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PCSK9 elisa kit :: Canine Proprotein Convertase Subtilisin/Kexin Type 9 ELISA Kit

Scan QR to view Datasheet Catalog #    MBS751361
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $470 +1 FREE 8GB USB
96-Strip-Wells  /  $675 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,925 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,490 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Proprotein Convertase Subtilisin/Kexin Type 9 (PCSK9), ELISA Kit

★Popular Item★
 Also Known As   

Canine Proprotein Convertase Subtilisin/Kexin Type 9 ELISA Kit

 Product Gene Name   

PCSK9 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    607786
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 Species Reactivity    Canine
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 Specificity    This assay has high sensitivity and excellent specificity for detection of PCSK-9. No significant cross-reactivity or interference between PCSK-9 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between PCSK-9 and all the analogues, therefore, cross reaction may still exist in some cases.
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 Samples    Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
 Assay Type    Competitive
 Sensitivity    1.0 pg/mL.
 Intended Uses    This PCSK-9 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Canine PCSK-9. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
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 Preparation and Storage    Store all reagents at 2-8 degree C.
 Sample Preparation    We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of PCSK9 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for PCSK9 purchase    MBS751361 is a ready-to-use microwell, strip plate Competitive ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Proprotein Convertase Subtilisin/Kexin Type 9 (PCSK9) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing PCSK9, with an estimated sensitivity of 1.0 pg/mL. The ELISA analytical biochemical technique of the MBS751361 kit is based on PCSK9 antibody-PCSK9 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect PCSK9 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, PCSK9. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Product Description specifically for PCSK9 elisa kit

   Principle of the assay: PCSK-9 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-PCSK-9 antibody and an PCSK-9-HRP conjugate. The assay sample and buffer are incubated together with PCSK-9-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the PCSK-9 concentration since PCSK-9 from samples and PCSK-9-HRP conjugate compete for the anti-PCSK-9 antibody binding site. Since the number of sites is limited, as more sites are occupied by PCSK-9 from the sample, fewer sites are left to bind PCSK-9-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PCSK-9 concentration in each sample is interpolated from this standard curve.
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 Typical Testing Data/Standard Curve (for reference only) of PCSK9 elisa kit    PCSK9 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS751361. Click to request current manual
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NCBI/Uniprot data below describe general gene information for PCSK9. It may not necessarily be applicable to this product.
 NCBI GI #    31317307
 NCBI GeneID    255738
 NCBI Accession #    NP_777596.2 [Other Products]
 NCBI GenBank Nucleotide #    NM_174936.3 [Other Products]
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 UniProt Secondary Accession #    Q5PSM5; Q5SZQ2; A8T640; C0JYY9 [Other Products]
 UniProt Related Accession #    Q8NBP7 [Other Products]
 Molecular Weight    20,827 Da
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 NCBI Official Full Name    proprotein convertase subtilisin/kexin type 9 preproprotein
 NCBI Official Synonym Full Names    proprotein convertase subtilisin/kexin type 9
 NCBI Official Symbol    PCSK9 [Similar Products]
 NCBI Official Synonym Symbols   
FH3; PC9; NARC1; LDLCQ1; NARC-1; HCHOLA3
[Similar Products]
 NCBI Protein Information    proprotein convertase subtilisin/kexin type 9; subtilisin/kexin-like protease PC9; neural apoptosis regulated convertase 1; convertase subtilisin/kexin type 9 preproprotein
 UniProt Protein Name    Proprotein convertase subtilisin/kexin type 9
 UniProt Synonym Protein Names   
Neural apoptosis-regulated convertase 1; NARC-1; Proprotein convertase 9; PC9; Subtilisin/kexin-like protease PC9
 Protein Family    Proprotein convertase subtilisin/kexin
 UniProt Gene Name    PCSK9 [Similar Products]
 UniProt Synonym Gene Names    NARC1; PSEC0052; NARC-1; PC9 [Similar Products]
 UniProt Entry Name    PCSK9_HUMAN
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 NCBI Summary for PCSK9    This gene encodes a member of the subtilisin-like proprotein convertase family, which includes proteases that process protein and peptide precursors trafficking through regulated or constitutive branches of the secretory pathway. The encoded protein undergoes an autocatalytic processing event with its prosegment in the ER and is constitutively secreted as an inactive protease into the extracellular matrix and trans-Golgi network. It is expressed in liver, intestine and kidney tissues and escorts specific receptors for lysosomal degradation. It plays a role in cholesterol and fatty acid metabolism. Mutations in this gene have been associated with autosomal dominant familial hypercholesterolemia. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2014]
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 UniProt Comments for PCSK9    PCSK9: Crucial player in the regulation of plasma cholesterol homeostasis. Binds to low-density lipid receptor family members: low density lipoprotein receptor (LDLR), very low density lipoprotein receptor (VLDLR), apolipoprotein E receptor (LRP1/APOER) and apolipoprotein receptor 2 (LRP8/APOER2), and promotes their degradation in intracellular acidic compartments. Acts via a non-proteolytic mechanism to enhance the degradation of the hepatic LDLR through a clathrin LDLRAP1/ARH-mediated pathway. May prevent the recycling of LDLR from endosomes to the cell surface or direct it to lysosomes for degradation. Can induce ubiquitination of LDLR leading to its subsequent degradation. Inhibits intracellular degradation of APOB via the autophagosome/lysosome pathway in a LDLR-independent manner. Involved in the disposal of non-acetylated intermediates of BACE1 in the early secretory pathway. Inhibits epithelial Na(+) channel (ENaC)-mediated Na(+) absorption by reducing ENaC surface expression primarily by increasing its proteasomal degradation. Regulates neuronal apoptosis via modulation of LRP8/APOER2 levels and related anti-apoptotic signaling pathways. Defects in PCSK9 are the cause of hypercholesterolemia autosomal dominant type 3 (HCHOLA3). A familial condition characterized by elevated circulating cholesterol contained in either low-density lipoproteins alone or also in very-low-density lipoproteins. Belongs to the peptidase S8 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.4.21.-; Cell development/differentiation; Secreted; Secreted, signal peptide; Protease

Chromosomal Location of Human Ortholog: 1p32.3

Cellular Component: Golgi apparatus; extracellular space; cell surface; rough endoplasmic reticulum; endoplasmic reticulum; lysosome; early endosome; ER to Golgi transport vesicle; extrinsic to external side of plasma membrane; perinuclear region of cytoplasm; late endosome; cytoplasm; plasma membrane

Molecular Function: very-low-density lipoprotein binding; sodium channel inhibitor activity; protein binding; protein self-association; low-density lipoprotein receptor binding; serine-type endopeptidase activity; low-density lipoprotein binding; apolipoprotein binding; apolipoprotein receptor binding

Biological Process: cholesterol metabolic process; lysosomal transport; apoptosis; positive regulation of receptor internalization; lipoprotein metabolic process; regulation of low-density lipoprotein receptor catabolic process; cellular response to starvation; proteolysis; liver development; neuron differentiation; protein autoprocessing; cholesterol homeostasis; triacylglycerol metabolic process; neurogenesis; cellular response to insulin stimulus; phospholipid metabolic process; positive regulation of neuron apoptosis; regulation of neuron apoptosis; negative regulation of receptor recycling; low-density lipoprotein receptor catabolic process; regulation of receptor activity; kidney development

Disease: Hypercholesterolemia, Autosomal Dominant, 3
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 Research Articles on PCSK9    1. Elevated plasma PCSK9 level is equally detrimental for patients with nonfamilial hypercholesterolemia and heterozygous familial hypercholesterolemia, irrespective of low-density lipoprotein receptor defects
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with PCSK9 elisa kitDiseases associated with PCSK9 elisa kit
 Reference Product  PubMed Publications
 LDLR elisa kit  >302 publications with PCSK9 and LDLR
 APOB elisa kit  >181 publications with PCSK9 and APOB
 SREBF2 elisa kit  >10 publications with PCSK9 and SREBF2
 VLDLR elisa kit  >9 publications with PCSK9 and VLDLR
 Disease Name  Pubmed Publications
 Hypercholesterolemia Antibodies  >364 publications with PCSK9 and Hypercholesterolemia
 Dyslipidemias Antibodies  >316 publications with PCSK9 and Dyslipidemias
 Hyperlipidemias Antibodies  >267 publications with PCSK9 and Hyperlipidemias
 Atherosclerosis Antibodies  >187 publications with PCSK9 and Atherosclerosis
 Hypercholesterolemia, Autosomal Dominant, 3 Antibodies  >59 publications with PCSK9 and Hypercholesterolemia, Autosomal Dominant, 3
 Coronary Artery Disease Antibodies  >57 publications with PCSK9 and Coronary Artery Disease
 Myocardial Infarction Antibodies  >28 publications with PCSK9 and Myocardial Infarction
 Liver Diseases Antibodies  >23 publications with PCSK9 and Liver Diseases
 Fatty Liver Antibodies  >18 publications with PCSK9 and Fatty Liver
 Inflammation Antibodies  >17 publications with PCSK9 and Inflammation
Organs/Tissues associated with PCSK9 elisa kit
 Organ/Tissue Name  Pubmed Publications
 Liver Antibodies  >168 publications with PCSK9 and Liver
 Intestine Antibodies  >15 publications with PCSK9 and Intestine
 Brain Antibodies  >14 publications with PCSK9 and Brain
 Skin Antibodies  >5 publications with PCSK9 and Skin
 Embryonic Tissue Antibodies  >4 publications with PCSK9 and Embryonic Tissue
 Ovary Antibodies  >3 publications with PCSK9 and Ovary
 Connective Tissue Antibodies  >3 publications with PCSK9 and Connective Tissue
 Stomach Antibodies  >2 publications with PCSK9 and Stomach
 Pancreas Antibodies  >2 publications with PCSK9 and Pancreas
 Bone Antibodies  >1 publications with PCSK9 and Bone
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