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PRKAA2 elisa kit :: Chicken 5'-AMP-activated protein kinase Catalytic subunit alpha-2 ELISA Kit

Scan QR to view Datasheet Catalog #    MBS2606816 PRKAA2 elisa kit
Unit / Price
48-Strip-Wells  /  $300 +1 FREE 8GB USB
96-Strip-Wells  /  $475 +1 FREE 8GB USB
5x96-Strip-Wells  /  $1,825 +2 FREE 8GB USB
10x96-Strip-Wells  /  $3,425 +4 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

5'-AMP-activated protein kinase Catalytic subunit alpha-2 (PRKAA2), ELISA Kit

 Also Known As   

Chicken 5'-AMP-activated protein kinase Catalytic subunit alpha-2 (PRKAA2) ELISA Kit

 Product Gene Name   

PRKAA2 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Request for Current Manual Insert    Request Current Manual
 OMIM    600497
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 Species Reactivity    Chicken
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 Preparation and Storage    Store all reagents at 2-8 degree C.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of PRKAA2 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for PRKAA2 purchase    MBS2606816 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the 5'-AMP-activated protein kinase Catalytic subunit alpha-2 (PRKAA2) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing PRKAA2. The ELISA analytical biochemical technique of the MBS2606816 kit is based on PRKAA2 antibody-PRKAA2 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect PRKAA2 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, PRKAA2. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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NCBI/Uniprot data below describe general gene information for PRKAA2. It may not necessarily be applicable to this product.
 NCBI GI #    46877068
 NCBI GeneID    5563
 NCBI Accession #    NP_006243.2 [Other Products]
 NCBI GenBank Nucleotide #    NM_006252.3 [Other Products]
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 UniProt Secondary Accession #    Q9H1E8; Q9UD43 [Other Products]
 UniProt Related Accession #    P54646; AAB32732 [Other Products]
 Molecular Weight    62,320 Da
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 NCBI Official Full Name    5'-AMP-activated protein kinase catalytic subunit alpha-2
 NCBI Official Synonym Full Names    protein kinase AMP-activated catalytic subunit alpha 2
 NCBI Official Symbol    PRKAA2 [Similar Products]
 NCBI Official Synonym Symbols   
AMPK; AMPK2; PRKAA; AMPKa2
[Similar Products]
 NCBI Protein Information    5'-AMP-activated protein kinase catalytic subunit alpha-2
 UniProt Protein Name    5'-AMP-activated protein kinase catalytic subunit alpha-2
 UniProt Synonym Protein Names   
Acetyl-CoA carboxylase kinase (EC:2.7.11.27); ACACA kinase; Hydroxymethylglutaryl-CoA reductase kinase (EC:2.7.11.31); HMGCR kinase
 Protein Family    5'-AMP-activated protein kinase
 UniProt Gene Name    PRKAA2 [Similar Products]
 UniProt Synonym Gene Names    AMPK; AMPK2; AMPK subunit alpha-2; ACACA kinase; HMGCR kinase [Similar Products]
 UniProt Entry Name    AAPK2_HUMAN
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 NCBI Summary for PRKAA2    The protein encoded by this gene is a catalytic subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. Studies of the mouse counterpart suggest that this catalytic subunit may control whole-body insulin sensitivity and is necessary for maintaining myocardial energy homeostasis during ischemia. [provided by RefSeq, Jul 2008]
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 UniProt Comments for PRKAA2    AMPKA2: a catalytic subunit of AMP-activated protein kinase (AMPK). Acts as an energy sensor, playing a key role in regulating cellular energy metabolism. A protein kinase of the CAMKL family whose activation is regulated by the balance between ADP/AMP/ATP, and intracellular Ca(2+) levels. Acts as a metabolic stress-sensing protein kinase switching off biosynthetic pathways when cellular ATP levels are depleted and when 5'-ADP and -AMP rise in response to fuel limitation and/or hypoxia. Activates energy-producing pathways and inhibits energy-consuming processes. Restores ATP levels in cells by switching off anabolic and switching on catabolic pathways. Activated primarily by rising ADP levels and not, as previously thought, solely by AMP. AMPK resembles an adenylate charge regulatory system in which anabolic and catabolic pathways are regulated by adenine nucleotide ratios. Acts via direct phosphorylation of metabolic enzymes and transcription regulators. Regulates fatty acid synthesis by phosphorylating acetyl-CoA carboxylase. Regulates cholesterol synthesis by phosphorylating and inactivating hormone-sensitive lipase and hydroxymethylglutaryl-CoA reductase. Activated by at least two distinct upstream kinases: the tumor suppressor LKB1 and CaMKK2. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton, probably by indirectly activating myosin. AMPK is a heterotrimer of an alpha catalytic subunit (AMPKA1 or -2), a beta (AMPKB1 or -2) and a gamma non-catalytic subunit (AMPKG1, -2 or -3). Different possible combinations of subunits give rise to 12 different holoenzymes. Binding of ADP or AMP to non-catalytic gamma subunit (PRKAG1, -2 or -3) results in allosteric activation. AMPK is activated by antihyperglycemic drug metformin, a drug prescribed to patients with type 2 diabetes: in vivo, metformin seems to mainly inhibit liver gluconeogenesis. However, metformin can be used to activate AMPK in muscle and other cells in culture or ex vivo. Selectively inhibited by compound C (6-[4-(2-Piperidin-1-yl-ethoxy)-phenyl)]-3-pyridin-4-yl-pyyrazolo[1,5-a] pyrimidine. Activated by resveratrol, a natural polyphenol present in red wine, and S17834, a synthetic polyphenol. Salicylate/aspirin directly activates kinase activity. Studies in the mouse suggest that AMPK2 may control whole-body insulin sensitivity and is necessary for maintaining myocardial energy homeostasis during ischemia.

Protein type: Protein kinase, Ser/Thr (non-receptor); Protein kinase, CAMK; EC 2.7.11.31; Autophagy; EC 2.7.11.1; EC 2.7.11.27; Kinase, protein; CAMK group; CAMKL family; AMPK subfamily

Chromosomal Location of Human Ortholog: 1p31

Cellular Component: AMP-activated protein kinase complex; cytoplasm; cytosol; nucleoplasm

Molecular Function: [acetyl-CoA carboxylase] kinase activity; [hydroxymethylglutaryl-CoA reductase (NADPH)] kinase activity; AMP-activated protein kinase activity; ATP binding; chromatin binding; histone serine kinase activity; metal ion binding; protein binding; protein kinase activity; protein serine/threonine kinase activity; protein serine/threonine/tyrosine kinase activity

Biological Process: carnitine shuttle; cell cycle arrest; cellular lipid metabolic process; cellular response to glucose starvation; cellular response to nutrient levels; cholesterol biosynthetic process; energy reserve metabolic process; fatty acid biosynthetic process; fatty acid homeostasis; gene expression; glucose homeostasis; insulin receptor signaling pathway; lipid biosynthetic process; macroautophagy; mitochondrion organization and biogenesis; negative regulation of apoptosis; negative regulation of TOR signaling pathway; organelle organization and biogenesis; positive regulation of autophagy; positive regulation of glycolysis; positive regulation of macroautophagy; protein amino acid phosphorylation; regulation of circadian rhythm; regulation of fatty acid biosynthetic process; regulation of macroautophagy; regulation of transcription, DNA-dependent; response to muscle activity; response to stress; rhythmic process; signal transduction; transcription initiation from RNA polymerase II promoter; Wnt receptor signaling pathway
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 Research Articles on PRKAA2    1. Data (including data from studies in knockout mice) suggest AMPK activation (AMPKa1 or AMPKa2) suppresses inflammation in vascular smooth muscle cells by down-regulation of STAT1 signaling and up-regulation of MKP-1 (dual specificity phosphatase 1).
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with PRKAA2 elisa kitPathways associated with PRKAA2 elisa kit
 Reference Product  PubMed Publications
 PRKAB1 elisa kit  >18 publications with PRKAA2 and PRKAB1
 PRKAG1 elisa kit  >17 publications with PRKAA2 and PRKAG1
 STK11 elisa kit  >12 publications with PRKAA2 and STK11
 PRKAG2 elisa kit  >8 publications with PRKAA2 and PRKAG2
 TSC2 elisa kit  >2 publications with PRKAA2 and TSC2
 FOXO3 elisa kit  >1 publications with PRKAA2 and FOXO3
 ACACB elisa kit  >1 publications with PRKAA2 and ACACB
 ACACA elisa kit  >1 publications with PRKAA2 and ACACA
 RPTOR elisa kit  >1 publications with PRKAA2 and RPTOR
 Products by Pathway  Pathway Diagram
 AMPK Inhibits ChREBP Transcriptional Activation Activity Pathway antibodies  AMPK Inhibits ChREBP Transcriptional Activation Activity Pathway Diagram
 AMPK Signaling Pathway antibodies  AMPK Signaling Pathway Diagram
 AMPK Signaling Pathway antibodies  AMPK Signaling Pathway Diagram
 AMPK Signaling Pathway antibodies  AMPK Signaling Pathway Diagram
 Activation Of PPARGC1A (PGC-1alpha) By Phosphorylation Pathway antibodies  Activation Of PPARGC1A (PGC-1alpha) By Phosphorylation Pathway Diagram
 Adipocytokine Signaling Pathway antibodies  Adipocytokine Signaling Pathway Diagram
 Adipocytokine Signaling Pathway antibodies  Adipocytokine Signaling Pathway Diagram
 BDNF Signaling Pathway antibodies  BDNF Signaling Pathway Diagram
 Cellular Responses To Stress Pathway antibodies  Cellular Responses To Stress Pathway Diagram
 Circadian Rhythm Pathway antibodies  Circadian Rhythm Pathway Diagram
Diseases associated with PRKAA2 elisa kitOrgans/Tissues associated with PRKAA2 elisa kit
 Disease Name  Pubmed Publications
 Cardiovascular Diseases Antibodies  >12 publications with PRKAA2 and Cardiovascular Diseases
 Insulin Resistance Antibodies  >12 publications with PRKAA2 and Insulin Resistance
 Inflammation Antibodies  >9 publications with PRKAA2 and Inflammation
 Heart Diseases Antibodies  >8 publications with PRKAA2 and Heart Diseases
 Disease Models, Animal Antibodies  >7 publications with PRKAA2 and Disease Models, Animal
 Liver Diseases Antibodies  >6 publications with PRKAA2 and Liver Diseases
 Cardiomyopathies Antibodies  >5 publications with PRKAA2 and Cardiomyopathies
 Hypertrophy Antibodies  >4 publications with PRKAA2 and Hypertrophy
 Fatty Liver Antibodies  >4 publications with PRKAA2 and Fatty Liver
 Hyperglycemia Antibodies  >4 publications with PRKAA2 and Hyperglycemia
 Organ/Tissue Name  Pubmed Publications
 Muscle Antibodies  >68 publications with PRKAA2 and Muscle
 Liver Antibodies  >21 publications with PRKAA2 and Liver
 Heart Antibodies  >17 publications with PRKAA2 and Heart
 Brain Antibodies  >9 publications with PRKAA2 and Brain
 Pancreas Antibodies  >7 publications with PRKAA2 and Pancreas
 Lung Antibodies  >7 publications with PRKAA2 and Lung
 Ovary Antibodies  >4 publications with PRKAA2 and Ovary
 Embryonic Tissue Antibodies  >3 publications with PRKAA2 and Embryonic Tissue
 Intestine Antibodies  >2 publications with PRKAA2 and Intestine
 Kidney Antibodies  >2 publications with PRKAA2 and Kidney
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