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GPX1 elisa kit :: Chicken GPX1 ELISA Kit

Scan QR to view Datasheet Catalog #    MBS2502532
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $450 +1 FREE 8GB USB
96-Strip-Wells  /  $535 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,255 +2 FREE 8GB USB
10x96-Strip-Wells  /  $3,975 +4 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

GPX1, ELISA Kit

★Popular Item★
 Also Known As   

Chicken GPX1 (Glutathione Peroxidase 1) ELISA Kit

 Product Gene Name   

GPX1 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS2502532 COA    COA PDF
 OMIM    138320
 3D Structure    ModBase 3D Structure for P07203
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 Species Reactivity    Chicken
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 Specificity    This kit recognizes natural and recombinantChickenGPX1. No significant cross-reactivity or interference between ChickenGPX1 and analogues was observed.
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 Samples    Serum, Plasma, Biological Fluids
 Assay Type    Sandwich
 Detection Range    62.5-4000pg/mL
 Sensitivity    Min: 37.5pg/mL; Max: 4000pg/mL
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 Preparation and Storage    Store at 4 degree C.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of GPX1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for GPX1 purchase    MBS2502532 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the GPX1, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 62.5-4000pg/mL in biological research samples containing GPX1, with an estimated sensitivity of Min: 37.5pg/mL; Max: 4000pg/mL. The ELISA analytical biochemical technique of the MBS2502532 kit is based on GPX1 antibody-GPX1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect GPX1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, GPX1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, Plasma, Biological Fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for GPX1 elisa kit

   Intended Uses: This ELISA kit applies to the invitro quantitative determination of ChickenGPX1concentrations in serum, plasma and other biological fluids.

Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to GPX1. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for GPX1and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain GPX1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of GPX1.You can calculate the concentration of GPX1in the samples by comparing the OD of the samples to the standard curve.
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 Typical Testing Data/Standard Curve (for reference only) of GPX1 elisa kit    GPX1 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS2502532. Click to request current manual
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NCBI/Uniprot data below describe general gene information for GPX1. It may not necessarily be applicable to this product.
 NCBI GI #    41406084
 NCBI GeneID    2876
 NCBI Accession #    NP_000572.2 [Other Products]
 NCBI GenBank Nucleotide #    NM_000581.2 [Other Products]
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 UniProt Primary Accession #    P07203 [Other Products]
 UniProt Secondary Accession #    Q7Z5H1; Q9BW12; E9PAS1 [Other Products]
 UniProt Related Accession #    P07203 [Other Products]
 Molecular Weight    10,289 Da
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 NCBI Official Full Name    glutathione peroxidase 1 isoform 1
 NCBI Official Synonym Full Names    glutathione peroxidase 1
 NCBI Official Symbol    GPX1 [Similar Products]
 NCBI Official Synonym Symbols   
GPXD; GSHPX1
[Similar Products]
 NCBI Protein Information    glutathione peroxidase 1; GPx-1; GSHPx-1; cellular glutathione peroxidase
 UniProt Protein Name    Glutathione peroxidase 1
 UniProt Synonym Protein Names   
Cellular glutathione peroxidase
 Protein Family    Hydroperoxy fatty acid reductase
 UniProt Gene Name    GPX1 [Similar Products]
 UniProt Synonym Gene Names    GPx-1; GSHPx-1 [Similar Products]
 UniProt Entry Name    GPX1_HUMAN
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 NCBI Summary for GPX1    This gene encodes a member of the glutathione peroxidase family. Glutathione peroxidase functions in the detoxification of hydrogen peroxide, and is one of the most important antioxidant enzymes in humans. This protein is one of only a few proteins known in higher vertebrates to contain selenocysteine, which occurs at the active site of glutathione peroxidase and is coded by UGA, that normally functions as a translation termination codon. In addition, this protein is characterized in a polyalanine sequence polymorphism in the N-terminal region, which includes three alleles with five, six or seven alanine (ALA) repeats in this sequence. The allele with five ALA repeats is significantly associated with breast cancer risk. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
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 UniProt Comments for GPX1    GPX1: Protects the hemoglobin in erythrocytes from oxidative breakdown. Homotetramer. Interacts with MIEN1. Belongs to the glutathione peroxidase family.

Protein type: EC 1.11.1.9; Lipid Metabolism - arachidonic acid; Oxidoreductase; Other Amino Acids Metabolism - glutathione

Chromosomal Location of Human Ortholog: 3p21.3

Cellular Component: mitochondrion; mitochondrial matrix; cytoplasm; cytosol; nucleus

Molecular Function: selenium binding; glutathione binding; phospholipid-hydroperoxide glutathione peroxidase activity; glutathione peroxidase activity; SH3 domain binding

Biological Process: response to nicotine; fat cell differentiation; blood vessel endothelial cell migration; myoblast proliferation; response to toxin; skeletal muscle fiber development; negative regulation of caspase activity; thermoregulation; regulation of gene expression, epigenetic; vasodilation; response to estradiol stimulus; endothelial cell development; regulation of mammary gland epithelial cell proliferation; response to selenium ion; glutathione metabolic process; sensory perception of sound; UV protection; response to symbiotic bacterium; lipoxygenase pathway; hydrogen peroxide catabolic process; response to folic acid; regulation of neuron apoptosis; response to glucose stimulus; arachidonic acid metabolic process; response to gamma radiation; negative regulation of inflammatory response to antigenic stimulus; aging; protein amino acid oxidation; nucleobase, nucleoside and nucleotide metabolic process; skeletal muscle regeneration; angiogenesis involved in wound healing; purine base metabolic process; response to xenobiotic stimulus; positive regulation of protein kinase B signaling cascade; response to reactive oxygen species; triacylglycerol metabolic process; response to hydrogen peroxide; cell redox homeostasis; heart contraction; response to lipid hydroperoxide; purine nucleotide catabolic process; interaction with symbiont; induction of apoptosis by oxidative stress

Disease: Glutathione Peroxidase Deficiency
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 Research Articles on GPX1    1. Results obtained indicated that both primary GPx-1 sequence and cellular location have a profound impact on cellular biology and offer feasible hypotheses about how expression of distinct GPx-1 alleles can affect cancer risk.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with GPX1 elisa kitPathways associated with GPX1 elisa kit
 Reference Product  PubMed Publications
 GSTP1 elisa kit  >26 publications with GPX1 and GSTP1
 GSR elisa kit  >23 publications with GPX1 and GSR
 GSS elisa kit  >8 publications with GPX1 and GSS
 GSTA1 elisa kit  >5 publications with GPX1 and GSTA1
 GSTM2 elisa kit  >4 publications with GPX1 and GSTM2
 GSTA4 elisa kit  >4 publications with GPX1 and GSTA4
 GSTA2 elisa kit  >1 publications with GPX1 and GSTA2
 Products by Pathway  Pathway Diagram
 Amyotrophic Lateral Sclerosis (ALS) Pathway antibodies  Amyotrophic Lateral Sclerosis (ALS) Pathway Diagram
 Amyotrophic Lateral Sclerosis (ALS) Pathway antibodies  Amyotrophic Lateral Sclerosis (ALS) Pathway Diagram
 Amyotrophic Lateral Sclerosis (ALS) Pathway antibodies  Amyotrophic Lateral Sclerosis (ALS) Pathway Diagram
 Arachidonic Acid Metabolism Pathway antibodies  Arachidonic Acid Metabolism Pathway Diagram
 Arachidonic Acid Metabolism Pathway antibodies  Arachidonic Acid Metabolism Pathway Diagram
 Arachidonic Acid Metabolism Pathway antibodies  Arachidonic Acid Metabolism Pathway Diagram
 Cellular Responses To Stress Pathway antibodies  Cellular Responses To Stress Pathway Diagram
 Detoxification Of Reactive Oxygen Species Pathway antibodies  Detoxification Of Reactive Oxygen Species Pathway Diagram
 Direct P53 Effectors Pathway antibodies  Direct P53 Effectors Pathway Diagram
 Folate Metabolism Pathway antibodies  Folate Metabolism Pathway Diagram
Diseases associated with GPX1 elisa kitOrgans/Tissues associated with GPX1 elisa kit
 Disease Name  Pubmed Publications
 Cardiovascular Diseases Antibodies  >104 publications with GPX1 and Cardiovascular Diseases
 Inflammation Antibodies  >84 publications with GPX1 and Inflammation
 Liver Diseases Antibodies  >55 publications with GPX1 and Liver Diseases
 Heart Diseases Antibodies  >47 publications with GPX1 and Heart Diseases
 Necrosis Antibodies  >45 publications with GPX1 and Necrosis
 Breast Neoplasms Antibodies  >30 publications with GPX1 and Breast Neoplasms
 Lung Neoplasms Antibodies  >28 publications with GPX1 and Lung Neoplasms
 Atherosclerosis Antibodies  >27 publications with GPX1 and Atherosclerosis
 Liver Neoplasms Antibodies  >23 publications with GPX1 and Liver Neoplasms
 Hyperglycemia Antibodies  >22 publications with GPX1 and Hyperglycemia
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >276 publications with GPX1 and Blood
 Liver Antibodies  >213 publications with GPX1 and Liver
 Muscle Antibodies  >106 publications with GPX1 and Muscle
 Brain Antibodies  >93 publications with GPX1 and Brain
 Heart Antibodies  >83 publications with GPX1 and Heart
 Lung Antibodies  >79 publications with GPX1 and Lung
 Vascular Antibodies  >73 publications with GPX1 and Vascular
 Kidney Antibodies  >73 publications with GPX1 and Kidney
 Intestine Antibodies  >44 publications with GPX1 and Intestine
 Prostate Antibodies  >35 publications with GPX1 and Prostate
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