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ALT elisa kit :: Chicken Alanine Aminotransferase (ALT) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS266858
Typical Testing Data/Standard Curve
Unit / Price
48-Strip-Wells  /  $315 +1 FREE 8GB USB
96-Strip-Wells  /  $490 +1 FREE 8GB USB
5x96-Strip-Wells  /  $1,825 +2 FREE 8GB USB
10x96-Strip-Wells  /  $3,425 +4 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Alanine Aminotransferase (ALT), ELISA Kit

 Also Known As   

Chicken Alanine Aminotransferase (ALT) ELISA Kit

 Product Gene Name   

ALT elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Manual Insert    Download PDF Manual View PDF Manual
 OMIM    138200
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 Species Reactivity    Chicken
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 Specificity    No cross-reaction with other factors.
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 Samples    Chicken serum, plasma or cell culture supernatant and organizations in the natural and recombinant ALT concentration.
 Assay Type    Sandwich
 Detection Range    200 U/L-3.12 U/L
 Sensitivity    0.6 U/L.
 Intra-assay Precision    <= 8%
 Inter-assay Precision    <= 12%
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 Preparation and Storage    Store all reagents at 2-8 degree C.
 Product Note    Our ELISA assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay.
 Other Notes    Small volumes of ALT elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for ALT purchase    MBS266858 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Alanine Aminotransferase (ALT) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 200 U/L-3.12 U/L in biological research samples containing ALT, with an estimated sensitivity of 0.6 U/L. The ELISA analytical biochemical technique of the MBS266858 kit is based on ALT antibody-ALT antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect ALT antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, ALT. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Chicken serum, plasma or cell culture supernatant and organizations in the natural and recombinant ALT concentration. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Product Description specifically for ALT elisa kit

   Principle of the Assay||This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Chicken ALT monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.
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 Typical Testing Data/Standard Curve of ALT elisa kit    ALT elisa kit Typical Testing Data/Standard Curve image
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Sample Manual Insert of MBS266858
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NCBI/Uniprot data below describe general gene information for ALT. It may not necessarily be applicable to this product.
 NCBI GI #    1507680
 NCBI GeneID    2875
 NCBI Accession #    BAA01186.1 [Other Products]
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 UniProt Secondary Accession #    P78398; Q93076; B0YJ18; D3DWM7 [Other Products]
 UniProt Related Accession #    P24298 [Other Products]
 Molecular Weight    54,637 Da
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 NCBI Official Full Name    alanine aminotransferase, partial
 NCBI Official Synonym Full Names    glutamic-pyruvate transaminase (alanine aminotransferase)
 NCBI Official Symbol    GPT [Similar Products]
 NCBI Official Synonym Symbols   
AAT1; ALT1; GPT1
[Similar Products]
 NCBI Protein Information    alanine aminotransferase 1; GPT 1; glutamic-alanine transaminase 1; glutamic--alanine transaminase 1; glutamic--pyruvic transaminase 1; glutamate pyruvate transaminase 1
 UniProt Protein Name    Alanine aminotransferase 1
 UniProt Synonym Protein Names   
Glutamate pyruvate transaminase 1; GPT 1; Glutamic--alanine transaminase 1; Glutamic--pyruvic transaminase 1
 Protein Family    NAD--protein ADP-ribosyltransferase
 UniProt Gene Name    GPT [Similar Products]
 UniProt Synonym Gene Names    AAT1; GPT1; ALT1; GPT 1 [Similar Products]
 UniProt Entry Name    ALAT1_HUMAN
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 NCBI Summary for ALT    This gene encodes cytosolic alanine aminotransaminase 1 (ALT1); also known as glutamate-pyruvate transaminase 1. This enzyme catalyzes the reversible transamination between alanine and 2-oxoglutarate to generate pyruvate and glutamate and, therefore, plays a key role in the intermediary metabolism of glucose and amino acids. Serum activity levels of this enzyme are routinely used as a biomarker of liver injury caused by drug toxicity, infection, alcohol, and steatosis. A related gene on chromosome 16 encodes a putative mitochondrial alanine aminotransaminase.[provided by RefSeq, Nov 2009]
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 UniProt Comments for ALT    GPT: Catalyzes the reversible transamination between alanine and 2-oxoglutarate to form pyruvate and glutamate. Participates in cellular nitrogen metabolism and also in liver gluconeogenesis starting with precursors transported from skeletal muscles. Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family. Alanine aminotransferase subfamily.

Protein type: EC 2.6.1.2; Transferase; Amino Acid Metabolism - alanine, aspartate and glutamate

Chromosomal Location of Human Ortholog: 8q24.3

Cellular Component: cytosol

Molecular Function: alanine transaminase activity; pyridoxal phosphate binding

Biological Process: amino acid biosynthetic process; L-alanine catabolic process
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 Research Articles on ALT    1. A new model that combines ALT, platelets, CK-18 fragments, and triglycerides was established by logistic regression among nonalcoholic fatty liver disease patients.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with ALT elisa kitPathways associated with ALT elisa kit
 Reference Product  PubMed Publications
 PGP elisa kit  >19 publications with ALT and PGP
 GOT2 elisa kit  >4 publications with ALT and GOT2
 LDHB elisa kit  >1 publications with ALT and LDHB
 Products by Pathway  Pathway Diagram
 2-Oxocarboxylic Acid Metabolism Pathway antibodies  2-Oxocarboxylic Acid Metabolism Pathway Diagram
 2-Oxocarboxylic Acid Metabolism Pathway antibodies  2-Oxocarboxylic Acid Metabolism Pathway Diagram
 Alanine And Aspartate Metabolism Pathway antibodies  Alanine And Aspartate Metabolism Pathway Diagram
 Alanine, Aspartate And Glutamate Metabolism Pathway antibodies  Alanine, Aspartate And Glutamate Metabolism Pathway Diagram
 Alanine, Aspartate And Glutamate Metabolism Pathway antibodies  Alanine, Aspartate And Glutamate Metabolism Pathway Diagram
 Amino Acid Synthesis And Interconversion (transamination) Pathway antibodies  Amino Acid Synthesis And Interconversion (transamination) Pathway Diagram
 Biosynthesis Of Amino Acids Pathway antibodies  Biosynthesis Of Amino Acids Pathway Diagram
 Biosynthesis Of Amino Acids Pathway antibodies  Biosynthesis Of Amino Acids Pathway Diagram
 Metabolism Pathway antibodies  Metabolism Pathway Diagram
 Metabolism Of Amino Acids And Derivatives Pathway antibodies  Metabolism Of Amino Acids And Derivatives Pathway Diagram
Diseases associated with ALT elisa kitOrgans/Tissues associated with ALT elisa kit
 Disease Name  Pubmed Publications
 Liver Diseases Antibodies  >899 publications with ALT and Liver Diseases
 Drug Toxicity Antibodies  >606 publications with ALT and Drug Toxicity
 Neoplasms Antibodies  >455 publications with ALT and Neoplasms
 Poisoning Antibodies  >377 publications with ALT and Poisoning
 Drug-Induced Liver Injury Antibodies  >339 publications with ALT and Drug-Induced Liver Injury
 Hepatitis Antibodies  >333 publications with ALT and Hepatitis
 Necrosis Antibodies  >250 publications with ALT and Necrosis
 Cardiovascular Diseases Antibodies  >208 publications with ALT and Cardiovascular Diseases
 Nervous System Diseases Antibodies  >199 publications with ALT and Nervous System Diseases
 Liver Neoplasms Antibodies  >163 publications with ALT and Liver Neoplasms
 Organ/Tissue Name  Pubmed Publications
 Liver Antibodies  >1699 publications with ALT and Liver
 Kidney Antibodies  >327 publications with ALT and Kidney
 Heart Antibodies  >178 publications with ALT and Heart
 Lung Antibodies  >158 publications with ALT and Lung
 Brain Antibodies  >144 publications with ALT and Brain
 Skin Antibodies  >125 publications with ALT and Skin
 Intestine Antibodies  >82 publications with ALT and Intestine
 Mixed Antibodies  >73 publications with ALT and Mixed
 Stomach Antibodies  >57 publications with ALT and Stomach
 Pancreas Antibodies  >39 publications with ALT and Pancreas
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