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ITIH2 elisa kit :: Canine Inter-alpha-trypsin inhibitor heavy chain H2 (ITIH2) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS7238813
Typical Testing Data/Standard Curve
Unit / Price
48-Strip-Wells  /  $440 +1 FREE 8GB USB
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 Product Name   

Inter-alpha-trypsin inhibitor heavy chain H2 (ITIH2), ELISA Kit

★Popular Item★
 Also Known As   

Canine Inter-alpha-trypsin inhibitor heavy chain H2 (ITIH2) ELISA Kit

 Product Gene Name   

ITIH2 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    146640
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 Species Reactivity    Canine
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 Specificity    This assay has high sensitivity and excellent specificity for detection of ITIH2. No significant cross-reactivity or interference between ITIH2 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between ITIH2 and all the analogues, therefore, cross reaction may still exist in some cases.
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 Samples    Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
 Assay Type    Competitive
 Sensitivity    0.1 ug/mL.
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 Preparation and Storage    Store all reagents at 2-8 degree C.
 Sample Preparation    We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of ITIH2 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for ITIH2 purchase    MBS7238813 is a ready-to-use microwell, strip plate Competitive ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Inter-alpha-trypsin inhibitor heavy chain H2 (ITIH2) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing ITIH2, with an estimated sensitivity of 0.1 ug/mL. The ELISA analytical biochemical technique of the MBS7238813 kit is based on ITIH2 antibody-ITIH2 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect ITIH2 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, ITIH2. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, cell culture supernatants, body fluid and tissue homogenate. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for ITIH2 elisa kit

   Intended Uses: This ITIH2 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Canine ITIH2. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Principle of the Assay||ITIH2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-ITIH2 antibody and an ITIH2-HRP conjugate. The assay sample and buffer are incubated together with ITIH2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ITIH2 concentration since ITIH2 from samples and ITIH2-HRP conjugate compete for the anti-ITIH2 antibody binding site. Since the number of sites is limited, as more sites are occupied by ITIH2 from the sample, fewer sites are left to bind ITIH2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ITIH2 concentration in each sample is interpolated from this standard curve.
 Product Categories/Family for ITIH2 elisa kit    Cell Biology
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 Typical Testing Data/Standard Curve of ITIH2 elisa kit    ITIH2 elisa kit Typical Testing Data/Standard Curve image
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Sample Manual Insert of MBS7238813. Click to request current manual
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NCBI/Uniprot data below describe general gene information for ITIH2. It may not necessarily be applicable to this product.
 NCBI GI #    70778918
 NCBI GeneID    3698
 NCBI Accession #    NP_002207.2 [Other Products]
 NCBI GenBank Nucleotide #    NM_002216.2 [Other Products]
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 UniProt Secondary Accession #    Q14659; Q15484; Q5T986 [Other Products]
 UniProt Related Accession #    P19823 [Other Products]
 Molecular Weight    106,463 Da
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 NCBI Official Full Name    inter-alpha-trypsin inhibitor heavy chain H2
 NCBI Official Synonym Full Names    inter-alpha-trypsin inhibitor heavy chain 2
 NCBI Official Symbol    ITIH2 [Similar Products]
 NCBI Official Synonym Symbols   
[Similar Products]
 NCBI Protein Information    inter-alpha-trypsin inhibitor heavy chain H2; ITI-HC2; ITI heavy chain H2; inter-alpha (globulin) inhibitor H2; inter-alpha-inhibitor heavy chain 2; serum-derived hyaluronan-associated protein; inter-alpha (globulin) inhibitor, H2 polypeptide; inter-alpha-trypsin inhibitor complex component II
 UniProt Protein Name    Inter-alpha-trypsin inhibitor heavy chain H2
 UniProt Synonym Protein Names   
Inter-alpha-trypsin inhibitor complex component II; Serum-derived hyaluronan-associated protein; SHAP
 Protein Family    Inter-alpha-trypsin inhibitor
 UniProt Gene Name    ITIH2 [Similar Products]
 UniProt Synonym Gene Names    IGHEP2; ITI heavy chain H2; ITI-HC2; Inter-alpha-inhibitor heavy chain 2; SHAP [Similar Products]
 UniProt Entry Name    ITIH2_HUMAN
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 NCBI Summary for ITIH2    The inter-alpha-trypsin inhibitors (ITI) are a family of structurally related plasma serine protease inhibitors involved in extracellular matrix stabilization and in prevention of tumor metastasis. The ITI family contains multiple proteins made up of a light chain (see MIM 176870) and a variable number of heavy chains (Salier et al., 1987 [PubMed 2446322]; Himmelfarb et al., 2004 [PubMed 14744536]).[supplied by OMIM, Nov 2009]
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 UniProt Comments for ITIH2    ITIH2: May act as a carrier of hyaluronan in serum or as a binding protein between hyaluronan and other matrix protein, including those on cell surfaces in tissues to regulate the localization, synthesis and degradation of hyaluronan which are essential to cells undergoing biological processes. Belongs to the ITIH family.

Protein type: Secreted; Inhibitor; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 10p15

Cellular Component: extracellular region

Molecular Function: serine-type endopeptidase inhibitor activity; endopeptidase inhibitor activity

Biological Process: hyaluronan metabolic process
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 Research Articles on ITIH2    1. Human inter-alpha-inhibitor is a substrate for factor XIIIa and tissue transglutaminase.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with ITIH2 elisa kitDiseases associated with ITIH2 elisa kit
 Reference Product  PubMed Publications
 APOH elisa kit  >1 publications with ITIH2 and APOH
 AMBP elisa kit  >1 publications with ITIH2 and AMBP
 Disease Name  Pubmed Publications
 Carcinoma Antibodies  >2 publications with ITIH2 and Carcinoma
 Lung Neoplasms Antibodies  >2 publications with ITIH2 and Lung Neoplasms
 Necrosis Antibodies  >2 publications with ITIH2 and Necrosis
 Breast Neoplasms Antibodies  >1 publications with ITIH2 and Breast Neoplasms
 Immune System Diseases Antibodies  >1 publications with ITIH2 and Immune System Diseases
 Inflammation Antibodies  >1 publications with ITIH2 and Inflammation
Organs/Tissues associated with ITIH2 elisa kit
 Organ/Tissue Name  Pubmed Publications
 Stomach Antibodies  >2 publications with ITIH2 and Stomach
 Pancreas Antibodies  >2 publications with ITIH2 and Pancreas
 Uterus Antibodies  >2 publications with ITIH2 and Uterus
 Kidney Antibodies  >1 publications with ITIH2 and Kidney
 Brain Antibodies  >1 publications with ITIH2 and Brain
 Liver Antibodies  >1 publications with ITIH2 and Liver
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