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IL-31 elisa kit :: Canine Interleukin 31 ELISA Kit

Scan QR to view Datasheet Catalog #    MBS740462
 Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $440 +1 FREE 8GB USB
96-Strip-Wells  /  $640 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,895 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,415 +6 FREE 8GB USB
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 Product Name   

Interleukin 31 (IL-31), ELISA Kit

★Popular Item★
 Also Known As   

Canine Interleukin 31 ELISA Kit

 Product Gene Name   

IL-31 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    609509
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 Species Reactivity    Canine
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 Specificity    This assay has high sensitivity and excellent specificity for detection of IL-31. No significant cross-reactivity or interference between IL-31 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between IL-31 and all the analogues, therefore, cross reaction may still exist in some cases.
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 Samples    Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
 Assay Type    Quantitative Competitive
 Sensitivity    1.0 pg/mL.
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 Preparation and Storage    Store all reagents at 2-8 degree C.
 Sample Preparation    We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of IL-31 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for IL-31 purchase    MBS740462 is a ready-to-use microwell, strip plate Quantitative Competitive ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Interleukin 31 (IL-31) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing IL-31, with an estimated sensitivity of 1.0 pg/mL. The ELISA analytical biochemical technique of the MBS740462 kit is based on IL-31 antibody-IL-31 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect IL-31 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, IL-31. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, cell culture supernatants, body fluid and tissue homogenate. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for IL-31 elisa kit

   Intended Uses: This IL-31 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Canine IL-31. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Principle of the Assay: IL-31 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-IL-31 antibody and an IL-31-HRP conjugate. The assay sample and buffer are incubated together with IL-31-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the IL-31 concentration since IL-31 from samples and IL-31-HRP conjugate compete for the anti-IL-31 antibody binding site. Since the number of sites is limited, as more sites are occupied by IL-31 from the sample, fewer sites are left to bind IL-31-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The IL-31 concentration in each sample is interpolated from this standard curve.
 Product Categories/Family for IL-31 elisa kit    Immunology
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  Typical Testing Data/Standard Curve (for reference only) of IL-31 elisa kit    IL-31 elisa kit  Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS740462. Click to request current manual
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NCBI/Uniprot data below describe general gene information for IL-31. It may not necessarily be applicable to this product.
 NCBI GI #    62122911
 NCBI GeneID    386653
 NCBI Accession #    NP_001014358.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001014336.1 [Other Products]
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 UniProt Secondary Accession #    A2RUQ1 [Other Products]
 UniProt Related Accession #    Q6EBC2 [Other Products]
 Molecular Weight    18,205 Da
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 NCBI Official Full Name    interleukin-31
 NCBI Official Synonym Full Names    interleukin 31
 NCBI Official Symbol    IL31 [Similar Products]
 NCBI Official Synonym Symbols   
[Similar Products]
 NCBI Protein Information    interleukin-31
 UniProt Protein Name    Interleukin-31
 UniProt Gene Name    IL31 [Similar Products]
 UniProt Synonym Gene Names    IL-31 [Similar Products]
 UniProt Entry Name    IL31_HUMAN
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 NCBI Summary for IL-31    IL31, which is made principally by activated Th2-type T cells, interacts with a heterodimeric receptor consisting of IL31RA (MIM 609510) and OSMR (MIM 601743) that is constitutively expressed on epithelial cells and keratinocytes. IL31 may be involved in the promotion of allergic skin disorders and in regulating other allergic diseases, such as asthma (Dillon et al., 2004 [PubMed 15184896]).[supplied by OMIM, Mar 2008]
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 UniProt Comments for IL-31    IL31: Activates STAT3 and possibly STAT1 and STAT5 through the IL31 heterodimeric receptor composed of IL31RA and OSMR. IL31 may function in skin immunity.

Protein type: Secreted, signal peptide; Secreted; Cytokine

Chromosomal Location of Human Ortholog: 12q24.31

Cellular Component: extracellular space

Molecular Function: cytokine activity

Biological Process: immune system process
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 Research Articles on IL-31    1. EGFR-TK inhibitors could cause keratinocytes injury, the release of IL-33 and the consequent interaction with its receptor on mast cells, that induces the secretion of several factors capable to cause skin manifestations, included IL-31
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with IL-31 elisa kitDiseases associated with IL-31 elisa kit
 Reference Product  PubMed Publications
 IL31RA elisa kit  >14 publications with IL-31 and IL31RA
 OSMR elisa kit  >8 publications with IL-31 and OSMR
 IL6ST elisa kit  >1 publications with IL-31 and IL6ST
 Disease Name  Pubmed Publications
 Dermatitis Antibodies  >46 publications with IL-31 and Dermatitis
 Dermatitis, Atopic Antibodies  >41 publications with IL-31 and Dermatitis, Atopic
 Hypersensitivity Antibodies  >40 publications with IL-31 and Hypersensitivity
 Inflammation Antibodies  >23 publications with IL-31 and Inflammation
 Lung Diseases Antibodies  >7 publications with IL-31 and Lung Diseases
 Respiratory Hypersensitivity Antibodies  >6 publications with IL-31 and Respiratory Hypersensitivity
 Dermatitis, Contact Antibodies  >5 publications with IL-31 and Dermatitis, Contact
 Necrosis Antibodies  >3 publications with IL-31 and Necrosis
 Brain Diseases Antibodies  >3 publications with IL-31 and Brain Diseases
 Melanoma Antibodies  >2 publications with IL-31 and Melanoma
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