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AREG elisa kit :: Chicken Amphiregulin ELISA Kit

Scan QR to view Datasheet Catalog #    MBS740233
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $455 +1 FREE 8GB USB
96-Strip-Wells  /  $660 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,925 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,490 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Amphiregulin (AREG), ELISA Kit

 Also Known As   

Chicken Amphiregulin ELISA Kit

 Product Gene Name   

AREG elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    104640
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 Species Reactivity    Chicken
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 Samples    Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
 Assay Type    Competitive
 Sensitivity    1.0 pg/mL.
 Intended Uses    This AREG ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Chicken AREG. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
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 Preparation and Storage    Store all reagents at 2-8 degree C.
 Sample Preparation    We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of AREG elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for AREG purchase    MBS740233 is a ready-to-use microwell, strip plate Competitive ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Amphiregulin (AREG) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing AREG, with an estimated sensitivity of 1.0 pg/mL. The ELISA analytical biochemical technique of the MBS740233 kit is based on AREG antibody-AREG antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect AREG antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, AREG. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for AREG elisa kit

   Principle of the Assay: AREG ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-AREG antibody and an AREG-HRP conjugate. The assay sample and buffer are incubated together with AREG-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the AREG concentration since AREG from samples and AREG-HRP conjugate compete for the anti-AREG antibody binding site. Since the number of sites is limited, as more sites are occupied by AREG from the sample, fewer sites are left to bind AREG-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The AREG concentration in each sample is interpolated from this standard curve.
 Product Categories/Family for AREG elisa kit    Cardiovascular
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 Typical Testing Data/Standard Curve (for reference only) of AREG elisa kit    AREG elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS740233. Click to request current manual
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NCBI/Uniprot data below describe general gene information for AREG. It may not necessarily be applicable to this product.
 NCBI GI #    179040
 NCBI GeneID    374
 NCBI Accession #    AAA51781.1 [Other Products]
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 UniProt Secondary Accession #    Q5U026 [Other Products]
 UniProt Related Accession #    P15514 [Other Products]
 Molecular Weight    27,895 Da
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 NCBI Official Full Name    amphiregulin
 NCBI Official Synonym Full Names    amphiregulin
 NCBI Official Symbol    AREG [Similar Products]
 NCBI Official Synonym Symbols   
AR; SDGF; AREGB; CRDGF
[Similar Products]
 NCBI Protein Information    amphiregulin; amphiregulin B; schwannoma-derived growth factor; colorectum cell-derived growth factor
 UniProt Protein Name    Amphiregulin
 UniProt Synonym Protein Names   
Colorectum cell-derived growth factor; CRDGF
 Protein Family    Amphiregulin
 UniProt Gene Name    AREG [Similar Products]
 UniProt Synonym Gene Names    SDGF
AREGB; AR; CRDGF
[Similar Products]
 UniProt Entry Name    AREG_HUMAN
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 NCBI Summary for AREG    The protein encoded by this gene is a member of the epidermal growth factor family. It is an autocrine growth factor as well as a mitogen for astrocytes, Schwann cells and fibroblasts. It is related to epidermal growth factor (EGF) and transforming growth factor alpha (TGF-alpha). The protein interacts with the EGF/TGF-alpha receptor to promote the growth of normal epithelial cells, and it inhibits the growth of certain aggressive carcinoma cell lines. It also functions in mammary gland, oocyte and bone tissue development. This gene is associated with a psoriasis-like skin phenotype, and is also associated with other pathological disorders, including various types of cancers and inflammatory conditions. [provided by RefSeq, Apr 2014]
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 UniProt Comments for AREG    AREG: Ligand of the EGF receptor/EGFR. Autocrine growth factor as well as a mitogen for a broad range of target cells including astrocytes, Schwann cells and fibroblasts. Belongs to the amphiregulin family.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 4q13.3

Cellular Component: extracellular space; cell surface; cytoplasm; integral to membrane; nucleus

Molecular Function: protein binding; growth factor activity; cytokine activity; epidermal growth factor receptor binding

Biological Process: epidermal growth factor receptor signaling pathway; response to peptide hormone stimulus; response to cAMP; response to glucocorticoid stimulus; response to estradiol stimulus; glial cell proliferation; G-protein coupled receptor protein signaling pathway; cell proliferation; response to hydrogen peroxide; cell-cell signaling; positive regulation of cell proliferation; negative regulation of osteoblast differentiation; neurite development; positive regulation of DNA replication; positive regulation of phosphorylation
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 Research Articles on AREG    1. aberrantly activated AREG-EGFR signaling is required for CRTC1-MAML2-positive MEC cell growth and survival, suggesting that EGFR-targeted therapies will benefit patients with advanced, unresectable CRTC1-MAML2-positive MEC.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with AREG elisa kitPathways associated with AREG elisa kit
 Reference Product  PubMed Publications
 EGFR elisa kit  >105 publications with AREG and EGFR
 PTGS2 elisa kit  >32 publications with AREG and PTGS2
 ERBB2 elisa kit  >16 publications with AREG and ERBB2
 PGR elisa kit  >13 publications with AREG and PGR
 ERBB3 elisa kit  >6 publications with AREG and ERBB3
 CYP11A1 elisa kit  >3 publications with AREG and CYP11A1
 ATF3 elisa kit  >2 publications with AREG and ATF3
 PTHLH elisa kit  >1 publications with AREG and PTHLH
 AKT1 elisa kit  >1 publications with AREG and AKT1
 Products by Pathway  Pathway Diagram
 ErbB Receptor Signaling Network Pathway antibodies  ErbB Receptor Signaling Network Pathway Diagram
 ErbB Signaling Pathway antibodies  ErbB Signaling Pathway Diagram
 ErbB Signaling Pathway antibodies  ErbB Signaling Pathway Diagram
 ErbB Signaling Pathway antibodies  ErbB Signaling Pathway Diagram
 FSH Signaling Pathway antibodies  FSH Signaling Pathway Diagram
 Hippo Signaling Pathway antibodies  Hippo Signaling Pathway Diagram
 Hippo Signaling Pathway antibodies  Hippo Signaling Pathway Diagram
Diseases associated with AREG elisa kitOrgans/Tissues associated with AREG elisa kit
 Disease Name  Pubmed Publications
 Breast Neoplasms Antibodies  >28 publications with AREG and Breast Neoplasms
 Necrosis Antibodies  >20 publications with AREG and Necrosis
 Adenocarcinoma Antibodies  >18 publications with AREG and Adenocarcinoma
 Inflammation Antibodies  >18 publications with AREG and Inflammation
 Neoplasms, Experimental Antibodies  >13 publications with AREG and Neoplasms, Experimental
 Neoplasm Metastasis Antibodies  >11 publications with AREG and Neoplasm Metastasis
 Liver Diseases Antibodies  >10 publications with AREG and Liver Diseases
 Lung Diseases Antibodies  >9 publications with AREG and Lung Diseases
 Liver Neoplasms Antibodies  >9 publications with AREG and Liver Neoplasms
 Lung Neoplasms Antibodies  >9 publications with AREG and Lung Neoplasms
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >26 publications with AREG and Blood
 Lung Antibodies  >24 publications with AREG and Lung
 Mammary Gland Antibodies  >21 publications with AREG and Mammary Gland
 Skin Antibodies  >13 publications with AREG and Skin
 Liver Antibodies  >13 publications with AREG and Liver
 Prostate Antibodies  >9 publications with AREG and Prostate
 Vascular Antibodies  >8 publications with AREG and Vascular
 Intestine Antibodies  >6 publications with AREG and Intestine
 Bone Marrow Antibodies  >6 publications with AREG and Bone Marrow
 Uterus Antibodies  >5 publications with AREG and Uterus
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