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ALPI elisa kit :: Chicken Alkaline Phosphatase, Intestinal ELISA Kit

Scan QR to view Datasheet Catalog #    MBS754592
Typical Standard Curve/Testing Data
Unit / Price
48-Strip-Wells  /  $470 +1 FREE 8GB USB
96-Strip-Wells  /  $675 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,925 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,490 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Alkaline Phosphatase, Intestinal (ALPI), ELISA Kit

★Popular Item★
 Also Known As   

Chicken Alkaline Phosphatase, Intestinal ELISA Kit

 Product Gene Name   

ALPI elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    171740
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 Species Reactivity    Chicken
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 Specificity    This assay has high sensitivity and excellent specificity for detection of iALP. No significant cross-reactivity or interference between iALP and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between iALP and all the analogues, therefore, cross reaction may still exist in some cases.
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 Samples    Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
 Assay Type    Quantitative Competitive
 Sensitivity    0.1 ng/mL.
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 Preparation and Storage    Store all reagents at 2-8 degree C.
 Sample Preparation    We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of ALPI elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for ALPI purchase    MBS754592 is a ready-to-use microwell, strip plate Quantitative Competitive ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Alkaline Phosphatase, Intestinal (ALPI) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing ALPI, with an estimated sensitivity of 0.1 ng/mL. The ELISA analytical biochemical technique of the MBS754592 kit is based on ALPI antibody-ALPI antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect ALPI antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, ALPI. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, cell culture supernatants, body fluid and tissue homogenate. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Product Description specifically for ALPI elisa kit

   Intended Uses: This iALP ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Chicken iALP. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Principle of the Assay: iALP ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-iALP antibody and an iALP-HRP conjugate. The assay sample and buffer are incubated together with iALP-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the iALP concentration since iALP from samples and iALP-HRP conjugate compete for the anti-iALP antibody binding site. Since the number of sites is limited, as more sites are occupied by iALP from the sample, fewer sites are left to bind iALP-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The iALP concentration in each sample is interpolated from this standard curve.
 Product Categories/Family for ALPI elisa kit    Cancer
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 Typical Standard Curve/Testing Data of ALPI elisa kit    ALPI elisa kit Typical Standard Curve/Testing Data image
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Sample Manual Insert of MBS754592. Click to request current manual
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NCBI/Uniprot data below describe general gene information for ALPI. It may not necessarily be applicable to this product.
 NCBI GI #    124376142
 NCBI GeneID    248
 NCBI Accession #    AAI32679.1 [Other Products]
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 UniProt Secondary Accession #    Q53S80; Q9UBV5; Q9UCL2; B2R7Y4 [Other Products]
 UniProt Related Accession #    P09923 [Other Products]
 Molecular Weight    56,812 Da
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 NCBI Official Full Name    Alkaline phosphatase, intestinal
 NCBI Official Synonym Full Names    alkaline phosphatase, intestinal
 NCBI Official Symbol    ALPI [Similar Products]
 NCBI Official Synonym Symbols   
IAP
[Similar Products]
 NCBI Protein Information    intestinal-type alkaline phosphatase; Kasahara isozyme; glycerophosphatase; alkaline phosphomonoesterase; intestinal alkaline phosphatase
 UniProt Protein Name    Intestinal-type alkaline phosphatase
 Protein Family    Intestinal-type alkaline phosphatase
 UniProt Gene Name    ALPI [Similar Products]
 UniProt Synonym Gene Names    IAP; Intestinal alkaline phosphatase [Similar Products]
 UniProt Entry Name    PPBI_HUMAN
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 NCBI Summary for ALPI    There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The intestinal alkaline phosphatase gene encodes a digestive brush-border enzyme. This enzyme is upregulated during small intestinal epithelial cell differentiation. [provided by RefSeq, Jul 2008]
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 UniProt Comments for ALPI    ALPI: intestinal alkaline phosphatase is one of four related alkaline phosphatases. The exact physiological function of alkaline phosphatases is not known. This protein is a GPI-anchored membrane bound glycosylated enzyme that is localized to fetal and adult intestines.

Protein type: Membrane protein, GPI anchor; Phosphatase; EC 3.1.3.1; Cofactor and Vitamin Metabolism - folate biosynthesis; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 2q37.1

Cellular Component: integral to membrane; plasma membrane

Molecular Function: protease binding; zinc ion binding; alkaline phosphatase activity; magnesium ion binding

Biological Process: dephosphorylation
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 Research Articles on ALPI    1. Report lowered intestinal alkaline phosphatase in the colonic mucosa of children with inflammatory bowel disease.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with ALPI elisa kitPathways associated with ALPI elisa kit
 Reference Product  PubMed Publications
 INHA elisa kit  >2 publications with ALPI and INHA
 PAX3 elisa kit  >2 publications with ALPI and PAX3
 KLF4 elisa kit  >2 publications with ALPI and KLF4
 COL4A3 elisa kit  >1 publications with ALPI and COL4A3
 CHRND elisa kit  >1 publications with ALPI and CHRND
 Products by Pathway  Pathway Diagram
 Folate Biosynthesis Pathway antibodies  Folate Biosynthesis Pathway Diagram
 Folate Biosynthesis Pathway antibodies  Folate Biosynthesis Pathway Diagram
 Metabolic Pathways antibodies  Metabolic Pathways Diagram
Diseases associated with ALPI elisa kitOrgans/Tissues associated with ALPI elisa kit
 Disease Name  Pubmed Publications
 Lung Diseases Antibodies  >9 publications with ALPI and Lung Diseases
 Kidney Diseases Antibodies  >8 publications with ALPI and Kidney Diseases
 Cardiovascular Diseases Antibodies  >7 publications with ALPI and Cardiovascular Diseases
 Anemia Antibodies  >7 publications with ALPI and Anemia
 Inflammation Antibodies  >7 publications with ALPI and Inflammation
 Colitis Antibodies  >5 publications with ALPI and Colitis
 Necrosis Antibodies  >5 publications with ALPI and Necrosis
 Colonic Neoplasms Antibodies  >4 publications with ALPI and Colonic Neoplasms
 Hypertriglyceridemia Antibodies  >3 publications with ALPI and Hypertriglyceridemia
 Fatty Liver Antibodies  >3 publications with ALPI and Fatty Liver
 Organ/Tissue Name  Pubmed Publications
 Intestine Antibodies  >42 publications with ALPI and Intestine
 Heart Antibodies  >9 publications with ALPI and Heart
 Amniotic Fluid Antibodies  >2 publications with ALPI and Amniotic Fluid
 Placenta Antibodies  >2 publications with ALPI and Placenta
 Esophagus Antibodies  >1 publications with ALPI and Esophagus
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