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| Product Name
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Defensin 1, Neutrophil (DEFA1), ELISA Kit
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| Also Known As
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Chicken Defensin 1, Neutrophil ELISA Kit
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| Product Gene Name
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[Similar Products]
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| Research Use Only
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For Research Use Only. Not for use in diagnostic procedures.
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| Sample Manual Insert
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| Request for Current Manual Insert
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| Chromosome Location
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Chromosome: 8; NC_000008.10 (6835171..6837614, complement). Location: 8p23.1
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| OMIM
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125220
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| 3D Structure
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ModBase 3D Structure for P59665
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| Species Reactivity
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Chicken
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| Samples
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Serum, plasma, cell culture supern atants, body fluid and tissue homogenate
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| Assay Type
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Competitive
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| Sensitivity
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1.0 pg/mL.
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| Preparation and Storage
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Store all reagents at 2-8 degree C
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| Sample Preparation
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We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
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| Product Note
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Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
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| Other Notes
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Small volumes of DEFA1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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| Searchable Terms for DEFA1 purchase
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MBS741835 is a ready-to-use microwell, strip plate Competitive ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Defensin 1, Neutrophil (DEFA1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing DEFA1, with an estimated sensitivity of 1.0 pg/mL. The ELISA analytical biochemical technique of the MBS741835 kit is based on DEFA1 antibody-DEFA1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect DEFA1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, DEFA1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, cell culture supern atants, body fluid and tissue homogenate. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for DEFA1 elisa kit
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For samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Intended Use This DEFA1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Chicken DEFA1. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
PRINCIPLE OF THE ASSAY DEFA1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-DEFA1 antibody and an DEFA1-HRP conjugate. The assay sample and buffer are incubated together with DEFA1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the DEFA1 concentration since DEFA1 from samples and DEFA1-HRP conjugate compete for the anti-DEFA1 antibody binding site. Since the number of sites is limited, as more sites are occupied by DEFA1 from the sample, fewer sites are left to bind DEFA1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The DEFA1 concentration in each sample is interpolated from this standard curve.
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| Product Categories/Family for DEFA1 elisa kit
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Immunology
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| Typical Testing Data/Standard Curve (for reference only) of DEFA1 elisa kit
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| Sample Manual Insert of MBS741835. Click to request current manual
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| NCBI/Uniprot data below describe general gene information for DEFA1. It may not necessarily be applicable to this product. |
| NCBI GI #
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50057842
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| NCBI GeneID
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1667
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| NCBI Accession #
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AAT68878.1
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[Other Products]
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| UniProt Primary Accession #
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P59665
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[Other Products]
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| UniProt Secondary Accession #
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P11479; Q14125; Q6EZF6
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[Other Products]
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| UniProt Related Accession #
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P59665
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[Other Products]
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| Molecular Weight
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10,201 Da
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| NCBI Official Full Name
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defensin, alpha 1, myeloid-related sequence
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| NCBI Official Synonym Full Names
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defensin, alpha 1
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| NCBI Official Symbol
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DEFA1
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[Similar Products]
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| NCBI Official Synonym Symbols
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HP1; MRS; DEF1; HP-1; DEFA2; HNP-1; DEFA1B
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[Similar Products]
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| NCBI Protein Information
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neutrophil defensin 1; defensin, alpha 2; myeloid-related sequence; defensin, alpha 1, myeloid-related sequence
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| UniProt Protein Name
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Neutrophil defensin 1
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| UniProt Synonym Protein Names
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Defensin, alpha 1; HNP-1; HP-1; HP1Cleaved into the following 2 chains:HP 1-56Neutrophil defensin 2; Alternative name(s):; HNP-2
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| Protein Family
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Alpha-defensin
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| UniProt Gene Name
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DEFA1
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[Similar Products]
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| UniProt Synonym Gene Names
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DEF1; DEFA2; MRS; HP-1; HP1; HP-2; HP2
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[Similar Products]
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| UniProt Entry Name
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DEF1_HUMAN
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| NCBI Summary for DEFA1
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Defensins are a family of microbicidal and cytotoxic peptides thought to be involved in host defense. They are abundant in the granules of neutrophils and also found in the epithelia of mucosal surfaces such as those of the intestine, respiratory tract, urinary tract, and vagina. Members of the defensin family are highly similar in protein sequence and distinguished by a conserved cysteine motif. The protein encoded by this gene, defensin, alpha 1, is found in the microbicidal granules of neutrophils and likely plays a role in phagocyte-mediated host defense. Several alpha defensin genes are clustered on chromosome 8. This gene differs from defensin, alpha 3 by only one amino acid. This gene and the gene encoding defensin, alpha 3 are both subject to copy number variation. [provided by RefSeq, May 2010]
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| UniProt Comments for DEFA1
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defensin, alpha 1: Defensin 1 and defensin 2 have antibacterial, fungicide and antiviral activities. Has antimicrobial activity against Gram- negative and Gram-positive bacteria. Defensins are thought to kill microbes by permeabilizing their plasma membrane. Belongs to the alpha-defensin family.
Protein type: Secreted; Secreted, signal peptide
Chromosomal Location of Human Ortholog: 8p23.1
Cellular Component: extracellular matrix; extracellular space; Golgi lumen; extracellular region
Biological Process: defense response to Gram-positive bacterium; estrogen receptor signaling pathway; innate immune response in mucosa; killing of cells of another organism; antibacterial humoral response; innate immune response; immune response; chemotaxis; defense response to fungus; defense response to virus
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| Research Articles on DEFA1
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1. Dimerization contributes to some but not all of the activities of HNP1.
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| Precautions
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All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
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| Disclaimer
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While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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| Products associated with DEFA1 elisa kit | Pathways associated with DEFA1 elisa kit |
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| Diseases associated with DEFA1 elisa kit | Organs/Tissues associated with DEFA1 elisa kit |
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ELISA Kit 
