1 ng/ml.
|
| Intra-assay Precision
|
|
Intra-assay CV (%) is less than 15%.
|
| Inter-assay Precision
|
|
Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100].
|
|
|
| Preparation and Storage
|
|
Store all reagents at 2-8 degree C
|
| Product Note
|
|
Our ELISA assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay.
|
| Other Notes
|
|
Small volumes of GHRELIN elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
|
| Searchable Terms for GHRELIN purchase
|
|
MBS020808 is a ready-to-use microwell, strip plate Sandwich (Quantitative) ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Ghrelin, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 0.5 ng/ml - 16 ng/ml in biological research samples containing GHRELIN, with an estimated sensitivity of 0.1 ng/ml. The ELISA analytical biochemical technique of the MBS020808 kit is based on GHRELIN antibody-GHRELIN antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect GHRELIN antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, GHRELIN. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Undiluted original Canine body fluids, tissue homogenates, secretions or feces samples. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
|
|
TOP
|
|
Product Description specifically for GHRELIN elisa kit
|
|
Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! This kit is intended to be used for determination the level of GHRL (hereafter termed "analyte") in undiluted original Canine body fluids, tissue homogenates, secretions or feces samples. This kit is NOT suitable for assaying non-biological sources of substances.
|
|
| Sample Manual Insert of MBS020808 |
|
|
|
TOP
|
|
| NCBI/Uniprot data below describe general gene information for GHRELIN. It may not necessarily be applicable to this product. |
| NCBI GI #
|
|
304435649
|
| NCBI Accession #
|
|
ADM33790.1
|
[Other Products]
|
|
TOP
|
| NCBI Official Full Name
|
|
ghrelin
|
| Protein Family
|
|
Ghrelin
|
|
|
| NCBI Summary for GHRELIN
|
|
This gene encodes the ghrelin-obestatin preproprotein that is cleaved to yield two peptides, ghrelin and obestatin. Ghrelin is a powerful appetite stimulant and plays an important role in energy homeostasis. Its secretion is initiated when the stomach is empty, whereupon it binds to the growth hormone secretagogue receptor in the hypothalamus which results in the secretion of growth hormone (somatotropin). Ghrelin is thought to regulate multiple activities, including hunger, reward perception via the mesolimbic pathway, gastric acid secretion, gastrointestinal motility, and pancreatic glucose-stimulated insulin secretion. It was initially proposed that obestatin plays an opposing role to ghrelin by promoting satiety and thus decreasing food intake, but this action is still debated. Recent reports suggest multiple metabolic roles for obestatin, including regulating adipocyte function and glucose metabolism. Alternative splicing results in multiple transcript variants. In addition, antisense transcripts for this gene have been identified and may potentially regulate ghrelin-obestatin preproprotein expression. [provided by RefSeq, Nov 2014]
|
|
TOP
|
| Precautions
|
|
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
|
| Disclaimer
|
|
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
|
|
TOP
|
ELISA Kit 
