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IL-6R elisa kit :: Canine Interleukin 6 receptor ELISA Kit

Scan QR to view Datasheet Catalog #    MBS744847
Typical Testing Data/Standard Curve
Unit / Price
48-Strip-Wells  /  $440 +1 FREE 8GB USB
96-Strip-Wells  /  $640 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,895 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,415 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Interleukin 6 receptor (IL-6R), ELISA Kit

★Popular Item★
 Also Known As   

Canine Interleukin 6 receptor ELISA Kit

 Product Gene Name   

IL-6R elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    614752
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 Species Reactivity    Canine
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 Specificity    This assay has high sensitivity and excellent specificity for detection of IL-6R. No significant cross-reactivity or interference between IL-6R and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between IL-6R and all the analogues, therefore, cross reaction may still exist in some cases.
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 Samples    Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
 Assay Type    Competitive
 Sensitivity    0.1 ng/mL.
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 Preparation and Storage    Store all reagents at 2-8 degree C.
 Sample Preparation    We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of IL-6R elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for IL-6R purchase    MBS744847 is a ready-to-use microwell, strip plate Competitive ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Interleukin 6 receptor (IL-6R) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing IL-6R, with an estimated sensitivity of 0.1 ng/mL. The ELISA analytical biochemical technique of the MBS744847 kit is based on IL-6R antibody-IL-6R antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect IL-6R antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, IL-6R. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, cell culture supernatants, body fluid and tissue homogenate. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for IL-6R elisa kit

   Intended Uses: This IL-6R ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Canine IL-6R. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Principle of the Assay||IL-6R ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-IL-6R antibody and an IL-6R-HRP conjugate. The assay sample and buffer are incubated together with IL-6R-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the IL-6R concentration since IL-6R from samples and IL-6R-HRP conjugate compete for the anti-IL-6R antibody binding site. Since the number of sites is limited, as more sites are occupied by IL-6R from the sample, fewer sites are left to bind IL-6R-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The IL-6R concentration in each sample is interpolated from this standard curve.
 Product Categories/Family for IL-6R elisa kit    Immunology
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 Typical Testing Data/Standard Curve of IL-6R elisa kit    IL-6R elisa kit Typical Testing Data/Standard Curve image
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Sample Manual Insert of MBS744847. Click to request current manual
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NCBI/Uniprot data below describe general gene information for IL-6R. It may not necessarily be applicable to this product.
 NCBI GI #    332309226
 NCBI GeneID    3570
 NCBI Accession #    NP_001193795.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001206866.1 [Other Products]
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 UniProt Secondary Accession #    Q16202; Q53EQ7; Q5FWG2; Q5VZ23; A8KAE8; B2R6V4 [Other Products]
 UniProt Related Accession #    P08887 [Other Products]
 Molecular Weight    40,237 Da
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 NCBI Official Full Name    interleukin-6 receptor subunit alpha isoform 3
 NCBI Official Synonym Full Names    interleukin 6 receptor
 NCBI Official Symbol    IL6R [Similar Products]
 NCBI Official Synonym Symbols   
IL6Q; gp80; CD126; IL6RA; IL6RQ; IL-6RA; IL-6R-1
[Similar Products]
 NCBI Protein Information    interleukin-6 receptor subunit alpha; IL-6R 1; CD126 antigen; membrane glycoprotein 80; IL-6 receptor subunit alpha
 UniProt Protein Name    Interleukin-6 receptor subunit alpha
 UniProt Synonym Protein Names   
IL-6R 1; Membrane glycoprotein 80; gp80; CD_antigen: CD126
 UniProt Gene Name    IL6R [Similar Products]
 UniProt Synonym Gene Names    IL-6 receptor subunit alpha; IL-6R subunit alpha; IL-6R-alpha; IL-6RA; gp80 [Similar Products]
 UniProt Entry Name    IL6RA_HUMAN
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 NCBI Summary for IL-6R    This gene encodes a subunit of the interleukin 6 (IL6) receptor complex. Interleukin 6 is a potent pleiotropic cytokine that regulates cell growth and differentiation and plays an important role in the immune response. The IL6 receptor is a protein complex consisting of this protein and interleukin 6 signal transducer (IL6ST/GP130/IL6-beta), a receptor subunit also shared by many other cytokines. Dysregulated production of IL6 and this receptor are implicated in the pathogenesis of many diseases, such as multiple myeloma, autoimmune diseases and prostate cancer. Alternatively spliced transcript variants encoding distinct isoforms have been reported. A pseudogene of this gene is found on chromosome 9.[provided by RefSeq, May 2011]
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 UniProt Comments for IL-6R    IL6R: Part of the receptor for interleukin 6. Binds to IL6 with low affinity, but does not transduce a signal. Signal activation necessitate an association with IL6ST. Activation may lead to the regulation of the immune response, acute-phase reactions and hematopoiesis. Belongs to the type I cytokine receptor family. Type 3 subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Receptor, cytokine

Chromosomal Location of Human Ortholog: 1q21

Cellular Component: extracellular space; basolateral plasma membrane; apical plasma membrane; extracellular region; plasma membrane; interleukin-6 receptor complex

Molecular Function: protein binding; protein homodimerization activity; interleukin-6 binding; interleukin-6 receptor binding; enzyme binding; ciliary neurotrophic factor receptor activity; interleukin-6 receptor activity

Biological Process: negative regulation of collagen biosynthetic process; cytokine and chemokine mediated signaling pathway; positive regulation of smooth muscle cell proliferation; neutrophil mediated immunity; positive regulation of interleukin-6 production; positive regulation of leukocyte chemotaxis; defense response to Gram-negative bacterium; endocrine pancreas development; positive regulation of chemokine production; positive regulation of tyrosine phosphorylation of Stat3 protein; activation of NF-kappaB transcription factor; monocyte chemotaxis; positive regulation of osteoblast differentiation; defense response to Gram-positive bacterium; positive regulation of peptidyl-tyrosine phosphorylation; positive regulation of MAPKKK cascade; response to cytokine stimulus; positive regulation of cell proliferation; acute-phase response; negative regulation of interleukin-8 production; hepatic immune response

Disease: Interleukin 6, Serum Level Of, Quantitative Trait Locus; Soluble Interleukin-6 Receptor, Serum Level Of, Quantitative Trait Locus
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 Research Articles on IL-6R    1. IL-6R antibody could significantly increase the expression of OPG, but inhibit the expression of RANKL, which might provide a theoretical basis of molecular biology for the prevention and treatment of aseptic loosening of prosthesis.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with IL-6R elisa kitPathways associated with IL-6R elisa kit
 Reference Product  PubMed Publications
 IL6 elisa kit  >108 publications with IL-6R and IL6
 STAT3 elisa kit  >29 publications with IL-6R and STAT3
 IL6ST elisa kit  >18 publications with IL-6R and IL6ST
 SOCS3 elisa kit  >3 publications with IL-6R and SOCS3
 STAT1 elisa kit  >3 publications with IL-6R and STAT1
 JAK2 elisa kit  >3 publications with IL-6R and JAK2
 JAK1 elisa kit  >3 publications with IL-6R and JAK1
 CNTF elisa kit  >1 publications with IL-6R and CNTF
 Products by Pathway  Pathway Diagram
 Cytokine Signaling In Immune System Pathway antibodies  Cytokine Signaling In Immune System Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 HIF-1 Signaling Pathway antibodies  HIF-1 Signaling Pathway Diagram
 Hematopoietic Cell Lineage Pathway antibodies  Hematopoietic Cell Lineage Pathway Diagram
 Hematopoietic Cell Lineage Pathway antibodies  Hematopoietic Cell Lineage Pathway Diagram
 IL-6 Signaling Pathway antibodies  IL-6 Signaling Pathway Diagram
 IL6-mediated Signaling Events Pathway antibodies  IL6-mediated Signaling Events Pathway Diagram
 Immune System Pathway antibodies  Immune System Pathway Diagram
 Interleukin-6 Signaling Pathway antibodies  Interleukin-6 Signaling Pathway Diagram
Diseases associated with IL-6R elisa kitOrgans/Tissues associated with IL-6R elisa kit
 Disease Name  Pubmed Publications
 Inflammation Antibodies  >83 publications with IL-6R and Inflammation
 Immune System Diseases Antibodies  >49 publications with IL-6R and Immune System Diseases
 Cardiovascular Diseases Antibodies  >42 publications with IL-6R and Cardiovascular Diseases
 Arthritis, Rheumatoid Antibodies  >28 publications with IL-6R and Arthritis, Rheumatoid
 Necrosis Antibodies  >26 publications with IL-6R and Necrosis
 Heart Diseases Antibodies  >24 publications with IL-6R and Heart Diseases
 Nervous System Diseases Antibodies  >13 publications with IL-6R and Nervous System Diseases
 Carcinoma, Hepatocellular Antibodies  >9 publications with IL-6R and Carcinoma, Hepatocellular
 Brain Diseases Antibodies  >9 publications with IL-6R and Brain Diseases
 Liver Diseases Antibodies  >8 publications with IL-6R and Liver Diseases
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >104 publications with IL-6R and Blood
 Heart Antibodies  >31 publications with IL-6R and Heart
 Vascular Antibodies  >22 publications with IL-6R and Vascular
 Liver Antibodies  >15 publications with IL-6R and Liver
 Bone Antibodies  >11 publications with IL-6R and Bone
 Lung Antibodies  >11 publications with IL-6R and Lung
 Kidney Antibodies  >10 publications with IL-6R and Kidney
 Muscle Antibodies  >9 publications with IL-6R and Muscle
 Brain Antibodies  >9 publications with IL-6R and Brain
 Prostate Antibodies  >4 publications with IL-6R and Prostate
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