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IL-13 elisa kit :: Canine Interleukin 13 ELISA Kit

Scan QR to view Datasheet Catalog #    MBS029179 IL-13 elisa kit
Unit / Price
48-Strip-Wells  /  $435 +1 FREE 8GB USB
96-Strip-Wells  /  $600 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,650 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,205 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Interleukin 13 (IL-13), ELISA Kit

 Also Known As   

Canine Interleukin 13 ELISA Kit

 Product Gene Name   

IL-13 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Manual Insert    Download PDF Manual View PDF Manual
 Chromosome Location    Chromosome: 5; NC_000005.9 (131993865..131996801). Location: 5q31
 OMIM    147683
 3D Structure    ModBase 3D Structure for P35225
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 Species Reactivity    Canine
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 Specificity    No significant cross-reactivity or interference between Canine IL-13 and analogues was observed.
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 Samples    Serum, Plasma, Tissue Homogenate, Feces and Urine
 Assay Type    Sandwich
 Detection Range    6.25pg/ml-200pg/ml.
 Sensitivity    1.0pg/ml.
 Intended Uses    This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated IL-13 concentrations in Canine serum, plasma and other body fluids. Using Purified Canine IL-13 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add IL-13 and IL-13 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of IL-13 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
 Intra-assay Precision    Intra-assay CV (%) is less than 15%.
 Inter-assay Precision    Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean 100]
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 Preparation and Storage    Store all reagents at 2-8 degree C
 Product Note    Our ELISA assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay.
 Other Notes    Small volumes of IL-13 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for IL-13 purchase    MBS029179 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Interleukin 13 (IL-13) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 6.25pg/ml-200pg/ml in biological research samples containing IL-13, with an estimated sensitivity of 1.0pg/ml. The ELISA analytical biochemical technique of the MBS029179 kit is based on IL-13 antibody-IL-13 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect IL-13 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, IL-13. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, Plasma, Tissue Homogenate, Feces and Urine. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Product Description specifically for IL-13 elisa kit

   Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated IL-13 concentrations in Canine serum, plasma and other body fluids. Using Purified Canine IL-13 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add IL-13 and IL-13 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of IL-13 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Sample Manual Insert of MBS029179
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NCBI/Uniprot data below describe general gene information for IL-13. It may not necessarily be applicable to this product.
 NCBI GI #    26787978
 NCBI GeneID    3596
 NCBI Accession #    NP_002179.2 [Other Products]
 NCBI GenBank Nucleotide #    NM_002188.2 [Other Products]
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 UniProt Primary Accession #    P35225 [Other Products]
 UniProt Secondary Accession #    O43644; Q4VB52; Q9UDC7 [Other Products]
 UniProt Related Accession #    P35225 [Other Products]
 Molecular Weight    15,816 Da
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 NCBI Official Full Name    interleukin-13
 NCBI Official Synonym Full Names    interleukin 13
 NCBI Official Symbol    IL13 [Similar Products]
 NCBI Official Synonym Symbols   
P600; IL-13
[Similar Products]
 NCBI Protein Information    interleukin-13
 UniProt Protein Name    Interleukin-13
 UniProt Gene Name    IL13 [Similar Products]
 UniProt Synonym Gene Names    NC30; IL-13 [Similar Products]
 UniProt Entry Name    IL13_HUMAN
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 NCBI Summary for IL-13    This gene encodes an immunoregulatory cytokine produced primarily by activated Th2 cells. This cytokine is involved in several stages of B-cell maturation and differentiation. It up-regulates CD23 and MHC class II expression, and promotes IgE isotype switching of B cells. This cytokine down-regulates macrophage activity, thereby inhibits the production of pro-inflammatory cytokines and chemokines. This cytokine is found to be critical to the pathogenesis of allergen-induced asthma but operates through mechanisms independent of IgE and eosinophils. This gene, IL3, IL5, IL4, and CSF2 form a cytokine gene cluster on chromosome 5q, with this gene particularly close to IL4. [provided by RefSeq, Jul 2008]
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 UniProt Comments for IL-13    IL13: Cytokine. Inhibits inflammatory cytokine production. Synergizes with IL2 in regulating interferon-gamma synthesis. May be critical in regulating inflammatory and immune responses. Defects in IL13 may be a cause of susceptibility to allergic rhinitis (ALRH). Allergic rhinitis is a common disease of complex inheritance and is characterized by mucosal inflammation caused by allergen exposure. Belongs to the IL-4/IL-13 family.

Protein type: Secreted; Motility/polarity/chemotaxis; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 5q31

Cellular Component: extracellular space; cytoplasm; extracellular region; external side of plasma membrane

Molecular Function: protein binding; interleukin-13 receptor binding; cytokine activity

Biological Process: response to nicotine; positive regulation of smooth muscle cell proliferation; microglial cell activation; regulation of proton transport; response to lipopolysaccharide; signal transduction; positive regulation of connective tissue growth factor production; positive regulation of tyrosine phosphorylation of Stat6 protein; positive regulation of immunoglobulin production; response to ethanol; positive regulation of protein secretion; cell-cell signaling; positive regulation of B cell proliferation; immune response; positive regulation of release of sequestered calcium ion into cytosol; inflammatory response; negative regulation of NAD(P)H oxidase activity; cell motility; positive regulation of macrophage activation

Disease: Asthma, Susceptibility To; Allergic Rhinitis
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 Research Articles on IL-13    1. A meta-analysis indicates that IL-13 C-1112T and G+2044A polymorphisms could be proposed as asthma susceptible SNPs.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with IL-13 elisa kitPathways associated with IL-13 elisa kit
 Reference Product  PubMed Publications
 STAT6 elisa kit  >379 publications with IL-13 and STAT6
 IL13RA1 elisa kit  >287 publications with IL-13 and IL13RA1
 IL5 elisa kit  >86 publications with IL-13 and IL5
 IL18 elisa kit  >83 publications with IL-13 and IL18
 GATA3 elisa kit  >75 publications with IL-13 and GATA3
 IL4R elisa kit  >56 publications with IL-13 and IL4R
 TSLP elisa kit  >26 publications with IL-13 and TSLP
 IL13RA2 elisa kit  >11 publications with IL-13 and IL13RA2
 VCAM1 elisa kit  >5 publications with IL-13 and VCAM1
 Products by Pathway  Pathway Diagram
 Asthma Pathway antibodies  Asthma Pathway Diagram
 Asthma Pathway antibodies  Asthma Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 Cytokines And Inflammatory Response Pathway antibodies  Cytokines And Inflammatory Response Pathway Diagram
 Fc Epsilon RI Signaling Pathway antibodies  Fc Epsilon RI Signaling Pathway Diagram
 Fc Epsilon RI Signaling Pathway antibodies  Fc Epsilon RI Signaling Pathway Diagram
 Glucocorticoid Receptor Regulatory Network Pathway antibodies  Glucocorticoid Receptor Regulatory Network Pathway Diagram
 IL12 Signaling Mediated By STAT4 Pathway antibodies  IL12 Signaling Mediated By STAT4 Pathway Diagram
 Inflammatory Bowel Disease Pathway antibodies  Inflammatory Bowel Disease Pathway Diagram
Diseases associated with IL-13 elisa kitOrgans/Tissues associated with IL-13 elisa kit
 Disease Name  Pubmed Publications
 Hypersensitivity Antibodies  >1222 publications with IL-13 and Hypersensitivity
 Inflammation Antibodies  >1147 publications with IL-13 and Inflammation
 Asthma Antibodies  >1137 publications with IL-13 and Asthma
 Hypersensitivity, Immediate Antibodies  >1058 publications with IL-13 and Hypersensitivity, Immediate
 Lung Diseases Antibodies  >1041 publications with IL-13 and Lung Diseases
 Respiratory Hypersensitivity Antibodies  >882 publications with IL-13 and Respiratory Hypersensitivity
 Necrosis Antibodies  >562 publications with IL-13 and Necrosis
 Fibrosis Antibodies  >290 publications with IL-13 and Fibrosis
 Bronchial Hyperreactivity Antibodies  >211 publications with IL-13 and Bronchial Hyperreactivity
 Nervous System Diseases Antibodies  >176 publications with IL-13 and Nervous System Diseases
 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >160 publications with IL-13 and Brain
 Bone Marrow Antibodies  >126 publications with IL-13 and Bone Marrow
 Mixed Antibodies  >65 publications with IL-13 and Mixed
 Testis Antibodies  >6 publications with IL-13 and Testis
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