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MCP-1 elisa kit :: Canine Monocyte chemotactic protein 1/monocyte chemotactic and activating factor ELISA Kit

Scan QR to view Datasheet Catalog #    MBS734688
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $440 +1 FREE 8GB USB
96-Strip-Wells  /  $640 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,895 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,415 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Monocyte chemotactic protein 1/monocyte chemotactic and activating factor (MCP-1), ELISA Kit

★Popular Item★
 Also Known As   

Canine Monocyte chemotactic protein 1/monocyte chemotactic and activating factor ELISA Kit

 Product Gene Name   

MCP-1 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    gene 607948
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 Species Reactivity    Canine
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 Specificity    This assay has high sensitivity and excellent specificity for detection of MCP-1. No significant cross-reactivity or interference between MCP-1 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between MCP-1 and all the analogues, therefore, cross reaction may still exist in some cases.
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 Samples    Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
 Assay Type    Sandwich
 Detection Range    50-1000pg/mL
 Sensitivity    1.0pg/mL
 Intended Uses    This MCP-1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Canine MCP-1. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
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 Preparation and Storage    Store all reagents at 2-8 degree C.
 Sample Preparation    We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of MCP-1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for MCP-1 purchase    MBS734688 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Monocyte chemotactic protein 1/monocyte chemotactic and activating factor (MCP-1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 50-1000pg/mL in biological research samples containing MCP-1, with an estimated sensitivity of 1.0pg/mL. The ELISA analytical biochemical technique of the MBS734688 kit is based on MCP-1 antibody-MCP-1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect MCP-1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, MCP-1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for MCP-1 elisa kit

   Principle of the assay: MCP-1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-MCP-1 antibody and an MCP-1-HRP conjugate. The assay sample and buffer are incubated together with MCP-1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the MCP-1 concentration since MCP-1 from samples and MCP-1-HRP conjugate compete for the anti-MCP-1 antibody binding site. Since the number of sites is limited, as more sites are occupied by MCP-1 from the sample, fewer sites are left to bind MCP-1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The MCP-1 concentration in each sample is interpolated from this standard curve.
 Product Categories/Family for MCP-1 elisa kit    Immunology
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 Typical Testing Data/Standard Curve (for reference only) of MCP-1 elisa kit    MCP-1 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS734688. Click to request current manual
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NCBI/Uniprot data below describe general gene information for MCP-1. It may not necessarily be applicable to this product.
 NCBI GI #    545465
 NCBI GeneID    6347
 NCBI Accession #    AAB29926.1 [Other Products]
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 UniProt Secondary Accession #    Q9UDF3; B2R4V3 [Other Products]
 UniProt Related Accession #    P13500 [Other Products]
 Molecular Weight    11,025 Da
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 NCBI Official Full Name    MCP-1
 NCBI Official Synonym Full Names    chemokine (C-C motif) ligand 2
 NCBI Official Symbol    CCL2 [Similar Products]
 NCBI Official Synonym Symbols   
HC11; MCAF; MCP1; MCP-1; SCYA2; GDCF-2; SMC-CF; HSMCR30
[Similar Products]
 NCBI Protein Information    C-C motif chemokine 2; small-inducible cytokine A2; monocyte secretory protein JE; monocyte chemotactic protein 1; monocyte chemoattractant protein 1; monocyte chemoattractant protein-1; monocyte chemotactic and activating factor; small inducible cytokine subfamily A (Cys-Cys), member 2; small inducible cytokine A2 (monocyte chemotactic protein 1, homologous to mouse Sig-je)
 UniProt Protein Name    C-C motif chemokine 2
 UniProt Synonym Protein Names   
HC11; Monocyte chemoattractant protein 1; Monocyte chemotactic and activating factor; MCAF; Monocyte chemotactic protein 1; MCP-1; Monocyte secretory protein JE; Small-inducible cytokine A2
 Protein Family    Major capsid protein
 UniProt Gene Name    CCL2 [Similar Products]
 UniProt Synonym Gene Names    MCP1; SCYA2; MCAF; MCP-1 [Similar Products]
 UniProt Entry Name    CCL2_HUMAN
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 NCBI Summary for MCP-1    This gene is one of several cytokine genes clustered on the q-arm of chromosome 17. Chemokines are a superfamily of secreted proteins involved in immunoregulatory and inflammatory processes. The superfamily is divided into four subfamilies based on the arrangement of N-terminal cysteine residues of the mature peptide. This chemokine is a member of the CC subfamily which is characterized by two adjacent cysteine residues. This cytokine displays chemotactic activity for monocytes and basophils but not for neutrophils or eosinophils. It has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis and atherosclerosis. It binds to chemokine receptors CCR2 and CCR4. [provided by RefSeq, Jul 2013]
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 UniProt Comments for MCP-1    CCL2: Chemotactic factor that attracts monocytes and basophils but not neutrophils or eosinophils. Augments monocyte anti-tumor activity. Has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis or atherosclerosis. May be involved in the recruitment of monocytes into the arterial wall during the disease process of atherosclerosis. Monomer or homodimer; in equilibrium. Binds to CCR2 and CCR4. Is tethered on endothelial cells by glycosaminoglycan (GAG) side chains of proteoglycans. Belongs to the intercrine beta (chemokine CC) family.

Protein type: Chemokine; Motility/polarity/chemotaxis; Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 17q11.2-q12

Cellular Component: extracellular space; rough endoplasmic reticulum; perinuclear region of cytoplasm; endocytic vesicle; dendrite; extracellular region; synapse; perikaryon; nerve terminal

Molecular Function: heparin binding; CCR2 chemokine receptor binding; chemokine activity; protein kinase activity; receptor binding

Biological Process: maternal process involved in pregnancy; protein amino acid phosphorylation; response to antibiotic; regulation of cell shape; monocyte chemotaxis; cell surface receptor linked signal transduction; transforming growth factor beta receptor signaling pathway; response to vitamin B3; cellular homeostasis; negative regulation of neuron apoptosis; cell adhesion; neutrophil chemotaxis; organ regeneration; JAK-STAT cascade; positive regulation of tumor necrosis factor production; response to amino acid stimulus; G-protein signaling, coupled to cyclic nucleotide second messenger; organ morphogenesis; unfolded protein response, activation of signaling protein activity; response to ethanol; cellular response to insulin stimulus; response to bacterium; response to mechanical stimulus; response to heat; positive regulation of endothelial cell proliferation; response to activity; response to progesterone stimulus; positive regulation of nitric-oxide synthase biosynthetic process; positive regulation of collagen biosynthetic process; signal transduction; chemotaxis; positive regulation of synaptic transmission; positive regulation of cellular extravasation; response to wounding; lipopolysaccharide-mediated signaling pathway; protein kinase B signaling cascade; response to gamma radiation; angiogenesis; inflammatory response; lymphocyte chemotaxis; aging; unfolded protein response; cytokine and chemokine mediated signaling pathway; MAPKKK cascade; cytoskeleton organization and biogenesis; viral genome replication; macrophage chemotaxis; leukocyte migration during inflammatory response; humoral immune response; cellular calcium ion homeostasis; G-protein coupled receptor protein signaling pathway; positive regulation of leukocyte mediated cytotoxicity; negative regulation of angiogenesis; cellular protein metabolic process; maternal process involved in parturition; response to hypoxia; positive regulation of T cell activation; vascular endothelial growth factor receptor signaling pathway; astrocyte cell migration

Disease: Neural Tube Defects; Mycobacterium Tuberculosis, Susceptibility To; Human Immunodeficiency Virus Type 1, Susceptibility To
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 Research Articles on MCP-1    1. MM cells increased production of MCP-1 from BMSCs and BMSC-derived MCP-1 enhanced OC formation.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with MCP-1 elisa kitPathways associated with MCP-1 elisa kit
 Reference Product  PubMed Publications
 CCR2 elisa kit  >1208 publications with MCP-1 and CCR2
 JUN elisa kit  >1167 publications with MCP-1 and JUN
 CCR5 elisa kit  >325 publications with MCP-1 and CCR5
 IL6 elisa kit  >255 publications with MCP-1 and IL6
 CCR1 elisa kit  >179 publications with MCP-1 and CCR1
 CXCR4 elisa kit  >143 publications with MCP-1 and CXCR4
 CX3CR1 elisa kit  >114 publications with MCP-1 and CX3CR1
 CCR3 elisa kit  >100 publications with MCP-1 and CCR3
 JAK2 elisa kit  >26 publications with MCP-1 and JAK2
 DARC elisa kit  >21 publications with MCP-1 and DARC
 Products by Pathway  Pathway Diagram
 ATF4 Activates Genes Pathway antibodies  ATF4 Activates Genes Pathway Diagram
 Chagas Disease (American Trypanosomiasis) Pathway antibodies  Chagas Disease (American Trypanosomiasis) Pathway Diagram
 Chagas Disease (American Trypanosomiasis) Pathway antibodies  Chagas Disease (American Trypanosomiasis) Pathway Diagram
 Chemokine Receptors Bind Chemokines Pathway antibodies  Chemokine Receptors Bind Chemokines Pathway Diagram
 Chemokine Signaling Pathway antibodies  Chemokine Signaling Pathway Diagram
 Chemokine Signaling Pathway antibodies  Chemokine Signaling Pathway Diagram
 Class A/1 (Rhodopsin-like Receptors) Pathway antibodies  Class A/1 (Rhodopsin-like Receptors) Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 GPCR Ligand Binding Pathway antibodies  GPCR Ligand Binding Pathway Diagram
Diseases associated with MCP-1 elisa kitOrgans/Tissues associated with MCP-1 elisa kit
 Disease Name  Pubmed Publications
 Inflammation Antibodies  >4239 publications with MCP-1 and Inflammation
 Necrosis Antibodies  >2487 publications with MCP-1 and Necrosis
 Cardiovascular Diseases Antibodies  >1818 publications with MCP-1 and Cardiovascular Diseases
 Atherosclerosis Antibodies  >1014 publications with MCP-1 and Atherosclerosis
 Myocardial Ischemia Antibodies  >390 publications with MCP-1 and Myocardial Ischemia
 Liver Diseases Antibodies  >338 publications with MCP-1 and Liver Diseases
 Hypersensitivity Antibodies  >318 publications with MCP-1 and Hypersensitivity
 Glomerulonephritis Antibodies  >283 publications with MCP-1 and Glomerulonephritis
 Lung Injury Antibodies  >242 publications with MCP-1 and Lung Injury
 Asthma Antibodies  >209 publications with MCP-1 and Asthma
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >5504 publications with MCP-1 and Blood
 Vascular Antibodies  >2496 publications with MCP-1 and Vascular
 Kidney Antibodies  >1130 publications with MCP-1 and Kidney
 Lung Antibodies  >1062 publications with MCP-1 and Lung
 Muscle Antibodies  >1004 publications with MCP-1 and Muscle
 Brain Antibodies  >906 publications with MCP-1 and Brain
 Bone Antibodies  >692 publications with MCP-1 and Bone
 Liver Antibodies  >648 publications with MCP-1 and Liver
 Heart Antibodies  >638 publications with MCP-1 and Heart
 Bone Marrow Antibodies  >458 publications with MCP-1 and Bone Marrow
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