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GPBB elisa kit :: Canine Glycogen Phosphorylase lsoenzyme BB ELISA Kit

Scan QR to view Datasheet Catalog #    MBS735391
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $440 +1 FREE 8GB USB
96-Strip-Wells  /  $640 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,895 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,415 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Glycogen Phosphorylase lsoenzyme BB (GPBB), ELISA Kit

★Popular Item★
 Also Known As   

Canine Glycogen Phosphorylase lsoenzyme BB ELISA Kit

 Product Gene Name   

GPBB elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    138550
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 Species Reactivity    Canine
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 Samples    Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
 Assay Type    Sandwich
 Detection Range    2.5-50ng/mL
 Sensitivity    0.1ng/mL
 Intended Uses    This GPBB ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Canine GPBB. This ELISA kit for research use only, not for therapeutic or diagnostic applications
 !Intra-assay Precision    This assay has high sensitivity and excellent specificity for detection of GPBB. No significant cross-reactivity or interference between GPBB and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between GPBB and all the analogues, therefore, cross reaction may still exist in some cases.
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 Preparation and Storage    Store all reagents at 2-8 degree C.
 Sample Preparation    We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of GPBB elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for GPBB purchase    MBS735391 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Glycogen Phosphorylase lsoenzyme BB (GPBB) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 2.5-50ng/mL in biological research samples containing GPBB, with an estimated sensitivity of 0.1ng/mL. The ELISA analytical biochemical technique of the MBS735391 kit is based on GPBB antibody-GPBB antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect GPBB antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, GPBB. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for GPBB elisa kit

   Principle of the assay: GPBB ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for GPBB. Standards or samples are then added to the microtiter plate wells and GPBB if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of GPBB present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for GPBB are added to each well to “sandwich” the GPBB immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain GPBB and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The GPBB concentration in each sample is interpolated from this standard curve.
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 Typical Testing Data/Standard Curve (for reference only) of GPBB elisa kit    GPBB elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS735391. Click to request current manual
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NCBI/Uniprot data below describe general gene information for GPBB. It may not necessarily be applicable to this product.
 NCBI GI #    21361370
 NCBI GeneID    5834
 NCBI Accession #    NP_002853.2 [Other Products]
 NCBI GenBank Nucleotide #    NM_002862.3 [Other Products]
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 UniProt Secondary Accession #    Q96AK1; Q9NPX8 [Other Products]
 UniProt Related Accession #    P11216 [Other Products]
 Molecular Weight    96,696 Da
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 NCBI Official Full Name    glycogen phosphorylase, brain form
 NCBI Official Synonym Full Names    phosphorylase, glycogen; brain
 NCBI Official Symbol    PYGB [Similar Products]
 NCBI Official Synonym Symbols   
GPBB
[Similar Products]
 NCBI Protein Information    glycogen phosphorylase, brain form; glycogen phosphorylase B
 UniProt Protein Name    Glycogen phosphorylase, brain form
 UniProt Gene Name    PYGB [Similar Products]
 UniProt Entry Name    PYGB_HUMAN
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 NCBI Summary for GPBB    The protein encoded by this gene is a glycogen phosphorylase found predominantly in the brain. The encoded protein forms homodimers which can associate into homotetramers, the enzymatically active form of glycogen phosphorylase. The activity of this enzyme is positively regulated by AMP and negatively regulated by ATP, ADP, and glucose-6-phosphate. This enzyme catalyzes the rate-determining step in glycogen degradation. [provided by RefSeq, Jul 2008]
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 UniProt Comments for GPBB    PYGB: Phosphorylase is an important allosteric enzyme in carbohydrate metabolism. Enzymes from different sources differ in their regulatory mechanisms and in their natural substrates. However, all known phosphorylases share catalytic and structural properties. Belongs to the glycogen phosphorylase family.

Protein type: EC 2.4.1.1; Kinase, other; Carbohydrate Metabolism - starch and sucrose; Phosphorylase; Transferase

Chromosomal Location of Human Ortholog: 20p11.21

Cellular Component: membrane; cytoplasm

Molecular Function: glycogen phosphorylase activity; pyridoxal phosphate binding

Biological Process: glycogen catabolic process; carbohydrate metabolic process; glucose metabolic process; pathogenesis
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 Research Articles on GPBB    1. GPBB is a valuable biological marker to predict the prognosis in patient with acute coronary syndrome.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with GPBB elisa kitPathways associated with GPBB elisa kit
 Reference Product  PubMed Publications
 PGM1 elisa kit  >1 publications with GPBB and PGM1
 PHKB elisa kit  >1 publications with GPBB and PHKB
 Products by Pathway  Pathway Diagram
 Disease Pathway antibodies  Disease Pathway Diagram
 Glucose Metabolism Pathway antibodies  Glucose Metabolism Pathway Diagram
 Glycogen Metabolism Pathway antibodies  Glycogen Metabolism Pathway Diagram
 Glycogen Breakdown (glycogenolysis) Pathway antibodies  Glycogen Breakdown (glycogenolysis) Pathway Diagram
 Glycogen Storage Diseases Pathway antibodies  Glycogen Storage Diseases Pathway Diagram
 Insulin Signaling Pathway antibodies  Insulin Signaling Pathway Diagram
 Insulin Signaling Pathway antibodies  Insulin Signaling Pathway Diagram
 Metabolic Pathways antibodies  Metabolic Pathways Diagram
 Metabolism Pathway antibodies  Metabolism Pathway Diagram
 Metabolism Of Carbohydrates Pathway antibodies  Metabolism Of Carbohydrates Pathway Diagram
Diseases associated with GPBB elisa kitOrgans/Tissues associated with GPBB elisa kit
 Disease Name  Pubmed Publications
 Inflammation Antibodies  >2 publications with GPBB and Inflammation
 Adenocarcinoma Antibodies  >1 publications with GPBB and Adenocarcinoma
 Brain Diseases Antibodies  >1 publications with GPBB and Brain Diseases
 Carcinoma, Hepatocellular Antibodies  >1 publications with GPBB and Carcinoma, Hepatocellular
 Liver Diseases Antibodies  >1 publications with GPBB and Liver Diseases
 Disease Models, Animal Antibodies  >1 publications with GPBB and Disease Models, Animal
 Breast Neoplasms Antibodies  >1 publications with GPBB and Breast Neoplasms
 Nervous System Diseases Antibodies  >1 publications with GPBB and Nervous System Diseases
 Kidney Diseases Antibodies  >1 publications with GPBB and Kidney Diseases
 Liver Neoplasms Antibodies  >1 publications with GPBB and Liver Neoplasms
 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >5 publications with GPBB and Brain
 Liver Antibodies  >2 publications with GPBB and Liver
 Blood Antibodies  >2 publications with GPBB and Blood
 Muscle Antibodies  >2 publications with GPBB and Muscle
 Vascular Antibodies  >1 publications with GPBB and Vascular
 Heart Antibodies  >1 publications with GPBB and Heart
 Kidney Antibodies  >1 publications with GPBB and Kidney
 Pancreas Antibodies  >1 publications with GPBB and Pancreas
 Skin Antibodies  >1 publications with GPBB and Skin
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