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IL-4 elisa kit :: Canine IL-4 ELISA Kit

Scan QR to view Datasheet Catalog #    MBS2516257 IL-4 elisa kit
Unit / Price
48-Strip-Wells  /  $490 +1 FREE 8GB USB
96-Strip-Wells  /  $585 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,475 +3 FREE 8GB USB
10x96-Strip-Wells  /  $4,360 +5 FREE 8GB USB
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 Product Name   


★Popular Item★
 Also Known As   

Canine IL-4 (Interleukin 4) ELISA Kit

 Product Gene Name   

IL-4 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS2516257 COA    COA PDF
 OMIM    147780
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 Species Reactivity    Canine
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 Specificity    This kit recognizes natural and some recombinant Canine IL-4. No Significant cross -reactivity or interference between Canine IL-4and analogues was observed.
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 Samples    Serum, plasma and other biological fluids
 Assay Type    Quantitative Sandwich
 Detection Range    15.63-1000 pg/mL
 Sensitivity    9.38pg/mL.
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 Preparation and Storage    Store at 4 degree C.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of IL-4 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for IL-4 purchase    MBS2516257 is a ready-to-use microwell, strip plate Quantitative Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the IL-4, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 15.63-1000 pg/mL in biological research samples containing IL-4, with an estimated sensitivity of 9.38pg/mL. The ELISA analytical biochemical technique of the MBS2516257 kit is based on IL-4 antibody-IL-4 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect IL-4 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, IL-4. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma and other biological fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for IL-4 elisa kit

   Intended Uses: This ELISA kit applies to the invitro quantitative determination of CanineIL-4concentrations in serum, plasma and other biological fluids.

Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to IL-4. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for IL-4and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain IL-4, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of IL-4.You can calculate the concentration of IL-4in the samples by comparing the OD of the samples to the standard curve.
Sample Manual Insert of MBS2516257. Click to request current manual
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NCBI/Uniprot data below describe general gene information for IL-4. It may not necessarily be applicable to this product.
 NCBI GI #    124337
 NCBI GeneID    3565
 NCBI Accession #    P05112.1 [Other Products]
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 UniProt Secondary Accession #    Q14630; Q6NZ77 [Other Products]
 UniProt Related Accession #    P05112 [Other Products]
 Molecular Weight    15,797 Da
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 NCBI Official Full Name    Interleukin-4
 NCBI Official Synonym Full Names    interleukin 4
 NCBI Official Symbol    IL4 [Similar Products]
 NCBI Official Synonym Symbols   
BSF1; IL-4; BCGF1; BSF-1; BCGF-1
[Similar Products]
 NCBI Protein Information    interleukin-4; B cell growth factor 1; B_cell stimulatory factor 1; binetrakin; interleukin 4 variant 2; lymphocyte stimulatory factor 1; pitrakinra
 UniProt Protein Name    Interleukin-4
 UniProt Synonym Protein Names   
B-cell stimulatory factor 1; BSF-1; Binetrakin; Lymphocyte stimulatory factor 1; Pitrakinra
 UniProt Gene Name    IL4 [Similar Products]
 UniProt Synonym Gene Names    IL-4; BSF-1 [Similar Products]
 UniProt Entry Name    IL4_HUMAN
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 NCBI Summary for IL-4    The protein encoded by this gene is a pleiotropic cytokine produced by activated T cells. This cytokine is a ligand for interleukin 4 receptor. The interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. STAT6, a signal transducer and activator of transcription, has been shown to play a central role in mediating the immune regulatory signal of this cytokine. This gene, IL3, IL5, IL13, and CSF2 form a cytokine gene cluster on chromosome 5q, with this gene particularly close to IL13. This gene, IL13 and IL5 are found to be regulated coordinately by several long-range regulatory elements in an over 120 kilobase range on the chromosome. Two alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008]
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 UniProt Comments for IL-4    IL4: Participates in at least several B-cell activation processes as well as of other cell types. It is a costimulator of DNA-synthesis. It induces the expression of class II MHC molecules on resting B-cells. It enhances both secretion and cell surface expression of IgE and IgG1. It also regulates the expression of the low affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes. Genetic variations in IL4 may be a cause of susceptibility to ischemic stroke (ISCHSTR); also known as cerebrovascular accident or cerebral infarction. A stroke is an acute neurologic event leading to death of neural tissue of the brain and resulting in loss of motor, sensory and/or cognitive function. Ischemic strokes, resulting from vascular occlusion, is considered to be a highly complex disease consisting of a group of heterogeneous disorders with multiple genetic and environmental risk factors. Belongs to the IL-4/IL-13 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cytokine; Secreted, signal peptide; Motility/polarity/chemotaxis; Secreted; Cell cycle regulation

Chromosomal Location of Human Ortholog: 5q31.1

Cellular Component: extracellular space; external side of plasma membrane

Molecular Function: protein binding; growth factor activity; cytokine activity; interleukin-4 receptor binding

Biological Process: regulation of isotype switching; positive regulation of isotype switching to IgG isotypes; negative regulation of osteoclast differentiation; positive regulation of transcription, DNA-dependent; regulation of proton transport; microglial cell activation; female pregnancy; positive regulation of activated T cell proliferation; chemotaxis; response to organic cyclic substance; positive regulation of interleukin-10 production; positive regulation of isotype switching to IgE isotypes; B cell costimulation; positive regulation of MHC class II biosynthetic process; connective tissue growth factor biosynthetic process; positive regulation of B cell proliferation; positive regulation of T cell proliferation; positive regulation of interleukin-13 production; negative regulation of macrophage activation; response to nutrient; response to drug; cholesterol metabolic process; regulation of immune response; negative regulation of chronic inflammatory response; T-helper 2 type immune response; regulation of phosphorylation; B cell activation; negative regulation of nitric oxide biosynthetic process; negative regulation of acute inflammatory response; defense response to protozoan; T-helper 1 cell lineage commitment; response to ethanol; positive regulation of chemokine biosynthetic process; innate immune response in mucosa; positive regulation of T cell differentiation; positive regulation of tyrosine phosphorylation of Stat5 protein; retina development in camera-type eye; response to cytokine stimulus; B cell differentiation; cellular defense response; immune response; positive regulation of transcription factor activity; positive regulation of transcription from RNA polymerase II promoter; T-helper 2 cell differentiation; negative regulation of transcription, DNA-dependent; positive regulation of defense response to virus by host; negative regulation of apoptosis

Disease: Stroke, Ischemic
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 Research Articles on IL-4    1. The data indicate no association between the two single nucleotide polymorphisms of IL-4-590 and IL-8-251 with overall gastric cancer risk.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with IL-4 elisa kitPathways associated with IL-4 elisa kit
 Reference Product  PubMed Publications
 IL5 elisa kit  >216 publications with IL-4 and IL5
 STAT6 elisa kit  >109 publications with IL-4 and STAT6
 IL4R elisa kit  >72 publications with IL-4 and IL4R
 CSF2 elisa kit  >18 publications with IL-4 and CSF2
 IL13RA1 elisa kit  >16 publications with IL-4 and IL13RA1
 JAK2 elisa kit  >10 publications with IL-4 and JAK2
 FOS elisa kit  >10 publications with IL-4 and FOS
 IL2RG elisa kit  >3 publications with IL-4 and IL2RG
 Products by Pathway  Pathway Diagram
 Allograft Rejection Pathway antibodies  Allograft Rejection Pathway Diagram
 Allograft Rejection Pathway antibodies  Allograft Rejection Pathway Diagram
 Allograft Rejection Pathway antibodies  Allograft Rejection Pathway Diagram
 Asthma Pathway antibodies  Asthma Pathway Diagram
 Asthma Pathway antibodies  Asthma Pathway Diagram
 Autoimmune Thyroid Disease Pathway antibodies  Autoimmune Thyroid Disease Pathway Diagram
 Autoimmune Thyroid Disease Pathway antibodies  Autoimmune Thyroid Disease Pathway Diagram
 CD40/CD40L Signaling Pathway antibodies  CD40/CD40L Signaling Pathway Diagram
 Calcineurin-regulated NFAT-dependent Transcription In Lymphocytes Pathway antibodies  Calcineurin-regulated NFAT-dependent Transcription In Lymphocytes Pathway Diagram
 Calcium Signaling In The CD4+ TCR Pathway antibodies  Calcium Signaling In The CD4+ TCR Pathway Diagram
Diseases associated with IL-4 elisa kitOrgans/Tissues associated with IL-4 elisa kit
 Disease Name  Pubmed Publications
 Inflammation Antibodies  >349 publications with IL-4 and Inflammation
 Hypersensitivity Antibodies  >334 publications with IL-4 and Hypersensitivity
 Necrosis Antibodies  >330 publications with IL-4 and Necrosis
 Hypersensitivity, Immediate Antibodies  >270 publications with IL-4 and Hypersensitivity, Immediate
 Asthma Antibodies  >234 publications with IL-4 and Asthma
 Lung Diseases Antibodies  >215 publications with IL-4 and Lung Diseases
 Respiratory Hypersensitivity Antibodies  >201 publications with IL-4 and Respiratory Hypersensitivity
 Autoimmune Diseases Antibodies  >174 publications with IL-4 and Autoimmune Diseases
 Liver Diseases Antibodies  >58 publications with IL-4 and Liver Diseases
 Rhinitis Antibodies  >49 publications with IL-4 and Rhinitis
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >1312 publications with IL-4 and Blood
 Spleen Antibodies  >102 publications with IL-4 and Spleen
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