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ADAM10 elisa kit :: Chicken A Disintegrin And Metalloprotease 10 ELISA Kit

Scan QR to view Datasheet Catalog #    MBS737032
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $440 +1 FREE 8GB USB
96-Strip-Wells  /  $640 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,895 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,415 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

A Disintegrin And Metalloprotease 10 (ADAM10), ELISA Kit

★Popular Item★
 Also Known As   

Chicken A Disintegrin And Metalloprotease 10 ELISA Kit

 Product Gene Name   

ADAM10 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    615590
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 Species Reactivity    Chicken
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 Specificity    This assay has high sensitivity and excellent specificity for detection of ADAM10. No significant cross-reactivity or interference between ADAM10 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between ADAM10 and all the analogues, therefore, cross reaction may still exist in some cases.
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 Samples    Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
 Assay Type    Competitive
 Sensitivity    1.0 pg/mL.
 Intended Uses    This ADAM10 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Chicken ADAM10. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
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 Preparation and Storage    Store all reagents at 2-8 degree C.
 Sample Preparation    We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of ADAM10 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for ADAM10 purchase    MBS737032 is a ready-to-use microwell, strip plate Competitive ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the A Disintegrin And Metalloprotease 10 (ADAM10) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing ADAM10, with an estimated sensitivity of 1.0 pg/mL. The ELISA analytical biochemical technique of the MBS737032 kit is based on ADAM10 antibody-ADAM10 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect ADAM10 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, ADAM10. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for ADAM10 elisa kit

   Principle of the assay: ADAM10 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-ADAM10 antibody and an ADAM10-HRP conjugate. The assay sample and buffer are incubated together with ADAM10-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ADAM10 concentration since ADAM10 from samples and ADAM10-HRP conjugate compete for the anti-ADAM10 antibody binding site. Since the number of sites is limited, as more sites are occupied by ADAM10 from the sample, fewer sites are left to bind ADAM10-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ADAM10 concentration in each sample is interpolated from this standard curve.
 Product Categories/Family for ADAM10 elisa kit    Cardiovascular
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 Typical Testing Data/Standard Curve (for reference only) of ADAM10 elisa kit    ADAM10 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS737032. Click to request current manual
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NCBI/Uniprot data below describe general gene information for ADAM10. It may not necessarily be applicable to this product.
 NCBI GI #    2393947
 NCBI GeneID    102
 NCBI Accession #    AAC51766.1 [Other Products]
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 UniProt Secondary Accession #    Q10742; Q92650 [Other Products]
 UniProt Related Accession #    O14672 [Other Products]
 Molecular Weight    84,142 Da
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 NCBI Official Full Name    ADAM10
 NCBI Official Synonym Full Names    ADAM metallopeptidase domain 10
 NCBI Official Symbol    ADAM10 [Similar Products]
 NCBI Official Synonym Symbols   
RAK; kuz; AD10; AD18; MADM; CD156c; HsT18717
[Similar Products]
 NCBI Protein Information    disintegrin and metalloproteinase domain-containing protein 10; CDw156; kuzbanian protein homolog; mammalian disintegrin-metalloprotease; a disintegrin and metalloprotease domain 10
 UniProt Protein Name    Disintegrin and metalloproteinase domain-containing protein 10
 UniProt Synonym Protein Names   
CDw156; Kuzbanian protein homolog; Mammalian disintegrin-metalloprotease; CD_antigen: CD156c
 Protein Family    Disintegrin and metalloproteinase domain-containing protein
 UniProt Gene Name    ADAM10 [Similar Products]
 UniProt Synonym Gene Names    KUZ; MADM; ADAM 10 [Similar Products]
 UniProt Entry Name    ADA10_HUMAN
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 NCBI Summary for ADAM10    Members of the ADAM family are cell surface proteins with a unique structure possessing both potential adhesion and protease domains. This gene encodes and ADAM family member that cleaves many proteins including TNF-alpha and E-cadherin. [provided by RefSeq, Jul 2008]
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 UniProt Comments for ADAM10    ADAM10: Cleaves the membrane-bound precursor of TNF-alpha at '76-Ala-|-Val-77' to its mature soluble form. Responsible for the proteolytical release of soluble JAM3 from endothelial cells surface. Responsible for the proteolytic release of several other cell-surface proteins, including heparin-binding epidermal growth- like factor, ephrin-A2 and for constitutive and regulated alpha- secretase cleavage of amyloid precursor protein (APP). Contributes to the normal cleavage of the cellular prion protein. Involved in the cleavage of the adhesion molecule L1 at the cell surface and in released membrane vesicles, suggesting a vesicle-based protease activity. Controls also the proteolytic processing of Notch and mediates lateral inhibition during neurogenesis. Responsible for the FasL ectodomain shedding and for the generation of the remnant ADAM10-processed FasL (FasL APL) transmembrane form. Also cleaves the ectodomain of the integral membrane proteins CORIN and ITM2B. May regulate the EFNA5-EPHA3 signaling.

Protein type: Protease; Vesicle; Cell surface; Motility/polarity/chemotaxis; Membrane protein, integral; Cell adhesion; EC 3.4.24.81

Chromosomal Location of Human Ortholog: 15q22

Cellular Component: Golgi apparatus; cell surface; focal adhesion; intracellular membrane-bound organelle; membrane; cytoplasm; postsynaptic density; plasma membrane; integral to membrane; Golgi-associated vesicle; nucleus

Molecular Function: integrin binding; protein binding; protein homodimerization activity; zinc ion binding; metallopeptidase activity; metalloendopeptidase activity; endopeptidase activity; SH3 domain binding; protein kinase binding; receptor binding

Biological Process: epidermal growth factor receptor signaling pathway; integrin-mediated signaling pathway; axon guidance; Notch signaling pathway; extracellular matrix organization and biogenesis; in utero embryonic development; constitutive protein ectodomain proteolysis; membrane protein ectodomain proteolysis; Notch receptor processing; negative regulation of cell adhesion; positive regulation of cell growth; protein amino acid phosphorylation; collagen catabolic process; extracellular matrix disassembly; PMA-inducible membrane protein ectodomain proteolysis; monocyte activation; cell-cell signaling; positive regulation of cell proliferation; ephrin receptor signaling pathway; positive regulation of cell migration

Disease: Reticulate Acropigmentation Of Kitamura; Alzheimer Disease 18
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 Research Articles on ADAM10    1. ADAM10 negatively regulates neuronal differentiation, possibly via its proteolytic effect on the Notch signaling during development of the spinal cord.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with ADAM10 elisa kitPathways associated with ADAM10 elisa kit
 Reference Product  PubMed Publications
 EGFR elisa kit  >35 publications with ADAM10 and EGFR
 NOTCH1 elisa kit  >17 publications with ADAM10 and NOTCH1
 CTNNB1 elisa kit  >14 publications with ADAM10 and CTNNB1
 CDH2 elisa kit  >6 publications with ADAM10 and CDH2
 NOTCH3 elisa kit  >3 publications with ADAM10 and NOTCH3
 NOTCH2 elisa kit  >3 publications with ADAM10 and NOTCH2
 DLL1 elisa kit  >3 publications with ADAM10 and DLL1
 JAG1 elisa kit  >2 publications with ADAM10 and JAG1
 JAG2 elisa kit  >1 publications with ADAM10 and JAG2
 Products by Pathway  Pathway Diagram
 Activated NOTCH1 Transmits Signal To The Nucleus Pathway antibodies  Activated NOTCH1 Transmits Signal To The Nucleus Pathway Diagram
 Alzheimer's Disease Pathway antibodies  Alzheimer's Disease Pathway Diagram
 Alzheimer's Disease Pathway antibodies  Alzheimer's Disease Pathway Diagram
 Alzheimers Disease Pathway antibodies  Alzheimers Disease Pathway Diagram
 Axon Guidance Pathway antibodies  Axon Guidance Pathway Diagram
 Collagen Degradation Pathway antibodies  Collagen Degradation Pathway Diagram
 Constitutive Signaling By NOTCH1 HD Domain Mutants Pathway antibodies  Constitutive Signaling By NOTCH1 HD Domain Mutants Pathway Diagram
 Constitutive Signaling By NOTCH1 HD+PEST Domain Mutants Pathway antibodies  Constitutive Signaling By NOTCH1 HD+PEST Domain Mutants Pathway Diagram
 Constitutive Signaling By NOTCH1 PEST Domain Mutants Pathway antibodies  Constitutive Signaling By NOTCH1 PEST Domain Mutants Pathway Diagram
 Constitutive Signaling By NOTCH1 T(7;9)(NOTCH1:M1580_K2555) Translocation Mutant Pathway antibodies  Constitutive Signaling By NOTCH1 T(7;9)(NOTCH1:M1580_K2555) Translocation Mutant Pathway Diagram
Diseases associated with ADAM10 elisa kitOrgans/Tissues associated with ADAM10 elisa kit
 Disease Name  Pubmed Publications
 Necrosis Antibodies  >204 publications with ADAM10 and Necrosis
 Brain Diseases Antibodies  >123 publications with ADAM10 and Brain Diseases
 Inflammation Antibodies  >58 publications with ADAM10 and Inflammation
 Skin Diseases Antibodies  >30 publications with ADAM10 and Skin Diseases
 Cardiovascular Diseases Antibodies  >22 publications with ADAM10 and Cardiovascular Diseases
 Neoplasm Metastasis Antibodies  >20 publications with ADAM10 and Neoplasm Metastasis
 Kidney Diseases Antibodies  >18 publications with ADAM10 and Kidney Diseases
 Gastrointestinal Diseases Antibodies  >17 publications with ADAM10 and Gastrointestinal Diseases
 Breast Neoplasms Antibodies  >17 publications with ADAM10 and Breast Neoplasms
 Lung Diseases Antibodies  >15 publications with ADAM10 and Lung Diseases
 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >150 publications with ADAM10 and Brain
 Kidney Antibodies  >39 publications with ADAM10 and Kidney
 Embryonic Tissue Antibodies  >36 publications with ADAM10 and Embryonic Tissue
 Bone Antibodies  >25 publications with ADAM10 and Bone
 Skin Antibodies  >23 publications with ADAM10 and Skin
 Lung Antibodies  >20 publications with ADAM10 and Lung
 Liver Antibodies  >20 publications with ADAM10 and Liver
 Intestine Antibodies  >9 publications with ADAM10 and Intestine
 Connective Tissue Antibodies  >7 publications with ADAM10 and Connective Tissue
 Placenta Antibodies  >7 publications with ADAM10 and Placenta
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