|
| Product Name
|
|
Calbindin (CALB1), ELISA Kit
|
| Also Known As
|
|
Chicken Calbindin ELISA Kit
|
| Product Synonym Names
|
|
Calbindin; Calbindin D28; D-28K; Vitamin D-dependent calcium-binding protein, avian-type; CALB1
|
| Product Gene Name
|
|
|
[Similar Products]
|
| Research Use Only
|
|
For Research Use Only. Not for use in diagnostic procedures.
|
 TOP
|
| Sample Manual Insert
|
|
 
|
| Request for Current Manual Insert
|
|
 |
|
| 3D Structure
|
|
ModBase 3D Structure for P04354
|
| Species Reactivity
|
|
Chicken
|
|
| Specificity
|
|
This assay recognizes recombinant and natural chicken Calbindin. No significant cross-reactivity or interference was observed.
|
|
|
| Samples
|
|
serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
|
| Assay Type
|
|
Quantitative ELISA
|
| Detection Range
|
|
0.62 ng/mL -40.0 ng/mL.
|
|
|
| Preparation and Storage
|
|
For long term storage, please store the entire kit at -20 degree C.
|
| Product Note
|
|
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
|
| Other Notes
|
|
Small volumes of CALB1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
|
| Searchable Terms for CALB1 purchase
|
|
MBS2883935 is a ready-to-use microwell, strip plate Quantitative ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Calbindin (CALB1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 0.62 ng/mL -40.0 ng/mL in biological research samples containing CALB1. The ELISA analytical biochemical technique of the MBS2883935 kit is based on CALB1 antibody-CALB1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect CALB1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, CALB1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
|
|
TOP
|
|
Related Product Information for CALB1 elisa kit
|
|
Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of chicken Calbindin concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to Calbindin. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Calbindin and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Calbindin, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of Calbindin in the samples is then determined by comparing the O.D. of the samples to the standard curve.
|
|
|
|
| Typical Testing Data/Standard Curve (for reference only) of CALB1 elisa kit
|
|
|
|
TOP
|
|
| Sample Manual Insert of MBS2883935. Click to request current manual
|
|
|
|
TOP
|
|
| NCBI/Uniprot data below describe general gene information for CALB1. It may not necessarily be applicable to this product. |
| NCBI GI #
|
|
45382893
|
| NCBI GeneID
|
|
396519
|
| NCBI Accession #
|
|
NP_990844.1
|
[Other Products]
|
| NCBI GenBank Nucleotide #
|
|
NM_205513.1
|
[Other Products]
|
|
|
| UniProt Primary Accession #
|
|
P04354
|
[Other Products]
|
| UniProt Related Accession #
|
|
P04354
|
[Other Products]
|
| Molecular Weight
|
|
30,167 Da
|
|
TOP
|
| NCBI Official Full Name
|
|
calbindin
|
| NCBI Official Synonym Full Names
|
|
calbindin 1, 28kDa
|
| NCBI Official Symbol
|
|
CALB1
|
[Similar Products]
|
| NCBI Protein Information
|
|
calbindin
|
| UniProt Protein Name
|
|
Calbindin
|
| UniProt Synonym Protein Names
|
|
Calbindin D28; D-28K; Vitamin D-dependent calcium-binding protein, avian-type
|
| Protein Family
|
|
Calbindin
|
| UniProt Gene Name
|
|
CALB1
|
[Similar Products]
|
| UniProt Entry Name
|
|
CALB1_CHICK
|
|
|
| UniProt Comments for CALB1
|
|
CALB1: Buffers cytosolic calcium. May stimulate a membrane Ca(2+)-ATPase and a 3',5'-cyclic nucleotide phosphodiesterase
Protein type: Calcium-binding
Cellular Component: cell soma; cytosol; dendrite; neuron projection; nucleus; postsynaptic membrane; vesicle membrane
Molecular Function: calcium ion binding; vitamin D binding; zinc ion binding
Biological Process: calcium ion transport; cellular calcium ion homeostasis; cytosolic calcium ion homeostasis; locomotory behavior; long-term memory; sensory perception of pain; short-term memory
|
|
TOP
|
|
| Research Articles on CALB1
|
|
1. The vector-based transient receptor potential vanilloid 6(TRPV6)-shRNA can efficiently suppress the mRNA and protein expression of TRPV6 in chicken osteoblasts, and TRPV6 regulates the expression of calbindin-D28K during Ca2+ transport.
|
|
TOP
|
| Precautions
|
|
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
|
| Disclaimer
|
|
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
|
|
TOP
|
|
| Products associated with CALB1 elisa kit | Diseases associated with CALB1 elisa kit |
|
|
|
| Organs/Tissues associated with CALB1 elisa kit |
|
|
|
|
|
TOP
|
ELISA Kit 
