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IFN-alpha elisa kit :: Canine IFN-alpha ELISA Kit

Scan QR to view Datasheet Catalog #    MBS2516191 IFN-alpha elisa kit
Unit / Price
48-Strip-Wells  /  $490 +1 FREE 8GB USB
96-Strip-Wells  /  $585 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,475 +3 FREE 8GB USB
10x96-Strip-Wells  /  $4,360 +5 FREE 8GB USB
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

IFN-alpha, ELISA Kit

★Popular Item★
 Also Known As   

Canine IFN-alpha (Interferon Alpha) ELISA Kit

 Product Gene Name   

IFN-alpha elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS2516191 COA    COA PDF
 OMIM    147578
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 Species Reactivity    Canine
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 Specificity    This kit recognizes natural and some recombinant Canine IFN -?. No Significant cross -reactivity or interference between Canine IFN-? and analogues was observed.
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 Samples    Serum, plasma and other biological fluids
 Assay Type    Quantitative Sandwich
 Detection Range    31.25-2000 pg/mL
 Sensitivity    18.75pg/mL.
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 Preparation and Storage    Store at 4 degree C.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of IFN-alpha elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for IFN-alpha purchase    MBS2516191 is a ready-to-use microwell, strip plate Quantitative Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the IFN-alpha, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 31.25-2000 pg/mL in biological research samples containing IFN-alpha, with an estimated sensitivity of 18.75pg/mL. The ELISA analytical biochemical technique of the MBS2516191 kit is based on IFN-alpha antibody-IFN-alpha antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect IFN-alpha antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, IFN-alpha. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma and other biological fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for IFN-alpha elisa kit

   Intended Uses: This ELISA kit applies to the invitro quantitative determination of CanineIFN-alphaconcentrations in serum, plasma and other biological fluids.

Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to IFN-alpha. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for IFN-alphaand Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain IFN-alpha, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of IFN-alpha.You can calculate the concentration of IFN-alphain the samples by comparing the OD of the samples to the standard curve.
Sample Manual Insert of MBS2516191. Click to request current manual
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NCBI/Uniprot data below describe general gene information for IFN-alpha. It may not necessarily be applicable to this product.
 NCBI GI #    13128950
 NCBI GeneID    3439
 NCBI Accession #    NP_076918.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_024013.2 [Other Products]
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 UniProt Secondary Accession #    Q14605; Q2M1L8; Q52LB8; Q5VYQ2; Q7M4Q1; Q8WZ68; Q9UMJ3; D4Q9M8 [Other Products]
 UniProt Related Accession #    P01562 [Other Products]
 Molecular Weight    21,725 Da
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 NCBI Official Full Name    interferon alpha-1/13
 NCBI Official Synonym Full Names    interferon, alpha 1
 NCBI Official Symbol    IFNA1 [Similar Products]
 NCBI Official Synonym Symbols   
[Similar Products]
 NCBI Protein Information    interferon alpha-1/13; IFN-alpha 1b; IFN-alpha-1/13; interferon alpha 1b; interferon alpha-D; interferon-alpha1; leIF D
 UniProt Protein Name    Interferon alpha-1/13
 UniProt Synonym Protein Names   
Interferon alpha-D; LeIF D
 UniProt Gene Name    IFNA1 [Similar Products]
 UniProt Synonym Gene Names    IFN-alpha-1/13; LeIF D [Similar Products]
 UniProt Entry Name    IFNA1_HUMAN
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 NCBI Summary for IFN-alpha    The protein encoded by this gene is produced by macrophages and has antiviral activity. This gene is intronless and the encoded protein is secreted. [provided by RefSeq, Sep 2011]
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 UniProt Comments for IFN-alpha    IFNA1/13: a cytokine that belongs to the alpha/beta interferon family.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 9p22

Cellular Component: extracellular space; extracellular region

Molecular Function: interferon-alpha/beta receptor binding; cytokine activity

Biological Process: B cell proliferation; adaptive immune response; response to exogenous dsRNA; B cell differentiation; cytokine and chemokine mediated signaling pathway; natural killer cell activation during immune response; regulation of MHC class I biosynthetic process; innate immune response; blood coagulation; defense response to virus; humoral immune response; T cell activation during immune response; positive regulation of peptidyl-serine phosphorylation of STAT protein
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 Research Articles on IFN-alpha    1. The steady-state production of IFN-alpha plays a role in amplification of antiviral responses to control the spread of RNA viral infection in human hepatocytes.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with IFN-alpha elisa kitPathways associated with IFN-alpha elisa kit
 Reference Product  PubMed Publications
 IRF1 elisa kit  >3 publications with IFN-alpha and IRF1
 MX1 elisa kit  >3 publications with IFN-alpha and MX1
 STAT1 elisa kit  >2 publications with IFN-alpha and STAT1
 Products by Pathway  Pathway Diagram
 Autoimmune Thyroid Disease Pathway antibodies  Autoimmune Thyroid Disease Pathway Diagram
 Autoimmune Thyroid Disease Pathway antibodies  Autoimmune Thyroid Disease Pathway Diagram
 Cytokine Signaling In Immune System Pathway antibodies  Cytokine Signaling In Immune System Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 Cytosolic DNA-sensing Pathway antibodies  Cytosolic DNA-sensing Pathway Diagram
 Cytosolic DNA-sensing Pathway antibodies  Cytosolic DNA-sensing Pathway Diagram
 Factors Involved In Megakaryocyte Development And Platelet Production Pathway antibodies  Factors Involved In Megakaryocyte Development And Platelet Production Pathway Diagram
 Hemostasis Pathway antibodies  Hemostasis Pathway Diagram
 Hepatitis B Pathway antibodies  Hepatitis B Pathway Diagram
Diseases associated with IFN-alpha elisa kit
 Disease Name  Pubmed Publications
 Necrosis Antibodies  >8 publications with IFN-alpha and Necrosis
 Immune System Diseases Antibodies  >6 publications with IFN-alpha and Immune System Diseases
 Kidney Diseases Antibodies  >4 publications with IFN-alpha and Kidney Diseases
 Liver Diseases Antibodies  >3 publications with IFN-alpha and Liver Diseases
 Inflammation Antibodies  >3 publications with IFN-alpha and Inflammation
 Hepatitis B Antibodies  >2 publications with IFN-alpha and Hepatitis B
 Adenocarcinoma Antibodies  >2 publications with IFN-alpha and Adenocarcinoma
 Cardiovascular Diseases Antibodies  >2 publications with IFN-alpha and Cardiovascular Diseases
 Lung Neoplasms Antibodies  >2 publications with IFN-alpha and Lung Neoplasms
 Liver Neoplasms Antibodies  >2 publications with IFN-alpha and Liver Neoplasms
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