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DEFA1 elisa kit :: Chicken Defensin 1, Neutrophil ELISA Kit

Scan QR to view Datasheet Catalog #    MBS741835
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $470 +1 FREE 8GB USB
96-Strip-Wells  /  $675 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,925 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,490 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Defensin 1, Neutrophil (DEFA1), ELISA Kit

 Also Known As   

Chicken Defensin 1, Neutrophil ELISA Kit

 Product Gene Name   

DEFA1 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Manual Insert    Download PDF Manual View PDF Manual
 Chromosome Location    Chromosome: 8; NC_000008.10 (6835171..6837614, complement). Location: 8p23.1
 OMIM    125220
 3D Structure    ModBase 3D Structure for P59665
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 Species Reactivity    Chicken
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 Samples    Serum, plasma, cell culture supern atants, body fluid and tissue homogenate
 Assay Type    Competitive
 Sensitivity    1.0 pg/mL.
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 Preparation and Storage    Store all reagents at 2-8 degree C
 Sample Preparation    We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
 Product Note    Our ELISA assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay.
 Other Notes    Small volumes of DEFA1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for DEFA1 purchase    MBS741835 is a ready-to-use microwell, strip plate Competitive ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Defensin 1, Neutrophil (DEFA1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing DEFA1, with an estimated sensitivity of 1.0 pg/mL. The ELISA analytical biochemical technique of the MBS741835 kit is based on DEFA1 antibody-DEFA1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect DEFA1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, DEFA1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, cell culture supern atants, body fluid and tissue homogenate. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Product Description specifically for DEFA1 elisa kit

   For samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Intended Use This DEFA1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Chicken DEFA1. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

PRINCIPLE OF THE ASSAY DEFA1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-DEFA1 antibody and an DEFA1-HRP conjugate. The assay sample and buffer are incubated together with DEFA1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the DEFA1 concentration since DEFA1 from samples and DEFA1-HRP conjugate compete for the anti-DEFA1 antibody binding site. Since the number of sites is limited, as more sites are occupied by DEFA1 from the sample, fewer sites are left to bind DEFA1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The DEFA1 concentration in each sample is interpolated from this standard curve.
 Product Categories/Family for DEFA1 elisa kit    Immunology
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 Typical Testing Data/Standard Curve (for reference only) of DEFA1 elisa kit    DEFA1 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS741835
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NCBI/Uniprot data below describe general gene information for DEFA1. It may not necessarily be applicable to this product.
 NCBI GI #    50057842
 NCBI GeneID    1667
 NCBI Accession #    AAT68878.1 [Other Products]
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 UniProt Primary Accession #    P59665 [Other Products]
 UniProt Secondary Accession #    P11479; Q14125; Q6EZF6 [Other Products]
 UniProt Related Accession #    P59665 [Other Products]
 Molecular Weight    10,201 Da
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 NCBI Official Full Name    defensin, alpha 1, myeloid-related sequence
 NCBI Official Synonym Full Names    defensin, alpha 1
 NCBI Official Symbol    DEFA1 [Similar Products]
 NCBI Official Synonym Symbols   
HP1; MRS; DEF1; HP-1; DEFA2; HNP-1; DEFA1B
[Similar Products]
 NCBI Protein Information    neutrophil defensin 1; defensin, alpha 2; myeloid-related sequence; defensin, alpha 1, myeloid-related sequence
 UniProt Protein Name    Neutrophil defensin 1
 UniProt Synonym Protein Names   
Defensin, alpha 1; HNP-1; HP-1; HP1Cleaved into the following 2 chains:HP 1-56Neutrophil defensin 2; Alternative name(s):; HNP-2
 Protein Family    Alpha-defensin
 UniProt Gene Name    DEFA1 [Similar Products]
 UniProt Synonym Gene Names    DEF1; DEFA2; MRS; HP-1; HP1; HP-2; HP2 [Similar Products]
 UniProt Entry Name    DEF1_HUMAN
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 NCBI Summary for DEFA1    Defensins are a family of microbicidal and cytotoxic peptides thought to be involved in host defense. They are abundant in the granules of neutrophils and also found in the epithelia of mucosal surfaces such as those of the intestine, respiratory tract, urinary tract, and vagina. Members of the defensin family are highly similar in protein sequence and distinguished by a conserved cysteine motif. The protein encoded by this gene, defensin, alpha 1, is found in the microbicidal granules of neutrophils and likely plays a role in phagocyte-mediated host defense. Several alpha defensin genes are clustered on chromosome 8. This gene differs from defensin, alpha 3 by only one amino acid. This gene and the gene encoding defensin, alpha 3 are both subject to copy number variation. [provided by RefSeq, May 2010]
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 UniProt Comments for DEFA1    defensin, alpha 1: Defensin 1 and defensin 2 have antibacterial, fungicide and antiviral activities. Has antimicrobial activity against Gram- negative and Gram-positive bacteria. Defensins are thought to kill microbes by permeabilizing their plasma membrane. Belongs to the alpha-defensin family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 8p23.1

Cellular Component: extracellular matrix; extracellular space; Golgi lumen; extracellular region

Biological Process: defense response to Gram-positive bacterium; estrogen receptor signaling pathway; innate immune response in mucosa; killing of cells of another organism; antibacterial humoral response; innate immune response; immune response; chemotaxis; defense response to fungus; defense response to virus
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 Research Articles on DEFA1    1. Dimerization contributes to some but not all of the activities of HNP1.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with DEFA1 elisa kitPathways associated with DEFA1 elisa kit
 Reference Product  PubMed Publications
 DEFA3 elisa kit  >7 publications with DEFA1 and DEFA3
 Products by Pathway  Pathway Diagram
 Alpha-defensins Pathway antibodies  Alpha-defensins Pathway Diagram
 Cellular Roles Of Anthrax Toxin Pathway antibodies  Cellular Roles Of Anthrax Toxin Pathway Diagram
 Defensins Pathway antibodies  Defensins Pathway Diagram
 Immune System Pathway antibodies  Immune System Pathway Diagram
 Innate Immune System Pathway antibodies  Innate Immune System Pathway Diagram
Diseases associated with DEFA1 elisa kitOrgans/Tissues associated with DEFA1 elisa kit
 Disease Name  Pubmed Publications
 Inflammation Antibodies  >5 publications with DEFA1 and Inflammation
 Neoplasms Antibodies  >3 publications with DEFA1 and Neoplasms
 Colitis Antibodies  >2 publications with DEFA1 and Colitis
 Colorectal Neoplasms Antibodies  >1 publications with DEFA1 and Colorectal Neoplasms
 Craniofacial Abnormalities Antibodies  >1 publications with DEFA1 and Craniofacial Abnormalities
 Fibrosis Antibodies  >1 publications with DEFA1 and Fibrosis
 Dyspnea Antibodies  >1 publications with DEFA1 and Dyspnea
 Adenocarcinoma Antibodies  >1 publications with DEFA1 and Adenocarcinoma
 Neoplasm Metastasis Antibodies  >1 publications with DEFA1 and Neoplasm Metastasis
 Limb Deformities, Congenital Antibodies  >1 publications with DEFA1 and Limb Deformities, Congenital
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >13 publications with DEFA1 and Blood
 Bone Antibodies  >2 publications with DEFA1 and Bone
 Liver Antibodies  >1 publications with DEFA1 and Liver
 Bone Marrow Antibodies  >1 publications with DEFA1 and Bone Marrow
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