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GYPA elisa kit :: Canine Glycophorin A ELISA Kit

Scan QR to view Datasheet Catalog #    MBS756356
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $470 +1 FREE 8GB USB
96-Strip-Wells  /  $675 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,925 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,490 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Glycophorin A (GYPA), ELISA Kit

 Also Known As   

Canine Glycophorin A ELISA Kit

 Product Gene Name   

GYPA elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Manual Insert    Download PDF Manual View PDF Manual
 OMIM    gene+611162
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 Species Reactivity    Canine
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 Samples    Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
 Assay Type    Competitive
 Sensitivity    1.0 pg/mL.
 Intended Uses    This GPA ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Canine GPA. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
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 Preparation and Storage    Store all reagents at 2-8 degree C.
 Sample Preparation    We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
 Product Note    Our ELISA assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay.
 Other Notes    Small volumes of GYPA elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for GYPA purchase    MBS756356 is a ready-to-use microwell, strip plate Competitive ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Glycophorin A (GYPA) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing GYPA, with an estimated sensitivity of 1.0 pg/mL. The ELISA analytical biochemical technique of the MBS756356 kit is based on GYPA antibody-GYPA antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect GYPA antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, GYPA. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%). RBCs are a conundrum for an organism - too "sticky" and clots develop without injury. Glycoproteins on the cell membrane create a hydrophilic coat preventing clumping. Glycophorin GYPA, is a known antigen, determining human blood group MNS types and a glycoprotein family group member. Detection of this critical protein in homo sapiens or in canine models is possible using sensitive antibody based products, like Cloud Clone Corp glycophorin ELISA. These ELISA kits excel in the assay of alpha 2 from beta 1 forms with high precision and accuracy. Utilizing the most sophisticated techniques, this competitive format group GYPA ELISA kit will provide reliable results from your valuable samples, The antibodies in all MyBiosource assay kit product catalogue items are top quality and validated to show a wide dynamic range typified by glycophorin MNS blood group GYPA kit 1. Use our services to order kit glycophorin and datasheet today.
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Product Description specifically for GYPA elisa kit

   Principle of the Assay: GPA ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-GPA antibody and an GPA-HRP conjugate. The assay sample and buffer are incubated together with GPA-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the GPA concentration since GPA from samples and GPA-HRP conjugate compete for the anti-GPA antibody binding site. Since the number of sites is limited, as more sites are occupied by GPA from the sample, fewer sites are left to bind GPA-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The GPA concentration in each sample is interpolated from this standard curve.
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 Typical Testing Data/Standard Curve (for reference only) of GYPA elisa kit    GYPA elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS756356
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NCBI/Uniprot data below describe general gene information for GYPA. It may not necessarily be applicable to this product.
 NCBI GI #    298286506
 NCBI GeneID    2993
 NCBI Accession #    NP_002090.4 [Other Products]
 NCBI GenBank Nucleotide #    NM_002099.6 [Other Products]
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 UniProt Secondary Accession #    Q9BS51; A8K3E6; B8Q182; B8Q185 [Other Products]
 UniProt Related Accession #    P02724 [Other Products]
 Molecular Weight    13,000 Da
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 NCBI Official Full Name    glycophorin-A
 NCBI Official Synonym Full Names    glycophorin A (MNS blood group)
 NCBI Official Symbol    GYPA [Similar Products]
 NCBI Official Synonym Symbols   
MN; GPA; MNS; GPSAT; PAS-2; CD235a; GPErik; HGpMiV; HGpMiXI; HGpSta(C)
[Similar Products]
 NCBI Protein Information    glycophorin-A; glycophorin MiI; glycophorin MiV; glycophorin SAT; glycophorin Erik; glycophorin A, GPA; MN sialoglycoprotein; glycophorin Sta type C; sialoglycoprotein alpha; Mi.V glycoprotein (24 AA); glycophorin A (MN blood group); recombinant glycophorin A-B Miltenberger-DR; erythroid-lineage-specific membrane sialoglycoprotein
 UniProt Protein Name    Glycophorin-A
 UniProt Synonym Protein Names   
MN sialoglycoprotein; PAS-2; Sialoglycoprotein alpha; CD_antigen: CD235a
 Protein Family    Glycophorin
 UniProt Gene Name    GYPA [Similar Products]
 UniProt Synonym Gene Names    GPA [Similar Products]
 UniProt Entry Name    GLPA_HUMAN
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 NCBI Summary for GYPA    Glycophorins A (GYPA) and B (GYPB) are major sialoglycoproteins of the human erythrocyte membrane which bear the antigenic determinants for the MN and Ss blood groups. In addition to the M or N and S or s antigens that commonly occur in all populations, about 40 related variant phenotypes have been identified. These variants include all the variants of the Miltenberger complex and several isoforms of Sta, as well as Dantu, Sat, He, Mg, and deletion variants Ena, S-s-U- and Mk. Most of the variants are the result of gene recombinations between GYPA and GYPB. [provided by RefSeq, Jul 2008]
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 UniProt Comments for GYPA    GYPA: Glycophorin A is the major intrinsic membrane protein of the erythrocyte. The N-terminal glycosylated segment, which lies outside the erythrocyte membrane, has MN blood group receptors. Appears to be important for the function of SLC4A1 and is required for high activity of SLC4A1. May be involved in translocation of SLC4A1 to the plasma membrane. Is a receptor for influenza virus. Is a receptor for Plasmodium falciparum erythrocyte-binding antigen 175 (EBA-175); binding of EBA-175 is dependent on sialic acid residues of the O-linked glycans. Appears to be a receptor for Hepatitis A virus (HAV). Belongs to the glycophorin A family.

Protein type: Adaptor/scaffold; Membrane protein, integral

Chromosomal Location of Human Ortholog: 4q31.21

Cellular Component: membrane; integral to plasma membrane; plasma membrane; external side of plasma membrane

Molecular Function: viral receptor activity; identical protein binding; protein homodimerization activity

Biological Process: entry of virus into host cell; cytoskeletal anchoring; regulation of response to osmotic stress

Disease: Malaria, Susceptibility To
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 Research Articles on GYPA    1. The expression of glycophorin A and osteoprotegerin is locally increased in carotid atherosclerotic lesions of symptomatic compared to asymptomatic patients.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with GYPA elisa kitPathways associated with GYPA elisa kit
 Reference Product  PubMed Publications
 GYPC elisa kit  >3 publications with GYPA and GYPC
 RHD elisa kit  >3 publications with GYPA and RHD
 SLC4A1 elisa kit  >2 publications with GYPA and SLC4A1
 Products by Pathway  Pathway Diagram
 Hematopoietic Cell Lineage Pathway antibodies  Hematopoietic Cell Lineage Pathway Diagram
 Hematopoietic Cell Lineage Pathway antibodies  Hematopoietic Cell Lineage Pathway Diagram
 Malaria Pathway antibodies  Malaria Pathway Diagram
 Malaria Pathway antibodies  Malaria Pathway Diagram
Diseases associated with GYPA elisa kitOrgans/Tissues associated with GYPA elisa kit
 Disease Name  Pubmed Publications
 Anemia Antibodies  >7 publications with GYPA and Anemia
 Malaria Antibodies  >6 publications with GYPA and Malaria
 Inflammation Antibodies  >4 publications with GYPA and Inflammation
 Necrosis Antibodies  >4 publications with GYPA and Necrosis
 Vascular Diseases Antibodies  >3 publications with GYPA and Vascular Diseases
 Cardiovascular Diseases Antibodies  >3 publications with GYPA and Cardiovascular Diseases
 Immune System Diseases Antibodies  >2 publications with GYPA and Immune System Diseases
 Liver Diseases Antibodies  >2 publications with GYPA and Liver Diseases
 Atherosclerosis Antibodies  >2 publications with GYPA and Atherosclerosis
 Respiratory Tract Diseases Antibodies  >2 publications with GYPA and Respiratory Tract Diseases
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >70 publications with GYPA and Blood
 Bone Marrow Antibodies  >6 publications with GYPA and Bone Marrow
 Embryonic Tissue Antibodies  >2 publications with GYPA and Embryonic Tissue
 Kidney Antibodies  >2 publications with GYPA and Kidney
 Liver Antibodies  >2 publications with GYPA and Liver
 Spleen Antibodies  >1 publications with GYPA and Spleen
 Placenta Antibodies  >1 publications with GYPA and Placenta
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