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SDH elisa kit :: Canine Sorbitol Dehydrogenase ELISA Kit

Scan QR to view Datasheet Catalog #    MBS2603115
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $300 +1 FREE 8GB USB
96-Strip-Wells  /  $475 +1 FREE 8GB USB
5x96-Strip-Wells  /  $1,825 +2 FREE 8GB USB
10x96-Strip-Wells  /  $3,425 +4 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Sorbitol Dehydrogenase (SDH), ELISA Kit

★Popular Item★
 Also Known As   

Canine Sorbitol Dehydrogenase (SDH) ELISA Kit

 Product Gene Name   

SDH elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    182500
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 Species Reactivity    Canine
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 Specificity    No cross-reaction with other factors.
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 Samples    Canine serum, plasma or cell culture supernatant and organizations
 Assay Type    Quantitative Sandwich
 Detection Range    20 ng/ml-0.312 ng/ml
 Sensitivity    0.06 ng/ml
 Intra-assay Precision    <= 8%
 Inter-assay Precision    <= 12%
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 Preparation and Storage    Store all reagents at 2-8 degree C.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of SDH elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for SDH purchase    MBS2603115 is a ready-to-use microwell, strip plate Quantitative Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Sorbitol Dehydrogenase (SDH) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 20 ng/ml-0.312 ng/ml in biological research samples containing SDH, with an estimated sensitivity of 0.06 ng/ml. The ELISA analytical biochemical technique of the MBS2603115 kit is based on SDH antibody-SDH antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect SDH antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, SDH. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Canine serum, plasma or cell culture supernatant and organizations. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for SDH elisa kit

   Principle of the Assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Canine SDH monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.
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 Typical Testing Data/Standard Curve (for reference only) of SDH elisa kit    SDH elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS2603115. Click to request current manual
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NCBI/Uniprot data below describe general gene information for SDH. It may not necessarily be applicable to this product.
 NCBI GI #    156627571
 NCBI GeneID    6652
 NCBI Accession #    NP_003095.2 [Other Products]
 NCBI GenBank Nucleotide #    NM_003104.5 [Other Products]
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 UniProt Secondary Accession #    Q16682; Q9UMD6; B2R655; B7Z3A6; J3JZZ5 [Other Products]
 UniProt Related Accession #    Q00796 [Other Products]
 Molecular Weight    10,812 Da
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 NCBI Official Full Name    sorbitol dehydrogenase
 NCBI Official Synonym Full Names    sorbitol dehydrogenase
 NCBI Official Symbol    SORD [Similar Products]
 NCBI Official Synonym Symbols   
SORD1; HEL-S-95n
[Similar Products]
 NCBI Protein Information    sorbitol dehydrogenase
 UniProt Protein Name    Sorbitol dehydrogenase
 UniProt Synonym Protein Names   
L-iditol 2-dehydrogenase
 Protein Family    Serine 3-dehydrogenase
 UniProt Gene Name    SORD [Similar Products]
 UniProt Entry Name    DHSO_HUMAN
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 NCBI Summary for SDH    Sorbitol dehydrogenase (SORD; EC 1.1.1.14) catalyzes the interconversion of polyols and their corresponding ketoses, and together with aldose reductase (ALDR1; MIM 103880), makes up the sorbitol pathway that is believed to play an important role in the development of diabetic complications (summarized by Carr and Markham, 1995 [PubMed 8535074]). The first reaction of the pathway (also called the polyol pathway) is the reduction of glucose to sorbitol by ALDR1 with NADPH as the cofactor. SORD then oxidizes the sorbitol to fructose using NAD(+) cofactor.[supplied by OMIM, Jul 2010]
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 UniProt Comments for SDH    SORD: Converts sorbitol to fructose. Part of the polyol pathway that plays an important role in sperm physiology. May play a role in the sperm motility by providing an energetic source for sperm. Belongs to the zinc-containing alcohol dehydrogenase family.

Protein type: EC 1.1.1.14; Oxidoreductase; Carbohydrate Metabolism - fructose and mannose

Chromosomal Location of Human Ortholog: 15q15.3

Cellular Component: cytosol; extracellular space; membrane; mitochondrial membrane

Molecular Function: carbohydrate binding; D-xylulose reductase activity; identical protein binding; L-iditol 2-dehydrogenase activity; NAD binding; zinc ion binding

Biological Process: carbohydrate metabolic process; fructose biosynthetic process; fructose metabolic process; glucose metabolic process; glucuronate catabolic process to xylulose 5-phosphate; L-xylitol catabolic process; L-xylitol metabolic process; response to cadmium ion; response to copper ion; response to drug; response to hormone stimulus; response to nutrient levels; response to osmotic stress; sorbitol catabolic process; sperm motility
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 Research Articles on SDH    1. One of the most striking changes involved sorbitol dehydrogenase, a key enzyme in the polyol pathway. Validation studies revealed dramatically increased sorbitol dehydrogenase concentrations and activity in adenomas and cancer cell lines, along with important changes in the expression of other enzymes in the same (AKR1B1) and related (KHK) pathways.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with SDH elisa kitPathways associated with SDH elisa kit
 Reference Product  PubMed Publications
 HK1 elisa kit  >1 publications with SDH and HK1
 Products by Pathway  Pathway Diagram
 Catabolism Of Glucuronate To Xylulose-5-phosphate Pathway antibodies  Catabolism Of Glucuronate To Xylulose-5-phosphate Pathway Diagram
 Fructose And Mannose Metabolism Pathway antibodies  Fructose And Mannose Metabolism Pathway Diagram
 Fructose And Mannose Metabolism Pathway antibodies  Fructose And Mannose Metabolism Pathway Diagram
 Fructose Biosynthesis Pathway antibodies  Fructose Biosynthesis Pathway Diagram
 Fructose Metabolism Pathway antibodies  Fructose Metabolism Pathway Diagram
 Glucuronate Pathway (uronate Pathway) antibodies  Glucuronate Pathway (uronate Pathway) Diagram
 Glucuronate Pathway (uronate Pathway) antibodies  Glucuronate Pathway (uronate Pathway) Diagram
 Metabolic Pathways antibodies  Metabolic Pathways Diagram
 Metabolism Pathway antibodies  Metabolism Pathway Diagram
 Metabolism Of Carbohydrates Pathway antibodies  Metabolism Of Carbohydrates Pathway Diagram
Diseases associated with SDH elisa kitOrgans/Tissues associated with SDH elisa kit
 Disease Name  Pubmed Publications
 Liver Diseases Antibodies  >2 publications with SDH and Liver Diseases
 Neoplasms, Experimental Antibodies  >1 publications with SDH and Neoplasms, Experimental
 Carcinoma, Hepatocellular Antibodies  >1 publications with SDH and Carcinoma, Hepatocellular
 Lung Neoplasms Antibodies  >1 publications with SDH and Lung Neoplasms
 Liver Neoplasms Antibodies  >1 publications with SDH and Liver Neoplasms
 Kidney Diseases Antibodies  >1 publications with SDH and Kidney Diseases
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >12 publications with SDH and Blood
 Brain Antibodies  >8 publications with SDH and Brain
 Eye Antibodies  >4 publications with SDH and Eye
 Kidney Antibodies  >3 publications with SDH and Kidney
 Liver Antibodies  >3 publications with SDH and Liver
 Vascular Antibodies  >2 publications with SDH and Vascular
 Muscle Antibodies  >2 publications with SDH and Muscle
 Larynx Antibodies  >1 publications with SDH and Larynx
 Testis Antibodies  >1 publications with SDH and Testis
 Thyroid Antibodies  >1 publications with SDH and Thyroid
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