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GATA1 elisa kit :: Chicken Erythroid transcription factor ELISA Kit

Scan QR to view Datasheet Catalog #    MBS283898 GATA1 elisa kit
Unit / Price
48-Strip-Wells  /  $465 +1 FREE 8GB USB
96-Strip-Wells  /  $790 +1 FREE 8GB USB
5x96-Strip-Wells  /  $3,500 +4 FREE 8GB USB
10x96-Strip-Wells  /  $6,655 +8 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Erythroid transcription factor (GATA1), ELISA Kit

★Popular Item★
 Also Known As   

Chicken Erythroid transcription factor (GATA1) ELISA Kit

 Product Synonym Names    ERYF1; GATA-1; GF-1; GF1; NFE1; XLTT; GATA binding protein 1|NF-E1 DNA-binding protein|erythroid transcription factor 1|globin transcription factor 1|transcription factor GATA1
 Product Gene Name   

GATA1 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Request for Current Manual Insert    Request Current Manual
 3D Structure    ModBase 3D Structure for P17678
 Species Reactivity    Chicken
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 Specificity    This assay has high sensitivity and excellent specificity for detection of Chicken GATA1. No significant cross-reactivity or interference between Chicken GATA1 and analogues was observed.
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 Samples    Serum, Plasma, Other biological fluids
 Assay Type    Sandwich
 Sample Volume    1-200 uL
 Precision    Intra-assay Precision (Precision within an assay)
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV
Inter-Assay: CV
 Detection Wavelength    450 nm
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 Preparation and Storage    Store at 2-8 degree C.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of GATA1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for GATA1 purchase    MBS283898 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Erythroid transcription factor (GATA1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing GATA1. The ELISA analytical biochemical technique of the MBS283898 kit is based on GATA1 antibody-GATA1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect GATA1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, GATA1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, Plasma, Other biological fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for GATA1 elisa kit

   Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate GATA1 in samples. An antibody specific for GATA1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyGATA1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for GATA1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of GATA1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
NCBI/Uniprot data below describe general gene information for GATA1. It may not necessarily be applicable to this product.
 NCBI GI #    45382623
 NCBI GeneID    107050548
 NCBI Accession #    NP_990795.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_205464.1 [Other Products]
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 UniProt Primary Accession #    P17678 [Other Products]
 UniProt Related Accession #    P17678 [Other Products]
 Molecular Weight    31,417 Da
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 NCBI Official Full Name    erythroid transcription factor
 NCBI Official Synonym Full Names    GATA binding protein 1 (globin transcription factor 1)
 NCBI Official Symbol    GATA1 [Similar Products]
 NCBI Official Synonym Symbols   
ERYF1; GATA-1; NF-E1a
[Similar Products]
 NCBI Protein Information    erythroid transcription factor; LOW QUALITY PROTEIN: erythroid transcription factor-like
 UniProt Protein Name    Erythroid transcription factor
 UniProt Synonym Protein Names   
Eryf1; GATA-binding factor 1; GATA-1; NF-E1 DNA-binding protein; NF-E1a
 Protein Family    Erythroid transcription factor
 UniProt Gene Name    GATA1 [Similar Products]
 UniProt Synonym Gene Names    ERYF1; GATA-1; NF-E1a [Similar Products]
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 UniProt Comments for GATA1    GATA1: Transcriptional activator which probably serves as a general switch factor for erythroid development. It binds to DNA sites with the consensus sequence [AT]GATA[AG] within regulatory regions of globin genes and of other genes expressed in erythroid cells. May form homodimers or heterodimers with other isoforms. Interacts (via the N-terminal zinc finger) with ZFPM1. Interacts with GFI1B. Interacts with PIAS4; the interaction enhances sumoylation and represses the transactivational activity in a sumoylation-independent manner. Interacts with LMCD1. Interacts with CREBBP; the interaction stimulates acetylation and transcriptional activity in vivo. Interacts with EP300. Erythrocytes. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA-binding; Transcription factor
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 Research Articles on GATA1    1. analysis of the interaction between the transcription factor Gata-1 zinc finger and DNA
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Diseases associated with GATA1 elisa kitOrgans/Tissues associated with GATA1 elisa kit
 Disease Name  Pubmed Publications
 THROMBOCYTOPENIA, X-LINKED, WITH OR WITHOUT DYSERYTHROPOIETIC ANEMIA Antibodies  >676 publications with GATA1 and THROMBOCYTOPENIA, X-LINKED, WITH OR WITHOUT DYSERYTHROPOIETIC ANEMIA
 Leukemia Antibodies  >392 publications with GATA1 and Leukemia
 Leukemia, Myeloid, Acute Antibodies  >242 publications with GATA1 and Leukemia, Myeloid, Acute
 Bone Marrow Diseases Antibodies  >217 publications with GATA1 and Bone Marrow Diseases
 Down Syndrome Antibodies  >174 publications with GATA1 and Down Syndrome
 Nervous System Diseases Antibodies  >148 publications with GATA1 and Nervous System Diseases
 Anemia Antibodies  >145 publications with GATA1 and Anemia
 Thrombocytopenia Antibodies  >63 publications with GATA1 and Thrombocytopenia
 Chromosome Aberrations Antibodies  >46 publications with GATA1 and Chromosome Aberrations
 Cell Transformation, Neoplastic Antibodies  >30 publications with GATA1 and Cell Transformation, Neoplastic
 Organ/Tissue Name  Pubmed Publications
 Mixed Antibodies  >3 publications with GATA1 and Mixed
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