Antibody Protein ELISA Kit from MyBioSource header Telephone 1.858.633.0165          
1.855.MyBioSource
Fax 1.858.633.0166    Email sales@mybiosource.com 
tel/fax
menu1 Home Products Ordering Company Help menu7
bottom
   MyBioSource rightarrow ELISA Kit rightarrow C4b rightarrow Chicken C4b  rightarrow LOG IN  rightarrow MY ACCOUNT  rightarrow CART CONTENTS  rightarrow CHECKOUT 
horiz bar
MyBioSource Menu separator
separator
ELISA Kit ELISA Kit
CLIA Kit CLIA Kit
PCR Kit PCR Kit
Monoclonal Antibody Monoclonal Antibody
Polyclonal Antibody Polyclonal Antibody
Secondary Antibody Secondary Antibody
Antigen Antigen
Biochemical Biochemical
cDNA Clone cDNA Clone
siRNA siRNA
Peptide Peptide
Recombinant/Purified Protein Rec./Purified Protein

Custom ELISA Kit Custom ELISA Kit
Custom Protein Custom Protein
Custom Antibody Custom Antibody
Antibody Matched Pairs Antibody Matched Pairs
Antibody & Corresponding Blocking Peptide Pairs Antibody Peptide Pairs
Phospho-Specific Antibodies Phospho Antibodies
Products by Disease Products by Disease
Products by Pathway Products by Pathway
Products by Tissue Products by Tissue

arrow Advanced Search
arrow Submit Technical Q&A
arrow International Distributors
arrow Contact Us
separator
Our Best Sellers moreseparator
separator
 • COP9 signalosome complex subunit 7a (COPS7A) Antibody
 • CytoKeratin 17 (CK 17) ELISA Kit
 • Pigment Epithelium Derived Factor (PEDF) ELISA Kit
 • TNFRSF10B Antibody
 • Epiplakin (EPPK1) Antibody
 • tumor protein p53 inducible nuclear protein 1 (TP53INP1) ELISA Kit
 • Bacillus anthracis Protective Antigen (Anthrax) Antibody
 • Alternaria alternata Major allergen Alt a 1 (ALTA1) Antibody
 • Connexin 26 (CX26) ELISA Kit
 • Collagenase 3 (MMP13) Antibody
 • NGFR Antibody
 • Fibroblast Growth Factor 1, Acidic (FGF1) ELISA Kit
 • beta Galactosidase (GLB1) Antibody
 • ATG7 Antibody
 downarrow more ...
separator
separator
DatasheetFull DatasheetPrinter Friendly DatasheetPrint This DatasheetAdd to Compare ListHave Questions? Ask UsRequest for a Quotation today

C4b elisa kit :: Chicken C4b ELISA Kit

Scan QR to view Datasheet Catalog #    MBS2502718
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $450 +1 FREE 8GB USB
96-Strip-Wells  /  $535 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,255 +2 FREE 8GB USB
10x96-Strip-Wells  /  $3,975 +4 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

C4b, ELISA Kit

 Also Known As   

Chicken C4b (Complement Component 4b) ELISA Kit

 Product Gene Name   

C4b elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
Table BarTOPTable Bar
  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    120820
 3D Structure    ModBase 3D Structure for P0C0L5
Section Bar
 Species Reactivity    Chicken
Section Bar
 Specificity    This kit recognizes natural and recombinant Chicken C4b. No significant cross-reactivity or interference between Chicken C4b and analogues was observed.
Section Bar
 Samples    Serum, Plasma, Biological Fluids
 Assay Type    Sandwich
 Detection Range    0.234-15ng/mL
 Sensitivity    Min: 0.14ng/mL; Max: 15ng/mL
Section Bar
 Preparation and Storage    Store at 4 degree C.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of C4b elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for C4b purchase    MBS2502718 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the C4b, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 0.234-15ng/mL in biological research samples containing C4b, with an estimated sensitivity of Min: 0.14ng/mL; Max: 15ng/mL. The ELISA analytical biochemical technique of the MBS2502718 kit is based on C4b antibody-C4b antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect C4b antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, C4b. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, Plasma, Biological Fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Table BarTOPTable Bar
 

Related Product Information for C4b elisa kit

   Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Chicken C4b concentrations in serum, plasma and other biological fluids.

Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to C4b. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for C4b and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain C4b, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of C4b. You can calculate the concentration of C4b in the samples by comparing the OD of the samples to the standard curve.
Section Bar
 Typical Testing Data/Standard Curve (for reference only) of C4b elisa kit    C4b elisa kit Typical Testing Data/Standard Curve (for reference only) image
Table BarTOPTable Bar
Sample Manual Insert of MBS2502718. Click to request current manual
Table BarTOPTable Bar
NCBI/Uniprot data below describe general gene information for C4b. It may not necessarily be applicable to this product.
 NCBI GI #    178557739
 NCBI GeneID    721
 NCBI Accession #    NP_001002029.3 [Other Products]
 NCBI GenBank Nucleotide #    NM_001002029.3 [Other Products]
Section Bar
 UniProt Primary Accession #    P0C0L5 [Other Products]
 UniProt Secondary Accession #    P01028; P78445; Q13160; Q13906; Q14033; Q14835; Q6U2E9; Q6U2G1; Q6U2I5; Q6U2L1; A2BHY4 [Other Products]
 UniProt Related Accession #    P0C0L5 [Other Products]
 Molecular Weight    192,751 Da
Table BarTOPTable Bar
 NCBI Official Full Name    complement C4-B preproprotein
 NCBI Official Synonym Full Names    complement component 4B (Chido blood group)
 NCBI Official Symbol    C4B [Similar Products]
 NCBI Official Synonym Symbols   
CH; C4F; CO4; C4B1; C4B2; C4B3; C4B5; C4BD; C4B12; C4B_2; CPAMD3
[Similar Products]
 NCBI Protein Information    complement C4-B; C3 and PZP-like alpha-2-macroglobulin domain-containing protein 3; Chido form of C4; basic complement C4; complement C4B1a
 UniProt Protein Name    Complement C4-B
 UniProt Synonym Protein Names   
Basic complement C4; C3 and PZP-like alpha-2-macroglobulin domain-containing protein 3Cleaved into the following 6 chains:Complement C4 beta chain; Complement C4-B alpha chain; C4a anaphylatoxin; C4b-B; C4d-B; Complement C4 gamma chain
 Protein Family    Complement C4
 UniProt Gene Name    C4B [Similar Products]
 UniProt Synonym Gene Names    CO4; CPAMD3 [Similar Products]
 UniProt Entry Name    CO4B_HUMAN
Section Bar
 NCBI Summary for C4b    This gene encodes the basic form of complement factor 4, part of the classical activation pathway. The protein is expressed as a single chain precursor which is proteolytically cleaved into a trimer of alpha, beta, and gamma chains prior to secretion. The trimer provides a surface for interaction between the antigen-antibody complex and other complement components. The alpha chain may be cleaved to release C4 anaphylatoxin, a mediator of local inflammation. Deficiency of this protein is associated with systemic lupus erythematosus. This gene localizes to the major histocompatibility complex (MHC) class III region on chromosome 6. Varying haplotypes of this gene cluster exist, such that individuals may have 1, 2, or 3 copies of this gene. In addition, this gene exists as a long form and a short form due to the presence or absence of a 6.4 kb endogenous HERV-K retrovirus in intron 9. [provided by RefSeq, Jul 2008]
Section Bar
 UniProt Comments for C4b    C4B: C4 plays a central role in the activation of the classical pathway of the complement system. It is processed by activated C1 which removes from the alpha chain the C4a anaphylatoxin. The remaining alpha chain fragment C4b is the major activation product and is an essential subunit of the C3 convertase (C4b2a) and the C5 convertase (C3bC4b2a) enzymes of the classical complement pathway. Defects in C4B are a cause of susceptibility to systemic lupus erythematosus (SLE). A chronic, inflammatory and often febrile multisystemic disorder of connective tissue. It affects principally the skin, joints, kidneys and serosal membranes. It is thought to represent a failure of the regulatory mechanisms of the autoimmune system. Interindividual copy- number variation (CNV) of complement component C4 and associated polymorphisms result in different susceptibilities to SLE. The risk of SLE susceptibility has been shown to be significantly increased among subjects with only two copies of total C4. A high copy number is a protective factor against SLE. Defects in C4B are the cause of complement component 4B deficiency (C4BD). A rare defect of the complement classical pathway associated with the development of autoimmune disorders, mainly systemic lupus with or without associated glomerulonephritis.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 6p21.3

Cellular Component: extracellular space; extracellular region; plasma membrane

Molecular Function: complement binding; endopeptidase inhibitor activity; carbohydrate binding

Biological Process: detection of molecule of bacterial origin; regulation of complement activation; innate immune response; opsonization; inflammatory response; complement activation, classical pathway; complement activation

Disease: Complement Component 4b Deficiency
Table BarTOPTable Bar
 Research Articles on C4b    1. our study indicates that Finnish NTM patients had significantly more often C4 deficiencies than the healthy control subjects.
Table BarTOPTable Bar
 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Table BarTOPTable Bar
Products associated with C4b elisa kitPathways associated with C4b elisa kit
 Reference Product  PubMed Publications
 C1S elisa kit  >65 publications with C4b and C1S
 TNXA elisa kit  >15 publications with C4b and TNXA
 CYP21A2 elisa kit  >12 publications with C4b and CYP21A2
 HLA-ABC elisa kit  >3 publications with C4b and HLA-ABC
 C5AR1 elisa kit  >1 publications with C4b and C5AR1
 Products by Pathway  Pathway Diagram
 Activation Of C3 And C5 Pathway antibodies  Activation Of C3 And C5 Pathway Diagram
 Complement Activation, Classical Pathway antibodies  Complement Activation, Classical Pathway Diagram
 Complement And Coagulation Cascades Pathway antibodies  Complement And Coagulation Cascades Pathway Diagram
 Complement And Coagulation Cascades Pathway antibodies  Complement And Coagulation Cascades Pathway Diagram
 Complement Cascade Pathway antibodies  Complement Cascade Pathway Diagram
 Immune System Pathway antibodies  Immune System Pathway Diagram
 Initial Triggering Of Complement Pathway antibodies  Initial Triggering Of Complement Pathway Diagram
 Innate Immune System Pathway antibodies  Innate Immune System Pathway Diagram
 Pertussis Pathway antibodies  Pertussis Pathway Diagram
 Pertussis Pathway antibodies  Pertussis Pathway Diagram
Organs/Tissues associated with C4b elisa kit
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >1167 publications with C4b and Blood
 Kidney Antibodies  >389 publications with C4b and Kidney
 Liver Antibodies  >149 publications with C4b and Liver
 Heart Antibodies  >101 publications with C4b and Heart
 Lung Antibodies  >74 publications with C4b and Lung
 Muscle Antibodies  >49 publications with C4b and Muscle
 Brain Antibodies  >49 publications with C4b and Brain
 Bone Antibodies  >25 publications with C4b and Bone
 Spleen Antibodies  >24 publications with C4b and Spleen
 Nerve Antibodies  >18 publications with C4b and Nerve
Table BarTOPTable Bar
horiz bar
 SSL   Follow us on Facebook Follow us onTwitter Follow us on Google Plus Connect us on LinkedIn Subscribe to our RSS Feed for latest products and special promotions