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ULBP2 elisa kit :: Canine UL16 Binding protein 2 ELISA Kit

Scan QR to view Datasheet Catalog #    MBS734130
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $440 +1 FREE 8GB USB
96-Strip-Wells  /  $640 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,895 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,415 +6 FREE 8GB USB
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 Product Name   

UL16 Binding protein 2 (ULBP2), ELISA Kit

★Popular Item★
 Also Known As   

Canine UL16 Binding protein 2 ELISA Kit

 Product Gene Name   

ULBP2 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    605698
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 Species Reactivity    Canine
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 Specificity    This assay has high sensitivity and excellent specificity for detection of ULBP2. No significant cross-reactivity or interference between ULBP2 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between ULBP2 and all the analogues, therefore, cross reaction may still exist in some cases.
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 Samples    Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
 Assay Type    Sandwich
 Detection Range    0.5-10ng/mL
 Sensitivity    0.1ng/mL
 Intended Uses    This ULBP2 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Canine ULBP2. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
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 Preparation and Storage    Store all reagents at 2-8 degree C.
 Sample Preparation    We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of ULBP2 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for ULBP2 purchase    MBS734130 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the UL16 Binding protein 2 (ULBP2) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 0.5-10ng/mL in biological research samples containing ULBP2, with an estimated sensitivity of 0.1ng/mL. The ELISA analytical biochemical technique of the MBS734130 kit is based on ULBP2 antibody-ULBP2 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect ULBP2 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, ULBP2. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for ULBP2 elisa kit

   Principle of the assay: ULBP2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-ULBP2 antibody and an ULBP2-HRP conjugate. The assay sample and buffer are incubated together with ULBP2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ULBP2 concentration since ULBP2 from samples and ULBP2-HRP conjugate compete for the anti-ULBP2 antibody binding site. Since the number of sites is limited, as more sites are occupied by ULBP2 from the sample, fewer sites are left to bind ULBP2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ULBP2 concentration in each sample is interpolated from this standard curve.
 Product Categories/Family for ULBP2 elisa kit    Immunology
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 Typical Testing Data/Standard Curve (for reference only) of ULBP2 elisa kit    ULBP2 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS734130. Click to request current manual
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NCBI/Uniprot data below describe general gene information for ULBP2. It may not necessarily be applicable to this product.
 NCBI GI #    21961213
 NCBI GeneID    80328
 NCBI Accession #    AAH34689.1 [Other Products]
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 UniProt Secondary Accession #    Q5VUN4 [Other Products]
 UniProt Related Accession #    Q9BZM5 [Other Products]
 Molecular Weight    27,368 Da
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 NCBI Official Full Name    UL16 binding protein 2
 NCBI Official Synonym Full Names    UL16 binding protein 2
 NCBI Official Symbol    ULBP2 [Similar Products]
 NCBI Official Synonym Symbols   
[Similar Products]
 NCBI Protein Information    NKG2D ligand 2; N2DL-2; NKG2DL2; ALCAN-alpha; UL16-binding protein 2; retinoic acid early transcript 1H; retinoic acid early transcript 1 H
 UniProt Protein Name    NKG2D ligand 2
 UniProt Synonym Protein Names   
ALCAN-alpha; Retinoic acid early transcript 1H; UL16-binding protein 2
 Protein Family    NKG2D ligand
 UniProt Gene Name    ULBP2 [Similar Products]
 UniProt Synonym Gene Names    N2DL2; RAET1H; UNQ463/PRO791; N2DL-2; NKG2DL2 [Similar Products]
 UniProt Entry Name    N2DL2_HUMAN
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 NCBI Summary for ULBP2    This gene encodes a major histocompatibility complex (MHC) class I-related molecule that binds to the NKG2D receptor on natural killer (NK) cells to trigger release of multiple cytokines and chemokines that in turn contribute to the recruitment and activation of NK cells. The encoded protein undergoes further processing to generate the mature protein that is either anchored to membrane via a glycosylphosphatidylinositol moiety, or secreted. Many malignant cells secrete the encoded protein to evade immunosurveillance by NK cells. This gene is located in a cluster of multiple MHC class I-related genes on chromosome 6. [provided by RefSeq, Jul 2015]
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 UniProt Comments for ULBP2    ULBP2: Ligand for the NKG2D receptor, together with at least ULBP1 and ULBP3. ULBPs activate multiple signaling pathways in primary NK cells, resulting in the production of cytokines and chemokines. Binding of ULBPs ligands to NKG2D induces calcium mobilization and activation of the JAK2, STAT5, ERK and PI3K kinase/Akt signal transduction pathway. In CMV infected cells, interacts with soluble CMV glycoprotein UL16. The interaction with UL16 blocked the interaction with the NKG2D receptor, providing a mechanism by which CMV infected cells might escape the immune system. UL16 also causes ULBP2 to be retained in the ER and cis- Golgi apparatus so that it does not reach the cell surface. Belongs to the MHC class I family.

Protein type: Membrane protein, GPI anchor

Chromosomal Location of Human Ortholog: 6q25

Cellular Component: extracellular space; anchored to plasma membrane; cell surface

Molecular Function: peptide antigen binding; natural killer cell lectin-like receptor binding

Biological Process: antigen processing and presentation of peptide antigen via MHC class I; natural killer cell mediated cytotoxicity; natural killer cell activation
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 Research Articles on ULBP2    1. This study provides for the first time, the c-Myc dependent regulation of NKG2D ligands, ULBP1/2/3 in acute myeloid leukemia.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with ULBP2 elisa kitPathways associated with ULBP2 elisa kit
 Reference Product  PubMed Publications
 KLRK1 elisa kit  >70 publications with ULBP2 and KLRK1
 CD226 elisa kit  >2 publications with ULBP2 and CD226
 B2M elisa kit  >1 publications with ULBP2 and B2M
 HLA-B elisa kit  >1 publications with ULBP2 and HLA-B
 IL18 elisa kit  >1 publications with ULBP2 and IL18
 Products by Pathway  Pathway Diagram
 Natural Killer Cell Mediated Cytotoxicity Pathway antibodies  Natural Killer Cell Mediated Cytotoxicity Pathway Diagram
 Natural Killer Cell Mediated Cytotoxicity Pathway antibodies  Natural Killer Cell Mediated Cytotoxicity Pathway Diagram
Diseases associated with ULBP2 elisa kitOrgans/Tissues associated with ULBP2 elisa kit
 Disease Name  Pubmed Publications
 Carcinoma Antibodies  >25 publications with ULBP2 and Carcinoma
 Lung Neoplasms Antibodies  >9 publications with ULBP2 and Lung Neoplasms
 Gastrointestinal Diseases Antibodies  >9 publications with ULBP2 and Gastrointestinal Diseases
 Necrosis Antibodies  >9 publications with ULBP2 and Necrosis
 Cardiovascular Diseases Antibodies  >7 publications with ULBP2 and Cardiovascular Diseases
 Colorectal Neoplasms Antibodies  >6 publications with ULBP2 and Colorectal Neoplasms
 Ovarian Neoplasms Antibodies  >6 publications with ULBP2 and Ovarian Neoplasms
 Nervous System Diseases Antibodies  >4 publications with ULBP2 and Nervous System Diseases
 Carcinoma, Hepatocellular Antibodies  >4 publications with ULBP2 and Carcinoma, Hepatocellular
 Stomach Neoplasms Antibodies  >4 publications with ULBP2 and Stomach Neoplasms
 Organ/Tissue Name  Pubmed Publications
 Lung Antibodies  >10 publications with ULBP2 and Lung
 Bone Antibodies  >7 publications with ULBP2 and Bone
 Skin Antibodies  >5 publications with ULBP2 and Skin
 Brain Antibodies  >3 publications with ULBP2 and Brain
 Lymph Node Antibodies  >2 publications with ULBP2 and Lymph Node
 Placenta Antibodies  >1 publications with ULBP2 and Placenta
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