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ME1 elisa kit :: Canine NADP-dependent malic enzyme (ME1) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS7200810 ME1 elisa kit
Unit / Price
48-Strip-Wells  /  $440 +1 FREE 8GB USB
96-Strip-Wells  /  $640 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,895 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,415 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

NADP-dependent malic enzyme (ME1), ELISA Kit

 Also Known As   

Canine NADP-dependent malic enzyme (ME1) ELISA Kit

 Product Gene Name   

ME1 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    154250
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 Species Reactivity    Canine
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 Samples    Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
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 Preparation and Storage    Store all reagents at 2-8 degree C.
 Sample Preparation    We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of ME1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for ME1 purchase    MBS7200810 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the NADP-dependent malic enzyme (ME1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing ME1. The ELISA analytical biochemical technique of the MBS7200810 kit is based on ME1 antibody-ME1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect ME1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, ME1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Sample Manual Insert of MBS7200810. Click to request current manual
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NCBI/Uniprot data below describe general gene information for ME1. It may not necessarily be applicable to this product.
 NCBI GI #    4505143
 NCBI GeneID    4199
 NCBI Accession #    NP_002386.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_002395.5 [Other Products]
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 UniProt Secondary Accession #    Q16797; Q16855; Q53F72; Q5VWA2; Q9BWX8; Q9H1W3; Q9UIY4 [Other Products]
 UniProt Related Accession #    P48163 [Other Products]
 Molecular Weight    64,150 Da
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 NCBI Official Full Name    NADP-dependent malic enzyme
 NCBI Official Synonym Full Names    malic enzyme 1, NADP(+)-dependent, cytosolic
 NCBI Official Symbol    ME1 [Similar Products]
 NCBI Official Synonym Symbols   
MES; HUMNDME
[Similar Products]
 NCBI Protein Information    NADP-dependent malic enzyme; NADP-ME; malate dehydrogenase; malic enzyme 1, soluble; Malic enzyme, cytoplasmic; pyruvic-malic carboxylase
 UniProt Protein Name    NADP-dependent malic enzyme
 UniProt Synonym Protein Names   
Malic enzyme 1
 Protein Family    NADP-dependent malic enzyme
 UniProt Gene Name    ME1 [Similar Products]
 UniProt Synonym Gene Names    NADP-ME [Similar Products]
 UniProt Entry Name    MAOX_HUMAN
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 NCBI Summary for ME1    This gene encodes a cytosolic, NADP-dependent enzyme that generates NADPH for fatty acid biosynthesis. The activity of this enzyme, the reversible oxidative decarboxylation of malate, links the glycolytic and citric acid cycles. The regulation of expression for this gene is complex. Increased expression can result from elevated levels of thyroid hormones or by higher proportions of carbohydrates in the diet. [provided by RefSeq, Jul 2008]
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 UniProt Comments for ME1    ME1: a ubiquitous lipogenic enzyme of the malic enzymes family. Converts (S)-malate + NADP+ ?? pyruvate + CO2 + NADPH. Levels of ME1 and ATP-citrate lyase mRNA, another lipogenic enzyme, are coordinately increased 5-7 fold during the differentiation of 3T3-L1 preadipocytes into adipocytes. Up-regulated by short-term dietary restriction in mice. The cycling of pyruvate by ME1 generates cytosolic NADPH, whereas mitochondrial ME2 responds to elevated amino acids and serves to supply sufficient pyruvate for increased Krebs cycle flux when glucose is limiting.

Protein type: Oxidoreductase; Carbohydrate Metabolism - pyruvate; EC 1.1.1.40

Chromosomal Location of Human Ortholog: 6q12

Cellular Component: mitochondrion; cytosol

Molecular Function: malate dehydrogenase (decarboxylating) activity; malic enzyme activity; electron carrier activity; manganese ion binding; malate dehydrogenase (oxaloacetate-decarboxylating) (NADP+) activity; NADP binding; ADP binding; NAD binding; oxaloacetate decarboxylase activity

Biological Process: malate metabolic process; carbohydrate metabolic process; response to hormone stimulus; NADP biosynthetic process; cellular lipid metabolic process; response to carbohydrate stimulus; protein tetramerization
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 Research Articles on ME1    1. essential role for ME1 in the production of cytosolic NADPH and maintenance of migratory and invasive abilities of nasopharyngeal carcinoma cells
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with ME1 elisa kitPathways associated with ME1 elisa kit
 Reference Product  PubMed Publications
 LDHB elisa kit  >6 publications with ME1 and LDHB
 PKM2 elisa kit  >6 publications with ME1 and PKM2
 MDH1 elisa kit  >5 publications with ME1 and MDH1
 MDH2 elisa kit  >2 publications with ME1 and MDH2
 PKLR elisa kit  >1 publications with ME1 and PKLR
 Products by Pathway  Pathway Diagram
 Carbon Metabolism Pathway antibodies  Carbon Metabolism Pathway Diagram
 Carbon Metabolism Pathway antibodies  Carbon Metabolism Pathway Diagram
 Fatty Acid, Triacylglycerol, And Ketone Body Metabolism Pathway antibodies  Fatty Acid, Triacylglycerol, And Ketone Body Metabolism Pathway Diagram
 Metabolic Pathways antibodies  Metabolic Pathways Diagram
 Metabolism Pathway antibodies  Metabolism Pathway Diagram
 Metabolism Of Lipids And Lipoproteins Pathway antibodies  Metabolism Of Lipids And Lipoproteins Pathway Diagram
 PPAR Signaling Pathway antibodies  PPAR Signaling Pathway Diagram
 PPAR Signaling Pathway antibodies  PPAR Signaling Pathway Diagram
 PPARA Activates Gene Expression Pathway antibodies  PPARA Activates Gene Expression Pathway Diagram
 Pyruvate Metabolism Pathway antibodies  Pyruvate Metabolism Pathway Diagram
Diseases associated with ME1 elisa kitOrgans/Tissues associated with ME1 elisa kit
 Disease Name  Pubmed Publications
 Liver Diseases Antibodies  >11 publications with ME1 and Liver Diseases
 Disease Models, Animal Antibodies  >11 publications with ME1 and Disease Models, Animal
 Obesity Antibodies  >8 publications with ME1 and Obesity
 Fatty Liver Antibodies  >6 publications with ME1 and Fatty Liver
 Neoplasms, Experimental Antibodies  >6 publications with ME1 and Neoplasms, Experimental
 Liver Neoplasms Antibodies  >5 publications with ME1 and Liver Neoplasms
 Adenocarcinoma Antibodies  >5 publications with ME1 and Adenocarcinoma
 Nervous System Diseases Antibodies  >5 publications with ME1 and Nervous System Diseases
 Lung Neoplasms Antibodies  >5 publications with ME1 and Lung Neoplasms
 Breast Neoplasms Antibodies  >4 publications with ME1 and Breast Neoplasms
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >35 publications with ME1 and Blood
 Liver Antibodies  >21 publications with ME1 and Liver
 Muscle Antibodies  >13 publications with ME1 and Muscle
 Skin Antibodies  >8 publications with ME1 and Skin
 Brain Antibodies  >8 publications with ME1 and Brain
 Connective Tissue Antibodies  >8 publications with ME1 and Connective Tissue
 Lung Antibodies  >6 publications with ME1 and Lung
 Pancreas Antibodies  >5 publications with ME1 and Pancreas
 Kidney Antibodies  >4 publications with ME1 and Kidney
 Heart Antibodies  >4 publications with ME1 and Heart
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