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KS elisa kit :: Canine Keratan Sulfate ELISA Kit

Scan QR to view Datasheet Catalog #    MBS006090 KS elisa kit
Unit / Price
48-Strip-Wells  /  $470 +1 FREE 8GB USB
96-Strip-Wells  /  $680 +1 FREE 8GB USB
5x96-Strip-Wells  /  $3,100 +3 FREE 8GB USB
10x96-Strip-Wells  /  $6,095 +7 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Keratan Sulfate (KS), ELISA Kit

★Popular Item★
 Also Known As   

Canine Keratan Sulfate ELISA Kit

 Product Gene Name   

KS elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    603797
 3D Structure    ModBase 3D Structure for O43916
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 Species Reactivity    Canine
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 Specificity    No significant cross-reactivity or interference between Canine KS and analogues was observed.
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 Samples    Serum, Plasma, Tissue Homogenate, Feces, Urine and Body Fluids
 Assay Type    Sandwich
 Detection Range    31.2 ng/ml - 1000 ng/ml.
 Sensitivity    5.0 ng/ml.
 Intended Uses    This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated KS concentrations in Canine serum, plasma and other body fluids. Using Purified Canine KS antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add KS and KS antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of KS in the samples is then determined by comparing the O.D. of the samples to the standard curve.
 Intra-assay Precision    Intra-assay CV (%) is less than 15%.
 Inter-assay Precision    Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean 100]
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 Preparation and Storage    Store all reagents at 2-8 degree C
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of KS elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for KS purchase    MBS006090 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Keratan Sulfate (KS) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 31.2 ng/ml - 1000 ng/ml in biological research samples containing KS, with an estimated sensitivity of 5.0 ng/ml. The ELISA analytical biochemical technique of the MBS006090 kit is based on KS antibody-KS antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect KS antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, KS. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, Plasma, Tissue Homogenate, Feces, Urine and Body Fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for KS elisa kit

   Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated KS concentrations in Canine serum, plasma and other body fluids. Using Purified Canine KS antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add KS and KS antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of KS in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Sample Manual Insert of MBS006090. Click to request current manual
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NCBI/Uniprot data below describe general gene information for KS. It may not necessarily be applicable to this product.
 NCBI GI #    2887403
 NCBI GeneID    8534
 NCBI Accession #    BAA24840.1 [Other Products]
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 UniProt Primary Accession #    O43916 [Other Products]
 UniProt Secondary Accession #    D3DQP2 [Other Products]
 UniProt Related Accession #    O43916 [Other Products]
 Molecular Weight    46,715 Da
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 NCBI Official Full Name    keratan sulfate Gal-6-sulfotransferase
 NCBI Official Synonym Full Names    carbohydrate (keratan sulfate Gal-6) sulfotransferase 1
 NCBI Official Symbol    CHST1 [Similar Products]
 NCBI Official Synonym Symbols   
C6ST; KSST; GST-1; KS6ST; KSGal6ST
[Similar Products]
 NCBI Protein Information    carbohydrate sulfotransferase 1; carbohydrate (chondroitin 6/keratan) sulfotransferase 1; galactose/N-acetylglucosamine/N-acetylglucosamine 6-O-sulfotransferase 1
 UniProt Protein Name    Carbohydrate sulfotransferase 1
 UniProt Synonym Protein Names   
Galactose/N-acetylglucosamine/N-acetylglucosamine 6-O-sulfotransferase 1; GST-1; Keratan sulfate Gal-6 sulfotransferase
 UniProt Gene Name    CHST1 [Similar Products]
 UniProt Synonym Gene Names    GST-1; KS6ST; KSGal6ST; KSST [Similar Products]
 UniProt Entry Name    CHST1_HUMAN
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 NCBI Summary for KS    This locus encodes a member of the keratin sulfotransferase family of proteins. The encoded enzyme catalyzes the sulfation of the proteoglycan keratin. [provided by RefSeq, Aug 2011]
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 UniProt Comments for KS    CHST1: Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) as sulfonate donor to catalyze the transfer of sulfate to position 6 of galactose (Gal) residues of keratan. Has a preference for sulfating keratan sulfate, but it also transfers sulfate to the unsulfated polymer. The sulfotransferase activity on sialyl LacNAc structures is much higher than the corresponding desialylated substrate, and only internal Gal residues are sulfated. May function in the sulfation of sialyl N- acetyllactosamine oligosaccharide chains attached to glycoproteins. Participates in biosynthesis of selectin ligands. Selectin ligands are present in high endothelial cells (HEVs) and play a central role in lymphocyte homing at sites of inflammation. Belongs to the sulfotransferase 1 family. Gal/GlcNAc/GalNAc subfamily.

Protein type: Membrane protein, integral; Transferase; Glycan Metabolism - keratan sulfate biosynthesis; EC 2.8.2.21

Chromosomal Location of Human Ortholog: 11p11.2

Cellular Component: Golgi membrane; integral to membrane

Molecular Function: sulfotransferase activity; keratan sulfotransferase activity

Biological Process: keratan sulfate metabolic process; sulfur metabolic process; glycosaminoglycan metabolic process; keratan sulfate biosynthetic process; carbohydrate metabolic process; galactose metabolic process; polysaccharide metabolic process; pathogenesis; inflammatory response
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 Research Articles on KS    1. found endogenous expression of the long form of the enzyme in human tissue, predominantly in the trans-Golgi network of endothelial cells that form human high endothelial venules.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with KS elisa kitPathways associated with KS elisa kit
 Reference Product  PubMed Publications
 CHST2 elisa kit  >4 publications with KS and CHST2
 CHST5 elisa kit  >1 publications with KS and CHST5
 Products by Pathway  Pathway Diagram
 Disease Pathway antibodies  Disease Pathway Diagram
 Glycogen Storage Diseases Pathway antibodies  Glycogen Storage Diseases Pathway Diagram
 Glycosaminoglycan Biosynthesis - Keratan Sulfate Pathway antibodies  Glycosaminoglycan Biosynthesis - Keratan Sulfate Pathway Diagram
 Glycosaminoglycan Biosynthesis - Keratan Sulfate Pathway antibodies  Glycosaminoglycan Biosynthesis - Keratan Sulfate Pathway Diagram
 Glycosaminoglycan Metabolism Pathway antibodies  Glycosaminoglycan Metabolism Pathway Diagram
 Keratan Sulfate Biosynthesis Pathway antibodies  Keratan Sulfate Biosynthesis Pathway Diagram
 Keratan Sulfate/keratin Metabolism Pathway antibodies  Keratan Sulfate/keratin Metabolism Pathway Diagram
 MPS I - Hurler Syndrome Pathway antibodies  MPS I - Hurler Syndrome Pathway Diagram
 MPS II - Hunter Syndrome Pathway antibodies  MPS II - Hunter Syndrome Pathway Diagram
 MPS IIIA - Sanfilippo Syndrome A Pathway antibodies  MPS IIIA - Sanfilippo Syndrome A Pathway Diagram
Diseases associated with KS elisa kitOrgans/Tissues associated with KS elisa kit
 Disease Name  Pubmed Publications
 Inflammation Antibodies  >2 publications with KS and Inflammation
 Ovarian Neoplasms Antibodies  >1 publications with KS and Ovarian Neoplasms
 Ovarian Diseases Antibodies  >1 publications with KS and Ovarian Diseases
 Organ/Tissue Name  Pubmed Publications
 Eye Antibodies  >1 publications with KS and Eye
 Lung Antibodies  >1 publications with KS and Lung
 Brain Antibodies  >1 publications with KS and Brain
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